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Enhancing carob flour (Ceratonia siliqua L.) for by-product utilization in food...

Vilas-Boas, Ana Martins; Brassesco, María Emilia; Quintino, Andreia; Medronho, Bruno; Vieira, Margarida; Brandão, Teresa R. S.; Silva, Cristina L. M.

The focus on by-product valorization in the food industry, particularly from the carob pod, underscores a commitment to sustainability and resource efficiency. This fruit, sourced from the leguminous evergreen carob tree (Ceratonia siliqua L.), is renowned for its adaptable flavour and nutritional value, in Mediterranean regions such as Portugal. Its production yields significant by-products, presenting environ...


Bioprospecting bioactive peptides in Halobatrachus didactylus Body Mucus: From ...

Cunha, Marta Fernandez; Coscueta, Ezequiel R.; Brassesco, María Emilia; Almada, Frederico; Gonçalves, David; Pintado, Maria Manuela

Fish body mucus plays a protective role, especially in Halobatrachus didactylus, which inhabits intertidal zones vulnerable to anthropogenic contaminants. In silico predicted bioactive peptides were identified in its body mucus, namely, EDNSELGQETPTLR (HdKTLR), DPPNPKNL (HdKNL), PAPPPPPP (HdPPP), VYPFPGPLPN (HdVLPN), and PFPGPLPN (HdLPN). These peptides were studied in vitro for bioactivities and aggregation be...


Peptide-rich yeast fractions from brewer’s spent yeast: a scalable fractionatio...

Brassesco, María Emilia; Paupério, Ana; Pereira, Carlos D.; Ferreira, João Paulo; Pintado, Manuela

Brewer’s spent yeast (BSY), a significant brewing by-product, poses environmental challenges and opportunities for valorization as a sustainable protein source. This study focuses on transforming BSY into high-value functional ingredients for food applications. A green, sustainable, and scalable process was developed to extract bioactive compounds from BSY at both laboratory and pilot scales, yielding peptide-r...


Developing a high-protein fruit puree using co-products from agro-food processing

Brassesco, María Emilia; Cassoni, Ana C.; Paupério, Ana Isabel; Alegria, Maria João; Correia, Daniela; Figueiredo, Bruna; Pintado, Manuela


Bioprospecting bioactive peptides in halobatrachus didactylus body mucus: from ...

Cunha, Marta Fernandez; Coscueta, Ezequiel R.; Brassesco, María Emilia; Almada, Frederico; Gonçalves, David; Pintado, Maria Manuela

Fish body mucus plays a protective role, especially in Halobatrachus didactylus, which inhabits intertidal zones vulnerable to anthropogenic contaminants. In silico predicted bioactive peptides were identified in its body mucus, namely, EDNSELGQETPTLR (HdKTLR), DPPNPKNL (HdKNL), PAPPPPPP (HdPPP), VYPFPGPLPN (HdVLPN), and PFPGPLPN (HdLPN). These peptides were studied in vitro for bioactivities and aggregation be...


Turning agri-food by-products into functional ingredients: sustainable innovati...

Paupério, Ana; Brassesco, María Emilia; Pereira, Carlos; Ferreira, João Paulo; Pintado, Manuela

Introduction: Environmental Impact: The beverage industry generates large amounts of food by- products, contributing to waste and sustainability challenges. Nutritional & Economic Value: Brewer’s spent yeast (BSY) and rice Okara (OA) are by- products rich in proteins, fibers and bioactive compounds, offering both health benefits and cost-effective opportunities. Contribution to a Sustainable Food System: The ut...


Revealing the potential brewer’s spent yeast: sustainable innovations for alter...

Brassesco, María Emilia; Paupério, Ana Isabel; Coscueta, Ezequiel; Pereira, Carlos D.; Ferreira, João Paulo; Pintado, Manuela

Background: Brewer’s spent yeast (BSY), the second most significant by-product of the brewing industry, represents an abundant biomass resource with considerable environmental implications due to its annual accumulation. This study aims to valorize BSY by-products producing differentiated functional extracts that can be used to formulate protein-rich snacks. By enhancing the value of this by-product, the resear...


Bioaccessibility of novel bioactive peptides from the body mucus of the Lusitan...

Fernandez Cunha, Marta; Coscueta, Ezequiel R.; Brassesco, María Emilia; Almada, Frederico; Gonçalves, David; Pintado, Manuela

The bioprospection of marine resources for drug discovery is receiving increasing attention (1). Adverse marine environmental conditions lead organisms to develop a collection of bioactive molecules for survival (1). Mucus, acting as a first line of defense against pathogens (2), is known to protect fish from the surrounding environment. Our previous studies have already demonstrated the bioactive potential of ...


NanoLC-MS/MS sequencing and in silico analysis of bioactive peptides from Lusit...

Cunha, Marta Fernandez; Coscueta, Ezequiel R.; Brassesco, María Emilia; Almada, Frederico; Gonçalves, David; Pintado, Manuela

The mucus covering a fish's body works as a protective barrier and contains molecules, such as antimicrobial peptides, that defend against pathogens. In this study, two adult Halobatrachus didactylus were captured from the wild in Sesimbra, and mucus was collected by scraping the dorsal-lateral body with a sponge. Our objective was to identify new bioactive peptides in mucus samples via chromatography. SE-HPLC ...


Sustainable bioprospecting and circular economy approach: fractionation and ide...

Cunha, Marta Fernandez; Coscueta, Ezequiel R.; Brassesco, María Emilia; Almada, Frederico; Gonçalves, David; Pintado, Manuela

Bioactive molecules from marine organisms, especially the Halobatrachus didactylus, also known as the Lusitanian toadfish, native to the Portuguese coast, remains largely unexplored. It is important to use sustainable bioprospecting practices to protect both the species and its ecosystem (1). Therefore, by adopting a circular economy approach, we can maximize the sustainable use of marine resources by sampling ...


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