3 documents found, page 1 of 1
Attomolar detection of hepatitis C virus core protein powered by molecular ante...
Palacio, Irene; Moreno, Miguel; Náñez, Almudena; Purwidyantri, Agnes; Domingues, Telma; Cabral, Patrícia D.; Borme, Jérôme; Marciello, MarziaBiosensors based on graphene field-effect transistors have become a promising tool for detecting a broad range of analytes. However, their performance is substantially affected by the functionalization protocol. In this work, we use a controlled in-vacuum physical method for the covalent functionalization of graphene to construct ultrasensitive aptamer-based biosensors (aptasensors) able to detect hepatitis C v...
Clean-Room lithographical processes for the fabrication of Graphene biosensors
Cabral, Patrícia D.; Domingues, Telma; Machado, George; Chicharo, Alexandre; Cerqueira, M. F.; Fernandes, Elisabete; Athayde, Emilia; Alpuim, P.This work is on developing clean-room processes for the fabrication of electrolyte-gate graphene field-effect transistors at the wafer scale for biosensing applications. Our fabrication process overcomes two main issues: removing surface residues after graphene patterning and the dielectric passivation of metallic contacts. A graphene residue-free transfer process is achieved by using a pre-transfer, sacrificia...
Functionalization of single-layer graphene for immunoassays
Fernandes, Elisabete; Cabral, Patrícia D.; Campos, Rui; Machado, George; Cerqueira, M. F.; Sousa, Cláudia; Freitas, Paulo P.; Borme, JérômeThe excellent electronic properties of single-layer graphene provide the fundamental basis for its use in advanced electronics applications. Graphene-based biosensors, however, are one of the exceptions among the applications that take advantage of the unique properties of this material, because high hydrophobicity of graphene hinders the controlled immobilization of biomolecular probes, particularly of antibod...