12 documents found, page 1 of 2
Immobilized enzyme cascade for the synthesis of polyaniline
Dencheva, Nadya Vasileva; Guimarães, Sofia Alexandrina da Silva; Braz, Joana Filipa Barros; Denchev, Z.Polyaniline (PANI) is an intrinsically conductive polymer with significant applications, such as in drug delivery, microelectronics, photovoltaic cells, plastic batteries, display devices, and chemical electrodes. PANI is typically synthesized through chemical or electrochemical oxidation polymerization of the ANI monomer. However, these methods have notable drawbacks, including harsh synthesis conditions such ...
Polyamide-based supports for multi-enzyme bio-catalysis
Braz, Joana Filipa Barros; Dencheva, Nadya Vasileva; Denchev, Z.Polyamide-Based Supports for Multi-Enzyme Biocatalysis Joana Barros Braz*1, Nadya Vasileva Dencheva1 and Zlatan Zlatev Denchev1 1: IPC – Institute for Polymers and Composites, University of Minho, Guimarães, Portugal *Presenting author: joanabraz@dep.uminho.pt Abstract text: 250 words max. Enzymatic catalytic processes are known to be much more effective and environmentally friendly as compared to most conventi...
Porous Polyamide microparticles as effective carriers for cascade bio-catalysis
Braz, Joana Filipa Barros; Dencheva, Nadya Vasileva; Denchev, Z.In the last decades, cascade biocatalysis has attracted significant interest from both academia and industry as a promising technology for green and sustainable chemical production. Unlike the stepby-step synthesis, cascade biocatalysts accomplish two or more consecutive reactions in one-pot, thus avoiding isolation and purification of intermediates. Nowadays, numerous drugs and fine chemicals, as well as some ...
Polyamide microparticles as promising enzyme carriers and their application in ...
Braz, Joana Filipa Barros; Dencheva, Nadya Vasileva; Oliveira, Sandra Cristina Gomes; Araújo, Samuel; Denchev, Z.The widespread use of industrial-scale processes employing enzymatic catalysts is still hindered by the inactivation of free enzymes due to various denaturant agents and the difficulty, or even impossibility, of their recovery from the reaction medium after use. To address these limitations, a new strategy has been proposed, centered on polymer-assisted biocatalysts created through the non-covalent immobilizati...
Fast, multiple-use optical biosensor for point-of-care glucose detection with m...
Braz, Joana Filipa Barros; Dencheva, Nadya Vasileva; Tohidi, Shafagh Dinparast; Denchev, Z.Non-invasive glucose determination provides major advantages in health monitoring and protection. It enables widespread point-of-care testing, which is affordable, sensitive, specific, rapid and equipment-free. This work reports on the development and analytical performance of a colorimetric biosensor in detecting glucose in human urine. Highly porous polyamide microparticles were synthesized as the support for...
Smart-phone read optical biosensor for glucose determination using polymer-assi...
Braz, Joana Filipa Barros; Dencheva, Nadya Vasileva; Tohidi, Shafagh Dinparast; Denchev, Z.Diabetes mellitus, commonly known as diabetes, is an urgent global health crisis. These motivated the widespread development of non-invasive point-of-care (POCT) technologies for continuous, real-time glucose monitoring in biofluids such as urine. The sensitive colorimetric method is the easiest and fastest method that produces a distinctive color change to the target analyte and is observed by the naked eye, e...
Novel polymer-assisted organic-inorganic hybrid nanoflowers for cascade biocata...
Braz, Joana Filipa Barros; Dencheva, Nadya Vasileva; Dencjhev, Zlatan Z.ABSTRACT (250-300 words) Background: There is increasing interest towards multienzyme complexes, in which several enzymes are co-immobilized on the same support used to catalyze cascade reactions. Major advantage of these complexes is that the enzymes´ active sites are in close proximity, minimizing the diffusion of the intermediates thereby enhancing the overall reaction efficiency. The compartmentalization of...
Synthesis and properties of neat, hybrid, and copolymeric polyamide 12 micropar...
Dencheva, Nadya Vasileva; Braz, Joana Filipa Barros; Denchev, Z.Neat and hybrid polyamide 12 (PA12) based powders are important raw materials for additive manufacturing. This study describes a facile method for the synthesis of pure, hybrid and copolymeric microparticulate materials based on one-pot activated anionic ring-opening polymerization (AAROP) of laurolactam in solution. The results reveal the possibility to obtain in good yields, for reaction times of 2 h and temp...
Spatial colocalization of multienzymes by polymer assisted organic-inorganic na...
Braz, Joana Filipa Barros; Dencheva, Nadya Vasileva; Denchev, Z.Multienzyme complexes in which several enzymes are co-immobilized on the same support used to catalyze multistep or cascade reactions have recently received continuous interest. The major advantage of the multienzyme co-immobilization is that the enzymes´ active sites are in close proximity minimizing the diffusion of the intermediates, thereby enhancing the overall reaction efficiency. The spatial organization...
Magnetically responsive PA6 microparticles with immobilized laccase show high c...
Dencheva, Nadya Vasileva; Oliveira, Sandra Cristina Gomes; Braz, Joana Filipa Barros; Getya, Dariya; Malfois, Marc; Denchev, Z.; Gitsov, IvanHerewith we report the first attempt towards non-covalent immobilization of <i>Trametes versicolor</i> laccase on neat and magnetically responsive highly porous polyamide 6 (PA6) microparticles and their application for catechol oxidation. Four polyamide supports, namely neat PA6 and such carrying Fe, phosphate-coated Fe and Fe<sub>3</sub>O<sub>4</sub> cores were synthesized in suspension by activated anionic r...