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Towards the definition of an antibiotic resistome signature in wastewater and d...

Cachetas, Diogo Macedo; Vaz-Moreira, Ivone; Pereira, Vítor; Manaia, Célia M.

Domestic wastewater is a significant reservoir of antibiotic resistance genes, which pose environmental and public health risks. We aimed to define an antibiotic resistome signature, represented by core genes, i.e., shared by 90% of the metagenomes of each of three conceptual environmental compartments wastewater (influent, sludge, effluent), freshwater, and agricultural soil. The definition of resistome signat...


Microplastics biodegradation by estuarine and landfill microbiomes

Pires, Cristina S.; Costa, Luís; Barbosa, Sónia G.; Sequeira, João Carlos; Cachetas, Diogo Macedo; Freitas, José; Martins, Gilberto; Machado, Ana Vera

Plastic pollution poses a worldwide environmental challenge, affecting wildlife and human health. Assessing the biodegradation capabilities of natural microbiomes in environments contaminated with microplastics is crucial for mitigating the effects of plastic pollution. In this work, we evaluated the potential of landfill leachate (LL) and estuarine sediments (ES) to biodegrade polyethylene (PE), polyethylene t...


Metagenomic wastewater and freshwater core resistome analysis

Cachetas, Diogo Macedo

A escassez de água é atualmente uma grande preocupação. A reutilização de águas residuais tratadas, seja por descarga em ambientes hídricos (por exemplo, rios) ou pela utilização em irrigação, é apontada como umas das principais soluções. No entanto, é importante monitorizar o possível impacto desta reutilização, sobretudo ao nível da disseminação de contaminantes emergentes como as bactérias resistentes a anti...


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