Numerical zymotaxonomy and variability of the internal transcribed spacers (ITS) between the small and large subunits of the rRNA genes were used to examine strain variation and relationships in natural populations of Leishmania (Viannia) braziliensis. A total of 101 strains from distinct hosts and Brazilian geographic regions were assigned to 15 zymodemes clustered in two major genetic groups. The great number...
This work was supported by Funda??o de Amparo ? Pesquisa do Estado de S?o Paulo (grant 2015/22075-8 and fellowship 2016/02622-7 to C.M.S.), by CAPES (fellowship to C.M.S.), by Conselho Nacional de Pesquisa (research fellowship to H.G.), and by Laborat?rios de Investiga??o M?dica (LIM-38-HC, FMUSP). This research was also supported by funding from the Bill and Melinda Gates Foundation under grants OPP49932 and O...
Visceral leishmaniasis, associated with HIV/AIDS coinfection, is becoming a more aggressive disease, complicating an accurate prognosis. A 21-year-old HIV-positive female presenting with clinical features of visceral leishmaniasis was enrolled in this study. Bone marrow cytology, Novy-MacNeal-Nicolle culture and kDNA PCR of peripheral blood were all positive. Typing methods, multilocus enzyme electrophoresis an...