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Purple Biodeterioration in Parchment: a Case in the Alcobacense Collection

Pinheiro, C; Miller, A; Bhattacharya, S; Vaz, P; Caldeira, A Teresa; Casanova, C

The understanding of purple biodeterioration, or the appearance of purple tinged stains in parchment, has seen a sharp evolution in the last few years. Modern molecular analysis of the DNA, using Next-Generation Sequencing, have made it possible to hypothesize a chain of events triggered by the presence of Archaeobacteria in the parchment, a succession theory that builds on the presence of complex biological co...


Novas fronteiras na deteção e biocontrolo de comunidades microbianas em patrimó...

Caldeira, A Teresa; Candeias, A

Nos últimos anos, o interesse na compreensão do papel dos microrganismos que colonizam superfícies patrimoniais e nas metodologias para a sua caracterização aumentou significativamente. O desenvolvimento de técnicas avançadas de sequenciação de DNA e análises metagenómicas permitiu uma compreensão mais profunda das comunidades microbianas e dos seus impactos. Além disso, técnicas de microscopia e microanálise a...


Prospection of the red biological patinas influencing the urban scenery archite...

Sitzia, F; Lisci, C; Dias, L; Arantes, S; Caldeira, A Teresa

Portugal’s architecture reflects a rich history influenced by Roman, Moorish, Neoclassical and Romantic styles, with the 20th century marked by Art Deco and colonial influences. Regional styles vary, with white-painted buildings commonly experiencing color changes due to biofilm formation. Visually striking are the red-colored biological patinascommon in the coastal areas. A survey of 120 historical buildings a...


From cultural and natural heritage to reservoir of biomedicine: Prospection of ...

Gatinho, P; Salvador, Cátia; Gutierrez-Patricio, S; Arantes, S; Martins, M R; Silva, A; Miller, A; Caldeira, A Teresa

Caves, with distinctive features and specialized ecosystems, harbour microorganisms that have adapted metabolically to survive in seemingly harsh conditions with limited essential elements. Despite the potential threat of biodeterioration to subterranean heritage due to microbial proliferation, caves serve as vital reservoirs of bioactive compounds produced by subsurface-adapted microorganisms. These microorgan...


The Decorated Garden Grotto of Condes de Basto Palace in Évora, Portugal: Micro...

Silva, I; Salvador, Cátia; Arantes, S; Miller, A; Candeias, A; Caldeira, A Teresa

The Eugénio de Almeida Foundation’s Casa de Fresco is a historical monument of valuable historic–artistic significance, which currently reveals an assortment of biofilms due to the proliferation of microorganisms in the stone and rocaille elements. The biodeterioration in this area was studied as part of the Conservation and Restoration Project. We effectively characterized the local microbial community using m...


Microbial induced stone discoloration in alcobaça monastery: A comprehensive study

Silva, I; Dias, L; Salvador, Cátia; Miller, A; Candeias, A; Caldeira, A Teresa

The Alcobaça Monastery (Portugal), a UNESCO World Heritage Site, currently exhibits a high degree of surface alterations of the stone architectural elements inside the church, including an extensive pink coloration in the walls and columns, bacteria biofilms, and salt efflorescences. The main goal was to identify the microbiota that colonizes the walls and columns of this monument, to help custodians and conser...


Microbial Assessment in A Rare Norwegian Book Collection: A One Health Approach...

Sequeira, S; Pasnak, E; Viegas, C; Gomes, B; Dias, M; Cervantes, R; Pena, P; Twarużek, M; Kosicki, R; Viegas, S; Caetano, L; Penetra, M J; Silva, I

Microbial contamination poses a threat to both the preservation of library and archival collections and the health of staff and users. This study investigated the microbial communities and potential health risks associated with the UNESCO-classified Norwegian Sea Trade Archive (NST Archive) collection exhibiting visible microbial colonization and staff health concerns. Dust samples from book surfaces and the st...


Connecting molecular biomarkers, mineralogical composition, and microbial diver...

Palma, V; González-Pimentel, J; Jimenez-Morillo, N; Sauro, F; Gutiérrez-Patricio, S; De la Rosa, JM; Tomasi, I; Massironi, M; Onac, B; Tiago, I

Lanzarote (Canary Islands, Spain) is one of the best terrestrial analogs to Martian volcanology. Particularly, Lanzarote lava tubes may offer access to recognizably preserved chemical and morphological biosignatures valuable for astrobiology. By combining microbiological, mineralogical, and organic geochemistry tools, an indepth characterization of speleothems and associated microbial communities in lava tubes ...


Design and Experimental Evaluation of a New RNA-FISH Probe to Detect and Identi...

Arantes, S; Branco, P; Caldeira, A Teresa

Paenibacillus, rod-saped gram-positive endospores forming aerobic or facultative anaerobic bacteria, colonize diverse ecosystems and are involved in the biodegradation of cultural heritage assets. Biodeteriogenic microorganisms can be easily detected/identified by ribonucleic acid- fluorescent in situ hybridization RNA-FISH with specific probes. In this work, probes designed in silico were analyzed to calculate...


Methodology for the Synthesis of Chitosan Derivatives to be Applied in Cultural...

Martins, S; Salvador, Cátia; Candfeias, A; Caldeira, A Teresa; Pereira, A

The biological growth and microbial activity in heritage artefacts, promotes and accelerates their deterioration, constituting a reason of great concern for its desirable preservation. The aim of this work is to establish a simple and efficient methodology for the synthesis of new chitosan derivatives, to be applied in the production of new antibacterial biocides. The chitosan derivatives were evaluated for the...


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