1830 documents found, page 1 of 183
Cryogenic, but not hypothermic, preservation disrupts the extracellular matrix ...
Ribeiro, Sara Cristina Freitas; Carvalho, A. F.; Rodrigues, Daniel Barreira; Martins, L.; Pires, R. A.; Mendes, V. M.; Manadas, B.; Jarnalo, M.Cell sheet (CS)-based approaches hold significant potential for tissue regeneration, relying on the extracellular matrix (ECM) for success. Like in native tissues, the ECM provides structural and biochemical support for cellular homeostasis and function. Effective preservation strategies that maintain ECM integrity are critical to enhance the therapeutic potential of CS-based approaches. While cryogenic and hyp...
Development and in vitro assessment of injectable, adhesive, and self-healing c...
Correia, Cátia Sofia Palma; Peixoto, D.; Soares da Costa, Diana; Reis, R. L.; Pashkuleva, I.; Alves, N. M.Injured spinal cords have a limited ability to regenerate because of the inhibitory environment formed in situ that affects neuronal regrow. Ensuring stable contact between the injuried nerves to support neural regeneration in the lesion microenvironment remains a significant challenge. To address this challenge, we have engineered a new injectable and adhesive hydrogel to treat spinal cord injuries. This hydro...
The effect of conditioned medium from human amniotic membrane-derived mesenchym...
Silva, C. S.; Casanova, Marta Alexandra Rodrigues; Ferreirinha, P.; da Silva Morais, A.; Osório, H.; Lima, A. C.; Fangueiro, J. F.; Cerqueira, M. T.Aims: The development and selection of T cells occur within the thymus. This organ involutes throughout life, compromising the generation of T cells and, consequently, the efficacy of the immune system. Mesenchymal stem cells (MSC) have beneficial effects on the immune system. Therefore, MSC have been applied in different pathologies related with thymic function. However, there is a lack of knowledge on the pot...
MicroVasculoid-chip: a 3D self-assembled human microcirculation-on-a-chip model...
Abreu, Catarina M.; Lima, Ana Cláudia Fernandes; Neves, N. M.; Kundu, Subhas C; Reis, R. L.; Caballero, DavidThe microvasculature within the tumor microenvironment is crucial for the invasion and dissemination of cancer cells throughout the body. Given its importance and dynamic behavior, several microfluidic models have been developed to study microvascular infiltration and its interaction with cancer cells. However, most of these models primarily focus on blood vessels and use microfluidic channels coated with endot...
Patterned glycopeptide-based supramolecular hydrogel promotes the alignment and...
Castro, Vânia Isabel Baptista; Amorim, Sara; Caballero, David; Abreu, Catarina M.; Kundu, Subhas C; Reis, R. L.; Pashkuleva, I.; Pires, R. A.The functional restoration of a damaged cardiac tissue relies on a synchronized contractile capacity of exogenous and/or endogenous cardiomyocytes, which is challenging to achieve. Here, we explored the potential of the short glycopeptide diphenylalanine glucosamine-6-sulfate (FFGlcN6S) conjugated with an aromatic moiety, namely fluorenylmethoxycarbonyl (Fmoc), to enhance cardiac tissue regeneration. At physiol...
Effect of hyaluronan molecular weight on the stability and biofunctionality of ...
Albuquerque, A. V.; Soares da Costa, D.; Correia, Cátia Sofia Palma; Reis, R. L.; Alves, N. M.; Costa, Rui Filipe Ramos; Pashkuleva, I.Nervous system disorders are characterized by a progressive loss of function and structure of neurons that ultimately leads to a decline in cognitive and motor functions. In this study, we used interfacial polyelectrolyte complexation (IPC) to produce fibers for neural tissue regeneration. IPC is a processing method that allows spinning of sensitive biopolymers. The rate of spinning and the properties of the us...
Enhanced cell uptake of Rhodamine B for photodynamic therapy under hypoxic cond...
Caldas, M.; Artiga, Á.; Marin, R.; Ming, L.; Jaque, D.; da Silva, Lucília Pereira; Reis, R. L.; Correlo, V. M.Photodynamic therapy (PDT) is a promising light-triggered therapy for medical conditions, including solid tumors, and it is already being used in clinical settings. PDT relies on the use of a photosensitizer that generates cytotoxic oxygen species upon photoexcitation. Because of their high molar extinction coefficient, Rhodamine (Rh) dyes are promising candidates as photosensitizers. However, the low solubilit...
Nano-armed Limosilactobacillus reuteri for enhanced photo-immunotherapy and mic...
Xu, Haiting; Wang, Yajun; Liu, Ga; Zhu, Zhenhua; Shahbazi, Mohammad-Ali; Reis, R. L.; Kundu, Subhas C; Shi, Xiaoxiao; Zu, Menghang; Xiao, BoDespite being a groundbreaking approach to treating colorectal cancer (CRC), the efficacy of immunotherapy is significantly compromised by the immunosuppressive tumor microenvironment and dysbiotic intestinal microbiota. Here, leveraging the superior carrying capacity and innate immunity-stimulating property of living bacteria, a nanomedicine-engineered bacterium, LR-S-CD/CpG@LNP, with optical responsiveness, i...
Precise multiphase hydrogel engineering of miniaturized 3D cancer architectures...
Rial, Ramón; Guimarães, Carlos Ferreira; Gasperini, Luca; Brito, Alexandra; Costa, Rui R.; Ruso, Juan M.; Reis, R. L.Understanding cancer biology and therapy responses requires accurate in vitro models that reflect tumor complexity. This work presents a multiphase microfluidic biofabrication approach for creating self-standing three-dimensional (3D) tumor models within hydrogel microfiber boundaries. A single framework enabled the fast generation of different in vitro cellular configurations, including discrete spheroids ...
3D tubular constructs based on natural polysaccharides and recombinant polypept...
Rodrigues, Luísa Cidália Guimarães; Gomes, J. M.; Soares da Costa, Diana; Fernandes, Emanuel Mouta; Costa, R. R.; Rodríguez-Cabello, J. C.; Silva, S. S.The development of versatile tubular structures is critical for tissue engineering (TE) applications where vascularization is necessary. This study investigates the fabrication of tubular shaped biomaterials focused on chitosan (CHT) combined with alginate (ALG) and acemannan (ACE), known for their synergistic properties, including physical stability, antibacterial activity, and healing promotion. Translating t...