5 documents found, page 1 of 1
CkP1 bacteriophage, a S16-like myovirus that recognizes Citrobacter koseri lipo...
Oliveira, Hugo Alexandre Mendes; Santos, Sílvio Roberto Branco; Pires, Diana Priscila Penso; Boeckaerts, Dimitri; Pinto, Graça; Domingues, RitaCitrobacter koseri is an emerging Gram-negative bacterial pathogen, which causes urinary tract infections. We isolated and characterized a novel S16-like myovirus CKP1 (vB\_CkoM\\_CkP1), infecting C. koseri. CkP1 has a host range covering the whole C. koseri species, i.e., all strains that were tested, but does not infect other species. Its linear 168,463-bp genome contains 291 coding sequences, sharing sequenc...
CkP1 is a novel S16-like broad-host-range myovirus that recognizes Citrobacter ...
Oliveira, Hugo Alexandre Mendes; Santos, Sílvio Roberto Branco; Pires, Diana P.; Pinto, Graça; Domingues, Rita; Briers, Yves; Schmelcher, MathiasCitrobacter spp. is an emerging Gram-negative bacterial pathogen. The genus is currently divided in several species, being Citrobacter koseri (formerly named Citrobacter diversus) one of the most common isolated species in human clinical specimens. C. koseri can cause localized (urinary track, wounds infection, pneumonia, meningitis) and systemic life-threatening diseases (bacteremia, septicemia), being neonate...
Characterization and genome sequencing of a Citrobacter freundii phage CfP1 har...
Oliveira, Hugo; Pinto, Graça; Oliveira, A.; Oliveira, Carla; Faustino, Maria Alberta; Briers, Yves; Domingues, Lucília; Azeredo, JoanaCitrobacter spp., although frequently ignored, is emerging as an important nosocomial bacterium able to cause various superficial and systemic life-threatening infections. Considered to be hard-to-treat bacterium due to its pattern of high antibiotic resistance, it is important to develop effective measures for early and efficient therapy. In this study, the first myovirus (vB_CfrM_CfP1) lytic for Citrobacter f...
Engineered endolysin-based "artilysins" to combat multidrug-resistant gram-nega...
Briers, Yves; Walmagh, Maarten; Van Puyenbroeck, Victor; Cornelissen, Anneleen; Cenens, William; Aertsen, Abram; Oliveira, Hugo; Azeredo, JoanaThe global threat to public health posed by emerging multidrug-resistant bacteria in the past few years necessitates the development of novel approaches to combat bacterial infections. Endolysins encoded by bacterial viruses (or phages) represent one promising avenue of investigation. These enzyme-based antibacterials efficiently kill Gram-positive bacteria upon contact by specific cell wall hydrolysis. However...
Characterization of modular bacteriophage endolysins from Myoviridae phages OBP...
Walmagh, Maarten; Briers, Yves; Santos, Sílvio Roberto Branco; Azeredo, Joana; Lavigne, RobPeptidoglycan lytic enzymes (endolysins) induce bacterial host cell lysis in the late phase of the lytic bacteriophage replication cycle. Endolysins OBPgp279 (from Pseudomonas fluorescens phage OBP), PVP-SE1gp146 (Salmonella enterica serovar Enteritidis phage PVP-SE1) and 201φ2-1gp229 (Pseudomonas chlororaphis phage 201φ2-1) all possess a modular structure with an N-terminal cell wall binding domain and a C-ter...