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Standardization of in vitro digestibility and DIAAS method based on the static ...

Egger, Lotti; Sousa, Raquel; Recio, Isidra; Abbühl, Lychou; Brügger, Cédric; Hernández, Marta Santos; Macierzanka, Adam; Szumala, Patrycja

Background: The FAO recommends the digestible indispensable amino acid score (DIAAS) as the measure for protein quality, for which the true ileal digestibility needs to be assessed in humans or pigs. However, due to high costs and ethical concerns, the FAO strongly encourages as well the development of validated in vitro methods, which complement the in vivo experiments. Method: Recently, an in vitro workflow, ...


INFOGEST static in vitro simulation of gastrointestinal food digestion

Brodkorb, André; Egger, Lotti; Alminger, Marie; Alvito, Paula; Assunção, Ricardo; Ballance, Simon; Bohn, Torsten; Bourlieu-Lacanal, Claire

Developing a mechanistic understanding of the impact of food structure and composition on human health has increasingly involved simulating digestion in the upper gastrointestinal tract. These simulations have used a wide range of different conditions that often have very little physiological relevance, and this impedes the meaningful comparison of results. The standardized protocol presented here is based on a...


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