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Impact of storage time and temperature on volatomic signature of Tinta Negra wi...
Perestrelo, R.; Silva, Catarina L.; Silva, Pedro; Câmara, J. S.The current study reports the effect of storage temperature, storage time and glucose content on the volatomic signature of Tinta Negra wines using liquid-liquid microextraction (LLME) combined with gas chromatography-ion trap mass spectrometry (GC-ITMS). A total of 65 volatile organic compounds (VOCs) were identified in Tinta Negra, of which only 14 appear during storage. Based on the results, the freshness an...
Global volatile profile of virgin olive oils flavoured by aromatic/medicinal pl...
Perestrelo, R.; Silva, C.; Silva, P.; Câmara, J. S.The global volatile profile of commercial virgin olive oils and flavoured olive oils with aromatic/medicinal plants, was established using liquid-liquid microextraction (LLME) and headspace solid-phase microextraction (HS-SPME) combined with gas chromatography-quadrupole mass spectrometry (GC-qMS). More than 60 volatile organic compounds (VOCs belonging to different groups were identified using both methods. Ol...
Microextraction using packed sorbent as an effective and high-throughput sample...
Pereira, J.; Gonçalves, J.; Alves, V.; Câmara, J. S.In the last decade, several developments, particularly in the liquid chromatography and hyphenated techniques fields, have allowed researchers to reach analytical limits that are becoming very close to the single molecule level. Sample preparation, however, despite being a key step in the analytical methodology, did not track these developments and very few approaches able to cope with these stringent analytica...
Allergic asthma exhaled breath metabolome: a challenge for comprehensive two-di...
Caldeira, M.; Perestrelo, Rosa Maria de Sá; Barros, António S.; Bilelo, M. J.; Morête, A.; Câmara, J. S.; Rocha, Sílvia M.Allergic asthma represents an important public health issue, most common in the paediatric population, characterized by airway inflammation that may lead to changes in volatiles secreted via the lungs. Thus, exhaled breath has potential to be a matrix with relevant metabolomic information to characterize this disease. Progress in biochemistry, health sciences and related areas depends on instrumental advances, ...
Profiling allergic asthma volatile metabolic patterns using a headspace-solid ph...
Caldeira, M.; Barros, António S.; Bilelo, M. J.; Parada, A.; Câmara, J. S.; Rocha, Sílvia M.Allergicasthmarepresentsanimportantpublichealthissuewithsignificantgrowthovertheyears,especially in the paediatric population. Exhaled breath is a non-invasive, easily performed and rapid method forobtainingsamplesfromthelowerrespiratorytract.Inthepresentmanuscript,themetabolicvolatile profiles of allergic asthma and control children were evaluated by headspace solid-phase microextraction combined with gas chroma...
Investigation of urinary volatile organic metabolites as potential cancer bioma...
Silva, C. L.; Câmara, J. S.BACKGROUND: Non-invasive diagnostic strategies aimed at identifying biomarkers of cancer are of great interest for early cancer detection. Urine is potentially a rich source of volatile organic metabolites (VOMs) that can be used as potential cancer biomarkers. Our aim was to develop a generally reliable, rapid, sensitive, and robust analytical method for screening large numbers of urine samples, resulting in a...
Comparative analysis of the volatile fraction from Annona cherimola Mill. culti...
Ferreira, Liseth; Perestrelo, Rosa Maria de Sá; Câmara, J. S.The analysis of volatile compounds in Funchal, Madeira, Mateus and Perry Vidal cultivars of Annona cherimola Mill. (cherimoya) was carried out by headspace solid-phase microextraction (HS-SPME) combined with gas chromatography–quadrupole mass spectrometry detection (GC–qMSD). HS-SPME technique was optimized in terms of fibre selection, extraction time, extraction temperature and sample amount to reach the best ...
Potentialities of two solventless extraction approaches—Stir bar sorptive extra...
Perestrelo, Rosa Maria de Sá; Nogueira, J. M. F.; Câmara, J. S.A stir bar sorptive extraction with liquid desorption followed by large volume injection coupled to gas chromatography–quadrupole mass spectrometry (SBSE-LD/LVI-GC–qMS) was evaluated for the simultaneous determination of higher alcohol acetates (HAA), isoamyl esters (IsoE) and ethyl esters (EE) of fatty acids. The method performance was assessed and compared with other solventless technique, the solid-phase mic...
Headspace solid-phase microextraction-gas chromatography-quadrupole mass spectr...
Pontes, Marisela; Marques, J. C.; Câmara, J. S.Dynamic headspace solid-phase microextraction (HS-SPME) followed by thermal desorption gas chromatography-quadrupole mass spectrometry analysis (GC-qMS), was used to investigate the aroma profile of different species of passion fruit samples. The performance of five commercially available SPME fibres: 65 μm polydimethylsiloxane/divinylbenzene, PDMS/DVB; 100 μm polydimethylsiloxane, PDMS; 85 μm polyacrylate, PA;...
Simultaneous analysis of free amino acids and biogenic amines in honey and wine...
Pereira, Vanda Nulita Gomes; Pontes, Marisela; Câmara, J. S.; Marques, J. C.This work presents a RP-HPLC method for the simultaneous quantification of free amino acids and biogenic amines in liquid food matrices and the results of the application to honey and wine samples obtained from different production processes and geographic origins. The developed methodology is based on a pre-column derivatization with o-phthaldialdehyde carried out in the sample injection loop. The compounds we...