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Application of BRED technology to construct recombinant D29 reporter phage expr...
Silva, Joas L. da; Piuri, Mariana; Broussard, Gregory; Marinelli, Laura J.; Bastos, Gisele M.; Hirata, Rosario D.C.; Hatfull, Graham F.Bacteriophage Recombineering of Electroporated DNA (BRED) has been described for construction of gene deletion and point mutations in mycobacteriophages. Using BRED, we inserted a Phsp60-egfp cassette (1143 bp) into the mycobacteriophage D29 genome to construct a new reporter phage, which was used for detection of mycobacterial cells. The cassette was successfully inserted and recombinant mycobacteriophage puri...
Date: 2013
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Origin: Oasisbr