14 documents found, page 1 of 2
Stakeholders’ perspectives on hosting large-scale sports events
Dionísio, P.; Brochado, A.; Leal, C.; Bouchet, A.Special events, in general, and sports events, in particular, are a major focus and revenue generator for cities that host these events. They are time-specific, and they can have a variety of short- or long-term, positive or negative impacts, which contribute to both beneficial or harmful outcomes. If prolonged over time, these outcomes have been called “legacies.” This study sought to explore key stakeholders’...
Assessment of ecotoxicological effects of ciprofloxacin in Daphnia magna: life-...
Nunes, B.; Leal, C.; Rodrigues, S.; Antunes, S. C.Antibiotics (e.g. ciprofloxacin) have been detected in surface water and groundwater for several decades. In order to understand the potential impact of the continuous exposure of aquatic organisms to ciprofloxacin, a chronic assay was carried out with Daphnia magna. This approach allowed evaluation of the effects of ciprofloxacin on life-history and sub-individual parameters (antioxidant status and metabolic r...
Quantitative image analysis of polyhydroxyalkanoates inclusions from microbial ...
Amaral, A. Luís; Abreu, H.; Leal, C.; Mesquita, Daniela P.; Castro, L. M.; Ferreira, Eugénio C.Polyhydroxyalkanoates (PHAs) produced from mixed microbial cultures (MMC), regarded as potential substitutes of petrochemical plastics, can be found as intracellular granules in various microorganisms under limited nutrient conditions and excess of carbon source. PHA is traditionally quantified by laborious and time-consuming chromatography analysis, and a simpler and faster method to assess PHA contents from M...
Polyhydroxyalkanoate granules quantification in mixed microbial cultures using ...
Mesquita, D. P.; Amaral, A. L.; Leal, C.; Oehmen, Adrian; Reis, Maria A. M.; Ferreira, Eugénio C.Polyhydroxyalkanoates (PHA) can be produced and intracellularly accumulated as inclusions by mixed microbial cultures (MMC) for bioplastic production and in enhanced biological phosphorus removal (EBPR) systems. Classical methods for PHA quantification use a digestion step prior to chromatography analysis, rendering them labor intensive and time-consuming. The present work investigates the use of two quantitati...
Monitoring intracellular polyphosphate accumulation in enhanced biological phos...
Mesquita, D. P.; Amaral, A. L.; Leal, C.; Carvalheira, Mónica; Cunha, J. R.; Oehmen, Adrian; Reis, Maria A. M.; Ferreira, Eugénio C.A rapid methodology for intracellular storage polyphosphate (poly-P) identification and monitoring in enhanced biological phosphorus removal (EBPR) systems is proposed based on quantitative image analysis (QIA). In EBPR systems, 4′,6-diamidino-2-phenylindole (DAPI) is usually combined with fluorescence in situ hybridization to evaluate the microbial community. The proposed monitoring technique is based on a QIA...
In vitro and in vivo biological behavior of CDHA/OCP/β-TCP scaffold
Minarelli-Gaspar, A. M. [UNESP]; Saska, S. [UNESP]; Jimenez, J.; da Cunha, L. R. [UNESP]; Bolini, Paulo Domingos André [UNESP]; Leal, C.Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-27T11:22:48Z No. of bitstreams: 0; Made available in DSpace on 2014-05-27T11:22:48Z (GMT). No. of bitstreams: 0 Previous issue date: 2008-01-22; Item merged in doublecheck by Felipe Arakaki (fe.arakaki@gmail.com) on 2015-11-11T11:48:55Z Item was identical to item(s): 16236, 69772 at handle(s): http://hdl.handle.net/11449/16598,...
Prediction of intracellular storage polymers using quantitative image analysis ...
Mesquita, D. P.; Leal, C.; Cunha, J. R.; Oehmen, Adrian; Amaral, A. L.; Reis, Maria A. M.; Ferreira, Eugénio C.The present study focuses on predicting the concentration of intracellular storage polymers in enhanced biological phosphorus removal (EBPR) systems. For that purpose, quantitative image analysis techniques were developed for determining the intracellular concentrations of PHA (PHB and PHV) with Nile blue and glycogen with aniline blue staining. Partial least squares (PLS) were used to predict the standard anal...
Intracellular poly-P assessment by DAPI staining and image analysis
Amaral, A. L.; Mesquita, D. P.; Leal, C.; Carvalheira, Mónica; Oehmen, Adrian; Reis, Maria A. M.; Ferreira, Eugénio C.In wastewater treatment, enhanced biological phosphorus removal (EBPR) is considered a well-established process to remove phosphate (P). EBPR is based on the activity of polyphosphate-accumulating organisms (PAOs) able to take up and store large amounts of P as intracellular (poly-P) granules. However, monitoring poly-P in mixed cultures is usually performed by a laborious and time consuming off-line chemical a...
Prediction of intracellular storage polymers using quantitative image analysis ...
Mesquita, D. P.; Leal, C.; Cunha, J. R.; Oehmen, Adrian; Amaral, A. L.; Reis, Maria A. M.; Ferreira, Eugénio C.Intracellular polymers, such as polyhydroxyalkanoates (PHA) synthesized by microorganisms for energy and carbon storage, can be commercially used as biodegradable plastics in a wide range of applications. The possibility of obtaining high PHA contents (where the most common monomers found are poly-hydroxybutyrate (PHB) and poly-hydroxyvalerate (PHV)) from inexpensive inocula and raw materials emerges as a promi...
DNA−Surfactant Complexes at Solid Surfaces
Eskilsson, K.; Leal, C.; Lindman, B.; Miguel, M.; Nylander, T.In this work, we report on the adsorption of complexes between DNA of different molecular weight and a cationic surfactant, cetyltrimethylammonium bromide (CTAB), on hydrophobized and hydrophilic negatively charged silica surfaces as measured by ellipsometry. We will demonstrate how the adsorption is affected by the state of the DNA−surfactant complexes formed in bulk solution. High molecular weight DNA molecul...