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How parasitism genes are regulated: a motif to search for genes regulators in t...

Mendonça, Madalena; Vicente, Cláudia S. L.; Barbosa, Pedro; Velada, Isabel; Vieira, Paulo; Faria, Jorge; Santos, Margarida; Nóbrega, Filomena

Plant-parasitic nematodes threaten global agricultural and forestry systems. The search for new control strategies in line with the EU’s sustainability goals highlight significant knowledge gaps. Like all other plant pathogens, plant-parasitic nematodes deliver several parasitism proteins (effectors) into the host plant to cause disease. NemaWAARS project focuses on mechanism(s) of regulation and gene control e...


From plant survival under severe stress to anti-viral human defense - a perspec...

Arnholdt-Schmitt, Birgit; Mohanapriya, Gunasekaran; Bharadwaj, Revuru; Noceda, Carlos; Macedo, Elisete; Sathishkumar, Ramalingam; Gupta, Kapuganti

Reprogramming of primary virus-infected cells is the critical step that turns viral attacks harmful to humans by initiating super-spreading at cell, organism and population levels. To develop early anti-viral therapies and proactive administration, it is important to understand the very first steps of this process. Plant somatic embryogenesis (SE) is the earliest and most studied model for de novo programming u...


Adaptive Reprogramming During Early Seed Germination Requires Temporarily Enhan...

Bharadwaj, Revuru; Noceda, Carlos; Mohanapriya, Gunasekharan; Kumar, Sarma Rajeev; Thiers, Karine Leitão Lima; Costa, José Hélio; Macedo, Elisete Santos

Plants respond to environmental cues via adaptive cell reprogramming that can affect whole plant and ecosystem functionality. Microbiota constitutes part of the inner and outer environment of the plant. This Umwelt underlies steady dynamics, due to complex local and global biotic and abiotic changes. Hence, adaptive plant holobiont responses are crucial for continuous metabolic adjustment at the systems level. ...


From Plant Survival Under Severe Stress to Anti-Viral Human Defense - A Perspec...

Arnholdt-Schmitt, Birgit; Mohanapriya, Gunasekaran; Bharadwaj, Revuru; Noceda, Carlos; Macedo, Elisete Santos; Sathishkumar, Ramalingam

Reprogramming of primary virus-infected cells is the critical step that turns viral attacks harmful to humans by initiating super-spreading at cell, organism and population levels. To develop early anti-viral therapies and proactive administration, it is important to understand the very first steps of this process. Plant somatic embryogenesis (SE) is the earliest and most studied model for de novo programming u...


Laser Microdissection of Specific Stem-Base Tissue Types from Olive Microcuttin...

Velada, Isabel; Menéndez, Esther; Teixeira, Rita; Cardoso, Hélia; Peixe, Augusto

Higher plants are composed of different tissue and cell types. Distinct cells host different biochemical and physiological processes which is reflected in differences in gene expression profiles, protein and metabolite levels. When omics are to be carried out, the information provided by a specific cell type can be diluted and/or masked when using a mixture of distinct cells. Thus, studies performed at the cell...


Authenticity of Portuguese olive oils assured by the use of molecular markers.

Cardoso, Hélia; Dias, Andreia; Marques, Ana Catarina; Velada, Isabel; Carvalho, Teresa; Nobre, Tânia; Cabrita, Maria João

Olive (Olea europaea L. subsp. europaea var. europaea) is one of the oldest tree crops in the Mediterranean basin which has recently be gaining attention due to the nutritional and health properties of its fruit and derived oil. The demand for high quality olive oils have been directly implicated on the increase of typical marks, awarded to high-quality olive oils produced from local varieties grown in well-defi...


Uso de marcadores moleculares aplicados à rastreabilidade dos Azeites.

Marques, Ana Catarina; Dias, Andreia; Cardoso, Hélia; Velada, Isabel; Carvalho, Teresa; Nobre, Tânia; Cabrita, Maria João

A oliveira (Olea europaea L.) é uma das mais antigas culturas arbóreas da bacia do Mediterrânico (Raieta et al. 2015), com uma importância económica, social e cultural inegável na região. A qualidade e características do azeite depende significativamente da escolha da variedade que lhe dá origem. Algumas cultivares de azeite são reconhecidas como de alta qualidade, e derivam de áreas geográficas bem definidas; ...


Establishment of a Taqman-based approach to monitor Fusarium spp. Airbone spores.

Cardoso, Hélia; Campos, Maria Doroteia; Tomero, Bruno; Felix, Maria Rosário; Velada, Isabel; Peixe, Augusto; Penedos, C; Nunes, C; Morais-Almeida, M

Aerobiological studies provide important information about the biological particles present in the air. Monitoring the presence of airborne fungal spores can help farmers to prevent the onset of fungal diseases that may affect both quantity and quality of crops. The fungi Fusarium spp. are among the most important phytopathogenic fungal communities with high impact at regional level by affecting important cultu...


Genome-wide analysis and expression profile of PIN-formed auxin carrier genes d...

Velada, Isabel; Cardoso, Hélia; Peixe, Augusto

Olive (Olea europaea subsp. europaea var. europaea L.) comprises several cultivars with reduced capacity to be propagated due its recalcitrant behaviour upon adventitious rooting (AR) stimulus. This prevents their propagation and consequently their availability in the nurseries. By overcoming the difficult-to-root behaviour it will be possible to increase the number of cultivars available for new orchards and c...


Establishment of a reliable protocol for gDNA extraction from olive oil.

Dias, Andreia; Marques, Ana Catarina; Velada, Isabel; Carvalho, Teresa; Nobre, Tânia; Cardoso, Hélia; Cabrita, Maria João

With no possibility to reach in quantity the production of countries with large areas of olive orchards, as a small country, Portugal needs to define a strategy for valuing the high quality and specificities of its olive oil. No doubt the focus must be on the valorization of the Portuguese cultivars, the key factor in determining the singularity of the produced olive oils. Fraud detection, as the use of non-Por...


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