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Heterogeneous photocatalysis using UVA-LEDs for the removal of antibiotics and ...
Francesco Biancullo; Nuno F. F. Moreira; Ana Rita Ribeiro; Célia M. Manaia; Joaquim L. Faria; Olga C. Nunes; Sérgio M. Castro Silva; Adrián M. T. SilvaSecondary urban wastewater samples were spiked with azithromycin (AZT), trimethoprim (TMP), ofloxacin (OFL) and sulfamethoxazole (SMX) at 100 mu g L-1 to investigate the efficiency of a TiO2-photocatalytic treatment using UVA-LEDs. Different operating parameters were studied, such as the irradiation conditions, catalyst load and the use of methanol as carrier solvent and radical scavenger. The most efficient co...
Genetic variation in the conjugative plasmidome of a hospital effluent multidru...
Catarina Ferreira; Diana Bogas; Santosh K. Bikarolla; Ana Rita Varela; Karolin Frykholm; Raquel Linheiro; Olga C. Nunes; Fredrik WesterlundBacteria harboring conjugative plasmids have the potential for spreading antibiotic resistance through horizontal gene transfer. It is described that the selection and dissemination of antibiotic resistance is enhanced by stressors, like metals or antibiotics, which can occur as environmental contaminants. This study aimed at unveiling the composition of the conjugative plasmidome of a hospital effluent multidr...
Continuous ozonation of urban wastewater: Removal of antibiotics, antibiotic-re...
I. C. Iakovides; I. Michael-Kordatou; Nuno F. F. Moreira; Ana Rita Ribeiro; T. Fernandes; Manuel Fernando R. Pereira; Olga C. Nunes; Célia M. ManaiaThis work evaluated the removal of a mixture of eight antibiotics (i.e. ampicillin (AMP), azithromycin (AZM), erythromycin (ERY), clarithromycin (CIA), ofloxacin (OFL), sulfamethoxazole (SMX), trimethoprim (TMP) and tetracycline (TC)) from urban wastewater, by ozonation operated in continuous mode at different hydraulic retention times (HRTs) (i.e. 10, 20, 40 and 60 min) and specific ozone doses (i.e. 0.125, 0....
Removal of microorganisms and antibiotic resistance genes from treated urban wa...
Barbara W. N. Grehs; Ana Rita Lopes; Nuno F. F. Moreira; Telma Fernandes; Maria A.O. Linton; Adrián M.T. Silva; Célia M. Manaia; Elvis CarissimiThe presence of antibiotic resistant-bacteria (ARB) and antibiotic resistance genes (ARG) in treated effluents of urban wastewater treatment plants (WWTP) may represent a threat to the environment and public health. Therefore, cost-effective technologies contributing to minimize loads of these contaminants in the final effluents of WWTP are required. This study aimed at assessing the capacity of coagulation to ...
Solar treatment (H2O2, TiO2-P25 and GO-TiO2 photocatalysis, photo-Fenton) of or...
Nuno F. F. Moreira; Carlos Narciso da Rocha; M. Inmaculada Polo-López; Luisa M. Pastrana-Martínez; Joaquim L. Faria; Célia M. ManaiaSolar-driven advanced oxidation processes were studied in a pilot-scale photoreactor, as tertiary treatments of effluents from an urban wastewater treatment plant. Solar-H2O2, heterogeneous photocatalysis (with and/or without the addition of H2O2 and employing three different photocatalysts) and the photo-Fenton process were investigated. Chemical (sulfamethoxazole, carbamazepine, and diclofenac) and biological...
Ozonation and UV254 nm radiation for the removal of microorganisms and antibiot...
José M. Sousa; Gonçalo Macedo; Marta Pedrosa; Cristina Becerra Castro; Sérgio Castro Silva; M. Fernando R. Pereira; Adrián M. T. Silva; Olga C. NunesConventional wastewater treatment has a limited capacity to reduce antibiotic resistant bacteria and genes (ARB&ARG). Tertiary treatment processes are promising solutions, although the transitory inactivation of bacteria may select ARB&ARG. This study aimed at assessing the potential of ozonation and UV254 nm radiation to inactivate cultivable fungal and bacterial populations, and the selected genes 16S rRNA (c...
Microbial diversity and ecology of bottled water
Célia M. Manaia; Olga C. Nunes"Bottled water" refers to natural or treated water that reaches the consumer as a packaged product, normally a glass or plastic container. Mineral or spring waters that do not suffer any treatment capable of altering their natural microbiota are appreciated by consumers and constitute interesting microbial habitats. These types of water have a rich and diversified microbiota, which despite the variations that m...
Ubiquitous and persistent Proteobacteria and other Gram-negative bacteria in dr...
Ivone Vaz Moreira; Olga C. Nunes; Célia M. ManaiaDrinking water comprises a complex microbiota, in part shaped by the disinfection and distribution systems. Gram-negative bacteria, mainly members of the phylum Proteobacteria, represent the most frequent bacteria in drinking water, and their ubiquity and physiological versatility raises questions about possible implications in human health. The first step to address this concern is the identification and chara...
Characterization of bacterial communities from Masseiras, a unique Portuguese g...
Cristina Becerra Castro; Ana Rita Lopes; Sara Teixeira; M. Elisabete F. Silva; Elisabete Pimenta; Célia M. Manaia; Olga C. Nunes"Masseiras" is an ancient Portuguese agriculture system, where soil was developed from sand dunes enriched with seaweeds over more than a century. Due to the importance for the local economy, this system evolved for greenhouse structures. In this study we compared the bacterial community composition and structure of Masseiras soil, aiming at assessing the potential impact of different agricultural practices. Th...
Photocatalytic ozonation of urban wastewater and surface water using immobilize...
Nuno F. F. Moreira; José M. Sousa; Gonçalo Macedo; Ana Rita Ribeiro; Luisa Barreiros; Marta Pedrosa; Joaquim L. Faria; Manuel Fernando R. PereiraPhotocatalytic ozonation was employed for the first time in continuous mode with TiO2-coated glass Raschig rings and light emitting diodes (LEDs) to treat urban wastewater as well as surface water collected from the supply area of a drinking water treatment plant (DWTP). Different levels of contamination and types of contaminants were considered in this work, including chemical priority substances (PSs) and con...