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Serum and saliva immunoglobulin (immunoglobulin G, immunoglobulin A, and immuno...

Silva, Flávio; Lamy, Elsa; Conceição, Cristina; Cerqueira, Joaquim; Ramalho, Joana; González-Cabrera, Marta; Caetano, Pedro; Martins, Luís

The transfer of passive immunity is essential to ensure the health and welfare of new-born calves. Although the dynamics of serum Ig concentrations in these animals are well-described, data about saliva Ig are limited. This study aimed to evaluate serum and saliva Ig (i.e., IgG, IgA, and IgM) concentrations during the first week of life in healthy and sick calves. Blood and saliva samples were collected from 20...


Genome-wide association study identifies genetic variants underlying footrot in...

Gaspar, Daniel; Ginja, Catarina; Carolino, Nuno; Leão, Célia; Monteiro, Helena; Tábuas, Lino; Branco, Sandra; Padre, Ludovina; Caetano, Pedro

Ovine footrot caused by Dichelobacter nodosus (D. nodosus) is a contagious disease with serious economic and welfare impacts in sheep production systems worldwide. A better understanding of the host genetic architecture regarding footrot resistance/susceptibility is crucial to develop disease control strategies that efficiently reduce infection and its severity. A genome-wide association study was performed usi...


A importância da Biossegurança em explorações de bovinos de carne

Caetano, Pedro; Silva, Flávio

Os autores referem a importância das medidas de biossegurança em explorações de bovinos de carne. Atualmente a maioria dos produtores responsáveis por estas explorações não se encontram devidamente sensibilizados para essa questão, apesar do enorme impacto económico que a mesma acarreta. São referidas diversas medidas que devem fazer parte de um programa de biossegurança, enfatizando-se a necessidade de definir...


Genome-wide association study identifies genetic variants underlying footrot in...

Gaspar, Daniel; Ginja, Catarina; Carolino, Nuno; Leão, Célia; Monteiro, Helena; Tábuas, Lino; Branco, Sandra; Padre, Ludovina; Caetano, Pedro

This is the first attempt to identify molecular markers associated with footrot in Portuguese Merino sheep. These findings provide relevant information on a likely genetic association underlying footrot resistance/susceptibility and the potential candidate genes affecting this trait. Genetic selection strategies assisted on the information obtained from this study could enhance Merino sheep-breeding programs, i...


A metagenomics approach to characterize the footrot microbiome in Merino sheep

Usié, Ana; Leão, Célia; Gaspar, Daniel; Monteiro, Helena; Tábuas, Lino; Bettencourt, Elisa; Caetano, Pedro; Padre, Ludovina; Carolino, Nuno

In the Portuguese Alentejo region, Merino sheep breed is the most common breed, reared for the production of meat, dairy, and wool. Footrot is responsible for lameness, decreased animal welfare, and higher production losses, generating a negative economic impact. The disease is caused by Dichelobacter nodosus that interacts with the sheep foot microbiome, to date largely uncharacterized. In fact, Dichelobacter ...


Evaluation of the birth impact in the newborn dairy calf using ethophysiologica...

Silva, Flávio; Pedro, Sofia; Azevedo, Inês; Ramalho, Joana; Caetano, Pedro; Martins, Luís; Cerqueira, Joaquim; Silva, Severiano; Conceição, Cristina

The calving moment is one of the most stressful events in the calf’s life. Therefore, evaluating the level of the birth impact in the calf can provide valuable information for both nursery and research activities. The evaluation can be performed by measuring calf’s vitality, or vigor, using ethologic and physiological parameters. However, there is a lack of general agreement regarding which measures are more su...


The relationship between calving, colostrum management and passive immune transfer

Silva, Flávio; Conceição, Cristina; Cerqueira, Joaquim; Pedro, Sofia; Azevedo, Inês; Ramalho, Joana; Caetano, Pedro; Pereira, Alfredo; Martins, Luís

Failure of passive immune transfer is a widespread problem in dairy farming. Recent scientific recommendations and welfare requisites indicate that passive immune transfer (PIT) should not be treated as a binary variable but as a continuous variable. In other words, the calf benefits from the best immunization possible. So, the objective of this study was to investigate the management factors associated with th...


Validation study of the optical refractometry method to measure total proteins ...

Silva, Flávio; Martins, Luís; Azevedo, Inês; Pedro, Sofia; Ramalho, Joana; Caetano, Pedro; Cerqueira, Joaquim; Conceição, Cristina; Silva, Severiano

Failure of passive immune transfer is a condition attributed to calves that did not receive an appropriated doses of immunoglobulins through colostrum, which is known to compromise calf’s health and welfare, usually leading to an increase in antimicrobials’ usage. At the farm level, immunoglobulins analysis is not feasible, thus, the standard method consists in measuring the total protein concentration in serum...


Avaliação da qualidade do colostro: uma ferramenta para aumentar a eficiência n...

Silva, Flávio; Cerqueira, Joaquim; Silva, Severiano; Ramalho, Joana; Caetano, Pedro; Martins, Luís; Pereira, Alfredo; Conceição, Cristina

O colostro é para as espécies ruminantes um alimento essencial nas primeiras horas de vida. A sua ingestão confere ao neonato um suporte energético e imunológico determinante para os processos biológicos relacionados com a termogénese, com o desenvolvimento gastrointestinal e com a transferência de imunidade passiva. Estes processos refletem-se na viabilidade, no bem-estar animal e nos índices de crescimento e ...


Saliva as a potential non-invasive fluid for passive immune transfer surveillan...

Silva, Flávio; Lamy, Elsa; Pedro, Sofia; Azevedo, Inês; Caetano, Pedro; Ramalho, Joana; Martins, Luís; Cerqueira, Joaquim; Silva, Severiano

Passive immune transfer (PIT) is detrimental to the calf’s welfare. PIT is currently assessed by immunoglobulins or total proteins concentration (TP) in blood. Blood collection has some constraints, ergo, a simple and non-invasive alternative method is of great interest. This study aimed to evaluate the potential use of saliva as a non-invasive fluid to assess PIT. A total of eighty saliva and blood samples wer...


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