49 documents found, page 1 of 5
Testis transcriptomic analyses reveal the effects of an algae feed on sperm qua...
Félix de Azeredo Pinto e Melo, Francisca; Raposo de Magalhães, Cláudia; Marrero Alemán, Carlos; Duarte, Daniel Filipe Correia; Parente, PedroThe usage of dietary algae antioxidants to improve fish reproduction is under-explored, especially in terms of the male reproductive system. In this experiment, 6 % of a blended meal of Phaeodactylum tricornutum and Gracilaria gracilis was incorporated in Senegalese sole broodstock feed, to evaluate the effects on sperm quality of F1 males throughout the breeding season. For that, two groups of breeders were fe...
The use of egg quality parameters to evaluate the effect of a diet supplemented...
Ramos Júdez, Sandra; Fatsini Fernández, Elvira; Marrero Alemán, Carlos; García-Pichel, Candela; Parente, Pedro; Medina, D.; Castro, C.; Cabrita, ElsaEnhancing egg quality can be achieved by improving breeders’ diet through the antioxidant content, as oxidative stress could adversely affect egg quality. Micro- and macroalgae species are natural sources of antioxidants and other essential nutrients that can be incorporated in fish feeds. In this study the quality of Scophthalmus maximus eggs were compared between breeders fed a commercial (non-supplemented) d...
Melatonin rhythms beyond the pineal organ: gene expression of receptors and bio...
Félix de Azeredo Pinto e Melo, Francisca; Oliveira, Catarina; Martín, Ignacio; Manchado, Manuel; Sánchez-Vázquez, Francisco J.; Vera, Luisa M.Fish gonadal melatonin production is still unexplored and could contribute to a better understanding of its role in reproduction control, especially for species with reproductive impairments. This study aimed to comprehend if Senegalese sole testes are an extra-pineal production site of melatonin and if it has seasonal and daily variations. Wild and F1 broodstocks were sampled in the breeding season (BS) and ou...
Cryopreservation did not affect spermatogonia global methylation profile in Sen...
Almeida, Maria Mafalda; Cabrita, Elsa; Laizé, Vincent; Brionne, Aurélien; Labbé, Catherine; Fatsini Fernández, ElviraSpermatogonia cryopreservation is a method to preserve valuable genomes from both maternal and paternal origin. The damage associated with the application of this technology on post-thaw cell quality is important to assess, including at the epigenetic level. This study aimed to assess post-thawed spermatogonia quality by evaluating alterations in plasma membrane integrity, DNA integrity (fragmentation and apopt...
Comparative transcriptome analysis reveals molecular damage associated with cry...
Anjos, Catarina; Duarte, Daniel Filipe Correia; Fatsini Fernández, Elvira; Domitília Matias; Cabrita, ElsaBackground The Portuguese oyster Crassostrea angulata, a bivalve of significant economic and ecological importance, has faced a decline in both production and natural populations due to pathologies, climate change, and anthropogenic factors. To safeguard its genetic diversity and improve reproductive management, cryopreservation emerges as a valuable strategy. However, the cryopreservation methodologies lead to...
Melatonin production improves Senegalese sole sperm motility at night, but fail...
Félix de Azeredo Pinto e Melo, Francisca; Ferrão, Leonor; Gallego, Victor; Oliveira, Catarina; Cabrita, ElsaMelatonin is a powerful antioxidant present in fish seminal plasma. This study aimed to understand melatonin's endogenous and exogenous effects on first-generation Senegalese sole sperm quality for sperm management applications. In the first experiment, samples were collected at mid-light (ML) and mid-dark (MD) daytimes, to evaluate the effects on sperm motility. In a second experiment, using confocal microscop...
Cutting-Edge methods in Teleost and Chondrichthyan reproductive biology
Mechaly, Alejandro S.; Awruch, Cynthia; Cabrita, Elsa; Costas, Benjamín; Fernandes, Jorge M.O.; Gallego, Victor; Hirt-Chabbert, JorgeReproduction is a fundamental biological process for producing offspring and ensuring the continuation of the species. With nearly 35,000 fish species worldwide exhibiting a very diverse range of reproductive systems and strategies, the study of fish reproduction is a significant challenge. Research into various fish reproductive models is driven by their use in human biomedical applications, improving the dive...
Histological evaluation of purple sea urchin (Paracentrotus lividus) gonads: in...
Araújo, João; Gamboa, Margarida; Choulis, Ioannis; Mendes, Ana Candeias; Cabrita, Elsa; Pousão-Ferreira, Pedro; Soares, FlorbelaUnderstanding and controlling reproductive cycles and gonad maturation in cultivated species are crucial in aquaculture. The sea urchin Paracentrotus lividus, known for its edible gonads, requires careful maturation control for both reproduction and commercialization. This study explores the impact of temperature, photoperiod, and diet on gonad development to enhance year-round aquaculture practices. Using two ...
Effects of dietary supplementation with macroalgae on sperm quality and antioxi...
Félix de Azeredo Pinto e Melo, Francisca; Silva, Nádia; Oliveira, Catarina; Cabrita, Elsa; Gavaia, PauloWithin the efforts in the search for novel feed ingredients in aquaculture, macroalgae are being widely explored due to their diversity of bioactive compounds with considerable prophylactic and therapeutic potential. Plocamium cartilagineum and Sargassum vulgare, have the potential to positively impact fish health, mainly due to their antioxidant properties. The production of Senegalese sole (Solea senegalensis...
Comparative transcriptome analysis reveals molecular damage associated with cry...
Anjos, Catarina; Duarte, Daniel Filipe Correia; Fatsini Fernández, Elvira; Matias, Domitília; Cabrita, ElsaThe Portuguese oyster Crassostrea angulata, a bivalve of significant economic and ecological importance, has faced a decline in both production and natural populations due to pathologies, climate change, and anthropogenic factors. To safeguard its genetic diversity and improve reproductive management, cryopreservation emerges as a valuable strategy. However, the cryopreservation methodologies lead to some damag...