37 documents found, page 1 of 4
MicroVasculoid-chip: a 3D self-assembled human microcirculation-on-a-chip model...
Abreu, Catarina M.; Lima, Ana Cláudia Fernandes; Neves, N. M.; Kundu, Subhas C; Reis, R. L.; Caballero, DavidThe microvasculature within the tumor microenvironment is crucial for the invasion and dissemination of cancer cells throughout the body. Given its importance and dynamic behavior, several microfluidic models have been developed to study microvascular infiltration and its interaction with cancer cells. However, most of these models primarily focus on blood vessels and use microfluidic channels coated with endot...
Patterned glycopeptide-based supramolecular hydrogel promotes the alignment and...
Castro, Vânia Isabel Baptista; Amorim, Sara; Caballero, David; Abreu, Catarina M.; Kundu, Subhas C; Reis, R. L.; Pashkuleva, I.; Pires, R. A.The functional restoration of a damaged cardiac tissue relies on a synchronized contractile capacity of exogenous and/or endogenous cardiomyocytes, which is challenging to achieve. Here, we explored the potential of the short glycopeptide diphenylalanine glucosamine-6-sulfate (FFGlcN6S) conjugated with an aromatic moiety, namely fluorenylmethoxycarbonyl (Fmoc), to enhance cardiac tissue regeneration. At physiol...
Evaluation of novel dendrimer-gold complex nanoparticles for theranostic applic...
Milivojevic, Nevena; Carvalho, Mariana Rodrigues; Caballero, David; Radisavljevic, Snezana; Radoicic, Marija; Živanović, Marko; Kundu, Subhas CAim: Despite some successful examples of therapeutic nanoparticles reaching clinical stages, there is still a significant need for novel formulations in order to improve the selectivity and efficacy of cancer treatment. Methods: The authors developed two novel dendrimerâ gold (Au) complex-based nanoparticles using twodifferent synthesis routes: complexation method (formulation A) and precipitation method (formu...
Human tendon-on-chip: unveiling the effect of core compartment-t cell spatiotem...
Bakht, Syeda M.; Pardo, Alberto; Gómez-Florit, Manuel; Caballero, David; Kundu, Subhas C; Reis, R. L.; Domingues, Rui Miguel Andrade; Gomes, Manuela E.The lack of representative in vitro models recapitulating human tendon (patho)physiology is among the major factors hindering consistent progress in the knowledge-based development of adequate therapies for tendinopathy.Here, an organotypic 3D tendon-on-chip model is designed that allows studying the spatiotemporal dynamics of its cellular and molecular mechanisms.Combining the synergistic effects of a bioactiv...
Biomimetic and soft lab-on-a-chip platform based on enzymatic-crosslinked silk ...
Carvalho, M. R.; Caballero, David; Subhas K. C.; Reis, R. L.Integrating biological material within soft microfluidic systems made of hydrogels offers countless possibilities in biomedical research to overcome the intrinsic limitations of traditional microfluidics based on solid, non-biodegradable, and non-biocompatible materials. Hydrogel-based microfluidic technologies have the potential to transform in vitro cell/tissue culture and modeling. However, most hydrogel-bas...
Fibroblasts Impair Migration and Antitumor Activity of NK-92 Lymphocytes in a M...
Iaia, Ilenia; Brancato, Virginia; Caballero, David; Reis, R. L.; Aglietta, Massimo; Sangiolo, Dario; Kundu, Subhas CAdoptive cell therapy in solid tumors, such as melanoma, is impaired, but little is known about the role that the fibroblasts present in the tumor microenvironment could exert. However, the mechanism at play is not well understood, partly due to the lack of relevant pre-clinical models. Three-dimensional culture and microfluidic chips are used to recapitulate the dynamic interactions among different types of ce...
Microfluidic-assisted interfacial complexation of extracellular matrix componen...
Costa, R. R.; Caballero, David; Soares da Costa, Diana; Rodriguez-Trujillo, R.; Kundu, Subhas C; Reis, R. L.; Pashkuleva, I.Anisotropy is an important cue for neural organization during morphogenesis and healing, contributing to the mechanical and functional properties of neural tissues. The ability to replicate such anisotropy in vitro holds great promise for the development of effective regeneration strategies. In this work, interfacial polyelectrolyte complexation (IPC) is applied to fabricate microfibers from charged ECM compone...
Cancer traps: implantable and on-chip solutions for early cancer detection and ...
Caballero, David; Reis, R. L.; Kundu, Subhas CCancer continues to be a major global health issue causing millions of deaths annually. While traditional therapeutic methods may be effective in many cases, they may not be suitable for highly metastatic cancers. Moreover, the late detection of tumors, when they have already spread and are harder to treat, further exacerbates the challenge in managing this disease. As a result, there is a growing interest in d...
Assessing cell migration in hydrogels: an overview of relevant materials and me...
Solbu, A. A.; Caballero, David; Damigos, S.; Kundu, Subhas C; Reis, R. L.; Halaas, Ø.; Chahal, A. S.; Berit, L. S.Cell migration is essential in numerous living processes, including embryonic development, wound healing, immune responses, and cancer metastasis. From individual cells to collectively migrating epithelial sheets, the locomotion of cells is tightly regulated by multiple structural, chemical, and biological factors. However, the high complexity of this process limits the understanding of the influence of each fa...
Trapping metastatic cancer cells with mechanical ratchet arrays
Caballero, David; Reis, R. L.; Kundu, Subhas CCurrent treatments for cancer, such as chemotherapy, radiotherapy, immunotherapy, and surgery, have positive results but are generally ineffective against metastatic tumors. Treatment effectiveness can be improved by employing bioengineered cancer traps, typically utilizing chemoattractant-loaded materials, to attract infiltrating cancer cells preventing their uncontrolled spread and potentially enabling eradic...