5 documents found, page 1 of 1
3D bioprinting of novel κ-carrageenan bioinks : an algae-derived polysaccharide
Marques, Diana M. C.; Silva, João C.; Serro, Ana Paula; Cabral, Joaquim M. S.; Sanjuan-Alberte, Paola; Ferreira, Frederico C.Novel green materials not sourced from animals and with low environmental impact are becoming increasingly appealing for biomedical and cellular agriculture applications. Marine biomaterials are a rich source of structurally diverse compounds with various biological activities. Kappa-carrageenan (κ-c) is a potential candidate for tissue engineering applications due to its gelation properties, mechanical strengt...
Effects of glycosaminoglycan supplementation in the chondrogenic differentiatio...
Silva, João C.; Moura, Carla; Matos, António P. Alves de; Cabral, Joaquim M. S.; Linhardt, Robert J.; Ferreira, Frederico CasteloThe lack of effective and long-term treatments for articular cartilage defects has increased the interest for innovative tissue engineering strategies. Such approaches, combining cells, biomaterial matrices and external biochemical/physical cues, hold promise for generating fully functional cartilage tissue. Herein, this study aims at exploring the use of the major cartilage glycosaminoglycans (GAGs), chondroit...
Extruded bioreactor perfusion culture supports the chondrogenic differentiation...
Silva, João C.; Moura, Carla; Borrecho, Gonçalo; Matos, António P. Alves de; Silva, Cláudia L. da; Cabral, Joaquim M. S.; Bártolo, Paulo J.Novel bioengineering strategies for the ex vivo fabrication of native-like tissue-engineered cartilage are crucial for the translation of these approaches to clinically manage highly prevalent and debilitating joint diseases. Bioreactors that provide different biophysical stimuli have been used in tissue engineering approaches aimed at enhancing the quality of the cartilage tissue generated. However, such syste...
Extracellular matrix decorated polycaprolactone scaffolds for improved mesenchy...
Silva, João C.; Carvalho, Marta S.; Udangawa, Ranodhi N.; Moura, Carla; Cabral, Joaquim M. S.; Silva, Cláudia L. da; Ferreira, Frederico CasteloThe clinical demand for tissue-engineered bone is growing due to the increase of non-union fractures and delayed healing in an aging population. Herein, we present a method combining additive manufacturing (AM) techniques with cell-derived extracellular matrix (ECM) to generate structurally well-defined bioactive scaffolds for bone tissue engineering (BTE). In this work, highly porous three-dimensional polycapr...
Developing a co-culture system for effective megakaryo/thrombopoiesis from umbi...
Hatami, Javad; Andrade, Pedro Z.; Alves de Matos, António Pedro; Djokovic, Dusan; Lilaia, Carla; Ferreira, Frederico Castelo; Cabral, Joaquim M. S.Background aims. Platelet transfusion can be a life-saving procedure in different medical settings. Thus, there is an increasing demand for platelets, of which shelf-life is only 5 days. The efficient ex vivo biomanufacturing of platelets would allow overcoming the shortages of donated platelets. Methods. We exploited a two-stage culture protocol aiming to study the effect of different parameters on the megakar...