43 documents found, page 1 of 5
Unravelling the triad of neuroinvasion, neurodissemination, and neuroinflammati...
Calado, Marta; Ferreira, Rita; Pires, David; Santos-Costa, Quirina; Anes, Elsa; Brites, Dora; Azevedo-Pereira, José MiguelSince the identification of human immunodeficiency virus type 1 (HIV-1) in 1983, many improvements have been made to control viral replication in the peripheral blood and to treat opportunistic infections. This has increased life expectancy but also the incidence of age-related central nervous system (CNS) disorders and HIV-associated neurodegeneration/neurocognitive impairment and depression collectively refer...
Spatiotemporal dysregulation of neuron-glia related genes and pro-/anti-inflamm...
Ianni, Marta; Corraliza-Gomez, Miriam; Costa-Coelho, Tiago; Ferreira-Manso, Mafalda; Inteiro-Oliveira, Sara; Alemãn-Serrano, Nuno; Sebastião, Ana MAlzheimer's disease (AD), the leading cause of dementia, is a multifactorial disease influenced by aging, genetics, and environmental factors. miRNAs are crucial regulators of gene expression and play significant roles in AD onset and progression. This exploratory study analyzed the expression levels of 28 genes and 5 miRNAs (miR-124-3p, miR-125b-5p, miR-21-5p, miR-146a-5p, and miR-155-5p) related to AD patholo...
Differences in Immune-Related Genes Underlie Temporal and Regional Pathological...
Fernandes, Adelaide; Caldeira, Cláudia; Cunha, Carolina; Ferreiro, Elisabete; Vaz, Ana Rita; Brites, DoraThe prevalence of Alzheimer's disease (AD), the most common cause of age-associated dementia, is estimated to increase over the next decades. Evidence suggests neuro-immune signaling deregulation and risk genes beyond the amyloid-β (Aβ) deposition in AD pathology. We examined the temporal profile of inflammatory mediators and microglia deactivation/activation in the brain cortex and hippocampus of 3xTg-AD mice ...
Intrathecal injection of the secretome from ALS motor neurons regulated for miR...
Barbosa, Marta; Santos, Marta; de Sousa, Nídia; Silva, Sara Carina Duarte; Vaz, Ana Rita; Salgado, A. J.; Brites, DoraAmyotrophic lateral sclerosis (ALS) is a neurodegenerative disease with short life expectancy and no effective therapy. We previously identified upregulated miR-124 in NSC-34-motor neurons (MNs) expressing human SOD1-G93A (mSOD1) and established its implication in mSOD1 MN degeneration and glial cell activation. When anti-miR-124-treated mSOD1 MN (preconditioned) secretome was incubated in spinal cord organotyp...
S100B inhibition protects from chronic experimental autoimmune encephalomyelitis
Barros, Catarina; Barateiro, Andreia; Neto, Alexandre; Soromenho, Beatriz; Basto, Afonso; Mateus, Joana; Xapelli, Sara; Sebastião, Ana M; Brites, DoraStudies have correlated excessive S100B, a small inflammatory molecule, with demyelination and associated inflammatory processes occurring in multiple sclerosis. The relevance of S100B in multiple sclerosis pathology brought an emerging curiosity highlighting its use as a potential therapeutic target to reduce damage during the multiple sclerosis course, namely during inflammatory relapses. We examined the rele...
Sedentary life and reduced mastication impair spatial learning and memory and d...
Mendes, Fab?ola de Carvalho Chaves de Siqueira; Paix?o, Luisa Taynah Vasconcelos Barbosa; Diniz, Daniel Guerreiro; Anthony, Daniel Clive; Brites, DoraBrazilian Research Council CNPq (grant number 475677/2008-0) Funda??o Amaz?nia Paraense de Amparo ? Pesquisa (FAPESPA, grant number 136/08). Funda??o de Amparo e Desenvolvimento da Pesquisa (FADESP) and the Pr?-Reitoria de Pesquisa e P?s-Gradua??o (PROPESP/UFPA) Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior (CAPES). Programa Nacional de Coopera??o Acad?mica da Amaz?nia ? DRI (Processo CAPES No. 88...
Microglial morphology across distantly related species: phylogenetic, environme...
Carvalho-Paulo, Dario; Torres Neto, Jo?o Bento; Santos Filho, Carlos; Oliveira, Thais Cristina Galdino de; Sousa, Aline AmaralFundac??o de Amparo ? Pesquisa do Para? ? FADESP/Pro?-Reitoria de Pesquisa e Po?s-Graduac??o da Universidade Federal do Para? ? PROPESP Edital 2020-PIAPA; Coordenac??o de Aperfeic?oamento de Pessoal de N?vel Superior ? ? CAPES ? Pro?-Amazo?nia, Grant No. 3311/2013; Programa Ciencias do Mar II; The Canadian Bureau for ? International Education (CBIE); Brazilian Research Council ? CNPq Grant No. 307749/2004-5 and...
Neuronal dynamics and miRNA signaling differ between SH-SY5Y APPSwe and PSEN1 M...
Garcia, Gonçalo; Pinto, Sara; Cunha, Mar; Fernandes, Adelaide; Koistinaho, Jari; Brites, DoraNeuronal miRNA dysregulation may have a role in the pathophysiology of Alzheimer’s disease (AD). miRNA(miR)-124 is largely abundant and a critical player in many neuronal functions. However, the lack of models reliably recapitulating AD pathophysiology hampers our understanding of miR-124’s role in the disease. Using the classical human SH-SY5Y-APP695 Swedish neuroblastoma cells (SH-SWE) and the PSEN1 mutant iP...
Recovery of depleted miR-146a in ALS cortical astrocytes reverts cell aberranci...
Barbosa, Marta; Gomes, Cátia; Sequeira, Catarina; Gonçalves-Ribeiro, Joana; Pina, Carolina Campos; Carvalho, Luís A.; Moreira, Rui; Vaz, Sandra H.Reactive astrocytes in Amyotrophic Lateral Sclerosis (ALS) change their molecular expression pattern and release toxic factors that contribute to neurodegeneration and microglial activation. We and others identified a dysregulated inflammatory miRNA profile in ALS patients and in mice models suggesting that they represent potential targets for therapeutic intervention. Such cellular miRNAs are known to be relea...
Downregulated glia interplay and increased miRNA-155 as promising markers to tr...
Cunha, Carolina; Santos, Catarina; Gomes, Cátia; Fernandes, Adelaide; Marcal Correia, Alexandra; Sebastião, Ana M; Vaz, Ana R; Brites, DoraAmyotrophic lateral sclerosis (ALS) is a fatal neurodegenerative disease of unknown cause. Absence of specific targets and biomarkers compromise the development of new therapeutic strategies and of innovative tools to stratify patients and assess their responses to treatment. Here, we investigate changes in neuroprotective-neuroinflammatory actions in the spinal cord of SOD1 G93A mice, at presymptomatic and sym...