11 documents found, page 1 of 2
Understanding lactoferrin multifunctionality by analyzing the full spectrum of ...
Ferreira, Sofia; Guedes, Joana P.; Rocha, Juliana F.; Sousa, Sérgio F.; Rodrigues, L. R.; Côrte-Real, Manuela; Soares, Pedro; Cátia S. PereiraLactoferrin (LF) is a multifunctional protein involved in critical biological processes such as immune response, antimicrobial activity, and iron regulation [1]. Its diverse roles include inhibiting inflammation, infections, and carcinogenesis [2], as well as modulating cellular processes by targeting proton-pumping ATPases such as V-ATPases in cancer cells [3], F- ATPases in bacteria [4], and P-ATPases in yeas...
Current and novel approaches in yeast cell death research
Chaves, Susana Alexandra Rodrigues; Rego, António; Cátia S. Pereira; Sousa, Maria João; Côrte-Real, ManuelaThe study of cell death mechanisms in fungi, particularly yeasts, has gained substantial interest in recent decades driven by the potential for biotechnological advancements and therapeutic interventions. Examples include the development of robust yeast strains for industrial fermentations and high-value compound production, novel food preservation strategies against spoilage yeasts, and the identification of t...
Exosome-mediated MEK1 silencing is a promising approach against triple negative...
Ferreira, Débora; Cátia S. Pereira; Costa, Marta; Afonso, Julieta; Longatto, Adhemar; Baltazar, Fátima; Kalluri, Raghu; Moreira, João N.[Excerpt] Introduction: Breast cancer is a major public health problem worldwide being the most diagnosed cancer in women [1] . Triple negative breast cancer (TNBC) represents 10-20% of all breast cancers and is characterized by the absence of hormone receptors (progesterone and estrogen) and lack of expression of epidermal growth factor receptor 2. Furthermore, it is also described to have poor prognosis due t...
Synthesis and cytotoxicity assessment of citrate-coated calcium and manganese f...
Andrade, Raquel; Ferreira, Débora; Veloso, Sérgio R. S.; Cátia S. Pereira; Castanheira, Elisabete M. S.; Côrte-Real, Manuela; Rodrigues, L. R.Calcium-doped manganese ferrite nanoparticles (NPs) are gaining special interest in the biomedical field due to their lower cytotoxicity compared with other ferrites, and the fact that they have improved magnetic properties. Magnetic hyperthermia (MH) is an alternative cancer treatment, in which magnetic nanoparticles promote local heating that can lead to the apoptosis of cancer cells. In this work, manganese/...
Identification of novel aptamers targeting cathepsin B-overexpressing prostate ...
Pereira, Ana Cláudia Santos; Pina, André F.; Sousa, Diana Andrade; Ferreira, Débora Carina Gonçalves Abreu; Cátia S. PereiraIncreased levels of cathepsin B (CatB), a cysteine protease, have been associated with different types of tumors, including prostate cancer. Hence, the identification of novel targeting ligands homing CatB may be a promising approach for the development of CatB-targeted therapies. In this work, a methodology called systematic evolution of ligands by EXponential enrichment (SELEX) was used to generate and isolat...
Image processing tool for the detection and quantification of xylanase activity...
Sousa, Joana Raquel Fernandes; Cátia S. Pereira; Costa, Ângela Maria Araújo; Guiet, Andréia Oliveira Santos; Gudiña, Eduardo JoséThe vast diversity of unexplored microbial communities inhabiting the planet drives the continuous screening for promising biocatalysts. Until recently, the strategies to find new microorganisms and their enzymes were mainly focused on laboratory studies of pure microbial cultures. However, a great amount of environmental microorganisms cannot be cultivated under laboratorial conditions [1]. Metagenomics has em...
Acetic acid triggers cytochrome c release in yeast heterologously expressing hu...
Guedes, Joana P.; Baptista, Vitória; Cátia S. Pereira; Sousa, Maria João; Manon, Stéphen; Chaves, Susana R.; Côrte-Real, ManuelaProteins of the Bcl-2 protein family, including pro-apoptotic Bax and anti-apoptotic Bcl-xL, are critical for mitochondrial-mediated apoptosis regulation. Since yeast lacks obvious orthologs of Bcl-2 family members, heterologous expression of these proteins has been used to investigate their molecular and functional aspects. Active Bax is involved in the formation of mitochondrial outer membrane pores, through ...
Emerging insights on the role of V-ATPase in human diseases: Therapeutic challe...
Cátia S. Pereira; Rodrigues, L. R.; Côrte-Real, ManuelaThe control of the intracellular pH is vital for the survival of all organisms. Membrane transporters, both at the plasma and intracellular membranes, are key players in maintaining a finely tuned pH balance between intra- and extracellular spaces, and therefore in cellular homeostasis. V-ATPase is a housekeeping ATP-driven proton pump highly conserved among prokaryotes and eukaryotes. This proton pump, which e...
Deciphering the interaction of lactoferrin with V-ATPase towardsa deeper unders...
Cátia S. Pereira; Rocha, Juliana F.; Fernandes, Henrique S.; Rodrigues, L. R.; Côrte-Real, Manuela; Sousa, Sérgio F.Lactoferrin (Lf), a bioactive milk protein, exhibits strong anticancer and antifungal activities. The search for Lf targets and mechanisms of action is of utmost importance to enhance its effective applications. A common feature among Lf-treated cancer and fungal cells is the inhibition of a proton pump called V-ATPase. Lf-driven V-ATPase inhibition leads to cytosolic acidification, ultimately causing cell deat...
Lactoferrin selectively triggers apoptosis in highly metastatic breast cancer c...
Cátia S. Pereira; Guedes, J.; Gonçalves, Marília; Loureiro, Luís; Castro, Lisandra; Gerós, H.; Rodrigues, L. R.; Côrte-Real, ManuelaBreast cancer is the most common cancer in women worldwide. Metastatic breast cancer cells are known to display V-H+-ATPase at the plasma membrane, where it is involved in the acquisition of a more metastatic phenotype. Therefore, V-H+-ATPase emerged as a promising candidate for breast cancer therapy, and inhibitors of this pump as potential anticancer drugs. Lactoferrin (Lf) is a natural pro-apoptotic glycopro...