7 documents found, page 1 of 1
Padronização de método de concentração e extração de ácidos nucleicos em amostr...
Cabral,Aline Diniz; Claro,Ieda Carolina Mantovani; Augusto,Matheus Ribeiro; Friolani,Veronica Nikoluk; Bezerra,Cintia de EspindolaRESUMO A vigilância da qualidade dos esgotos sanitários pode representar uma ferramenta complementar para monitoramento de doenças infecciosas e prevenção de surtos epidêmicos, especialmente quando a capacidade para testes clínicos é limitada. Dessa maneira, o presente estudo descreve o detalhamento técnico de um método de baixo custo para a concentração e extração de ácidos nucleicos de amostras de esgoto sani...
Zika virus serological diagnosis: commercial tests and monoclonal antibodies as...
Silva,Isaura Beatriz Borges; Silva,Aldacilene Souza da; Cunha,Mariana Sequetin; Cabral,Aline Diniz; Oliveira,Kelly Cristina Alves deAbstract Zika virus (ZIKV), an emerging arthropod-borne virus (arbovirus) of the Flaviviridae family, is a current issue worldwide, particularly because of the congenital and neurological syndromes associated with infection by this virus. As the initial clinical symptoms of all diseases caused by this group are very similar, clinical diagnosis is difficult. Furthermore, laboratory diagnostic efforts have failed...
Prevalence of antibodies against Neospora spp. and Sarcocystis neurona in donke...
Gennari,Solange Maria; Pena,Hilda Fátima de Jesus; Lindsay,David Scott; Lopes,Marcos Gomes; Soares,Herbert Sousa; Cabral,Aline DinizAbstract Sarcocystis neurona and Neospora hughesi are coccidian protozoa that can cause neurological illness in horses in America. In this study we report seroprevalence of Neospora spp. andS. neurona in sera of 333 donkeys from the northeastern region of Brazil. Antibodies to Neospora spp. were detected in 2% (7 donkeys) of 333 sera tested by the indirect fluorescent antibody test (IFAT) with a cut-off dilutio...
Toxoplasma gondii in domestic and wild animals from forest fragments of the mun...
Fournier,Gislene Fátima da Silva Rocha; Lopes,Marcos Gomes; Marcili,Arlei; Ramirez,Diego Garcia; Acosta,Igor Cunha LimaToxoplasmosis stands out as a global disease that has felines as definitive hosts. In the municipality of Natal, Rio Grande do Norte State, Brazil, two parks are notable for their ecological and social importance. This study aimed to investigate the presence of Toxoplasma gondii in short hair cats, bats and small non-volant mammals in these two ecological reserves. Altogether, biological samples were obtained f...
Frequência de ocorrência de Giardia sp. e de Cryptosporidium spp. em equídeos n...
Nasciutti,Nayara Resende; Cabral,Dagmar Diniz; Cabral,Aline Diniz; Souza,Rafael Rocha de; Oliveira,Patricia Magalhães deDentre os protozoários de maior importância em saúde pública estão Cryptosporidium spp. e Giardia sp. pelo potencial zoonótico. Pesquisou-se a frequência de ocorrência desses parasitas em equídeos abatidos em um frigorífico no município de Araguari, Minas Gerais, durante o período de fevereiro a março de 2008, correlacionando com o sexo, espécie e procedência dos animais. Foram coletadas 150 amostras de fezes, ...
Neospora caninum as causative agent of bovine encephalitis in Brazil
Malaguti,Jane Mary Albinati; Cabral,Aline Diniz; Abdalla,Raisa Pereira; Salgueiro,Yolanda Oliveira; Galleti,Nara Thiers Cacciatori; Okuda,Liria HiromiFor supporting the Brazilian bovine encephalitis surveillance program this study examined the differential diagnosis of Neospora caninum in central nervous system (CNS) by histological analysis (HE staining), immunohistochemistry (IHC), and nested-PCR using a set of primers from the Nc5 region of the genomic DNA and ITS1 region of the ribosomal DNA. A sample of 302 cattle presenting neurological syndrome and ne...
Diagnosis of Neospora caninum in bovine fetuses by histology, immunohistochemis...
Cabral,Aline Diniz; Camargo,Clarice Neves; Galleti,Nara Thiers Cacciatori; Okuda,Liria Hiromi; Pituco,Edviges Maristela; Del Fava,ClaudiaNeospora caninum, a cause of abortion and stillbirth in cattle, was studied by histology, immunohistochemistry, and nested-PCR, using primers from the Nc5 region of the genomic DNA (PCR PLUS) and primers from the ITS1 region of the ribosomal DNA (PCR JB). A total of 105 fetal samples sent to the Centro de Pesquisa e Desenvolvimento de Sanidade Animal do Instituto Biológico from January 2006 to May 2008 were exa...