5 documents found, page 1 of 1
Rationally guided improvement of NOV1 Dioxygenase for the conversion of lignin-...
De Simone, Mario; Alvigini, Laura; Alonso-Cotchico, Lur; Brissos, Vânia; Caroli, Jonatan; Lucas, Maria Fátima; Monza, Emanuele; Melo, EduardoBiocatalysis is a key tool in both green chemistry and biorefinery fields. NOV1 is a dioxygenase that catalyzes the one-step, coenzyme-free oxidation of isoeugenol into vanillin and holds enormous biotechnological potential for the complete valorization of lignin as a sustainable starting material for biobased chemicals, polymers, and materials. This study integrates computational, kinetic, structural, and biop...
Unveiling molecular details behind improved activity at neutral to alkaline pH ...
Borges, Patrícia T.; Silva, Diogo; Silva, Tomás F.D.; Brissos, Vânia; Cañellas, Marina; Lucas, Maria Fátima; Masgrau, Laura; Melo, EduardoDyP-type peroxidases (DyPs) are microbial enzymes that catalyze the oxidation of a wide range of substrates, including synthetic dyes, lignin-derived compounds, and metals, such as Mn2+ and Fe2+, and have enormous biotechnological potential in biorefineries. However, many questions on the molecular basis of enzyme function and stability remain unanswered. In this work, high-resolution structures of PpDyP wild-t...
The VASelfCare T2D project plan : fostering innovation through the StartUp Rese...
Brissos, Vânia; Santos, Carolina; Santos, Jorge Miguel; Guerreiro, Mara PereiraDiabetes represents a tremendous burden at a global scale. Changing and sustaining desirable health behaviours, such as engaging in a healthy diet, physical activity and taking prescribed medication, is key to achieve better health outcomes in people with type 2 diabetes (T2D). This paper describes the first stage of a plan to translate an innovative a mobile application to facilitate self-care of older adults ...
Engineering a bacterial DyP-Type peroxidase for enhanced oxidation of lignin-re...
Brissos, Vânia; Tavares, Diogo; Sousa, Ana Catarina; Robalo, Maria Paula; Martins, Lígia O.Dye-decolorizing peroxidases (DyPs) are a family of microbial heme-containing peroxidases that show important properties for lignocellulose biorefineries due to their ability to oxidize lignin-related compounds. Directed evolution was used to improve the efficiency of the bacterial PpDyP from Pseudomonas putida MET94 for phenolic compounds. Three rounds of random mutagenesis by error prone PCR of the ppDyP gene...
Improving kinetic or thermodynamic stability of an azoreductase by directed evo...
Brissos, Vânia; Gonçalves, Nádia; Melo, Eduardo; Martins, Lígia O.Protein stability arises from a combination of factors which are often difficult to rationalise. Therefore its improvement is better addressed through directed evolution than by rational design approaches. In this study, five rounds of mutagenesis/recombination followed by high-throughput screening (approximate to 10,000 clones) yielded the hit 1B6 showing a 300-fold higher half life at 50 degrees C than that e...