129 documents found, page 1 of 13
Understanding lactoferrin multifunctionality by analyzing the full spectrum of ...
Ferreira, Sofia; Guedes, Joana P.; Rocha, Juliana F.; Sousa, Sérgio F.; Rodrigues, L. R.; Côrte-Real, Manuela; Soares, Pedro; Cátia S. PereiraLactoferrin (LF) is a multifunctional protein involved in critical biological processes such as immune response, antimicrobial activity, and iron regulation [1]. Its diverse roles include inhibiting inflammation, infections, and carcinogenesis [2], as well as modulating cellular processes by targeting proton-pumping ATPases such as V-ATPases in cancer cells [3], F- ATPases in bacteria [4], and P-ATPases in yeas...
Current and novel approaches in yeast cell death research
Chaves, Susana Alexandra Rodrigues; Rego, António; Cátia S. Pereira; Sousa, Maria João; Côrte-Real, ManuelaThe study of cell death mechanisms in fungi, particularly yeasts, has gained substantial interest in recent decades driven by the potential for biotechnological advancements and therapeutic interventions. Examples include the development of robust yeast strains for industrial fermentations and high-value compound production, novel food preservation strategies against spoilage yeasts, and the identification of t...
Enhancement of acetate-induced apoptosis of colorectal cancer cells by cathepsi...
Alves, Sara; Santos-Pereira, Cátia; Oliveira, Cláudia S. F.; Preto, Ana; Chaves, Susana R.; Côrte-Real, ManuelaColorectal cancer (CRC) is a leading cause of death worldwide. Conventional therapies are available with varying effectiveness. Acetate, a short-chain fatty acid produced by human intestinal bacteria, triggers mitochondria-mediated apoptosis preferentially in CRC but not in normal colonocytes, which has spurred an interest in its use for CRC prevention/therapy. We previously uncovered that acetate-induced mitoc...
Citrate-coated manganese and calcium ferrite nanoparticles as potential magneti...
Andrade, Raquel G. D.; Ferreira, Débora Carina Gonçalves Abreu; Veloso, Sérgio Rafael Silva; Santos-Pereira, Cátia; Castanheira, Elisabete M. S.In this work, manganese/calcium ferrite NPs coated with citrate, were synthesized by the sol-gel method, followed by calcination, and then characterized regarding their crystalline structure (by X-ray diffraction, XRD), size and shape (by Transmission Electron Microscopy, TEM), hydrodynamic size and zeta potential (by Dynamic Light Scattering, DLS), and heating efficiency (measuring the Specific Absorption Rate...
A versatile yeast model identifies the pesticides cymoxanil and metalaxyl as ri...
Amaral, Leslie Ann Silva; Mendes, Filipa; Côrte-Real, Manuela; Rego, António MIguel Araújo; Outeiro, Tiago F.; Chaves, Susana R.Parkinson's disease (PD) is a neurodegenerative disorder characterized by the loss of dopaminergic neurons and the presence of Lewy bodies, which predominantly consist of aggregated forms of the protein alpha-synuclein (aSyn). While these aggregates are a pathological hallmark of PD, the etiology of most cases remains elusive. Although environmental risk factors have been identified, such as the pesticides diel...
Bovine lactoferrin-loaded plasmonic magnetoliposomes for antifungal therapeutic...
Pereira, Mélanie; Rodrigues, Ana Rita Oliveira; Amaral, Leslie; Côrte-Real, Manuela; Pereira, Cátia Sofia Santos; Castanheira, Elisabete M. S.Bovine lactoferrin (bLf) is a milk-derived protein that exhibits potent broad-spectrum antifungal activity against multiple fungi. bLf is susceptible to degradation, while some of its properties depend on the tertiary structure. So, the encapsulation of bLf in stimuli-responsive therapeutic formulations provides an added value to enhance its biological activities. Plasmonic magnetoliposomes (PMLs) arise as prom...
Saccharomyces cerevisiae cells lacking the zinc vacuolar transporter Zrt3 displ...
Terra-Matos, Joana; Teixeira, Marta Oliveira; Pereira, Cátia Sofia Santos; Noronha, Henrique; Domingues, Lucília; Sieiro, Carmen; Gerós, H.Yeast-based bioethanol production from lignocellulosic hydrolysates (LH) is an attractive and sustainable alternative for biofuel production. However, the presence of acetic acid (AA) in LH is still a major problem. Indeed, above certain concentrations, AA inhibits yeast fermentation and triggers a regulated cell death (RCD) process mediated by the mitochondria and vacuole. Understanding the mechanisms involved...
Plasmalemmal V-ATPase as a potential biomarker for lactoferrin-based anticancer...
Pereira, Cátia Sofia Santos; Rodrigues, L. R.; Côrte-Real, ManuelaLactoferrin (Lf) is a milk-derived protein with well-recognized potential as a therapeutic agent against a wide variety of cancers. This natural protein exhibits health-promoting effects and has several interesting features, including its selectivity towards cancer cells, good tolerability in humans, worldwide availability, and holding a generally recognized as safe (GRAS) status. To prompt the rational clinica...
A review on lactoferrin as a proton pump inhibitor
Pereira, Cátia Sofia Santos; Andrés, María T.; Fierro, José F.; Rodrigues, L. R.; Côrte-Real, ManuelaLactoferrin (Lf) is a versatile natural milk-derived protein that exhibits multiple interesting biological activities. Since it is safe for human administration and currently manufactured using low cost and well-established large-scale processes, the Lf scientific community has been devoted at dissecting its mechanisms of action towards its more rational and efficient use for various applications. Emerging lite...
Lactoferrin perturbs intracellular trafficking, disrupts cholesterol-rich lipid...
Pereira, Cátia Sofia Santos; Guedes, Joana Catarina Pereira; Ferreira, Débora Carina Gonçalves Abreu; Rodrigues, L. R.; Côrte-Real, ManuelaThe milk-derived bovine lactoferrin (bLf) is an iron-binding glycoprotein with remarkable selective anticancer activity towards highly metastatic cancer cells displaying the proton pump V-ATPase at the plasma membrane. As studies aiming to dissect the bLf mechanisms of action are critical to improve its efficacy and boost its targeted clinical use, herein we sought to further uncover the molecular basis of bLf ...