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Biosynthesis of Silver-Based Nanoparticles Using Supernatants of Microbial Cult...

Carrapiço, António; Dias, Luís; Carreiro, Elisabete; Caldeira, Ana Teresa; Martins, Maria Rosário

Microbial contamination of Cultural Heritage (CH) materials is one of its most prevalent and impacting preservation issues that can lead to visual, structural, and chemical changes1. Currently, different approaches try to address this issue by employing products and techniques that can lead to structural or chemical alteration of materials2 and pose several environmental risks3. For these reasons, nanotechnolog...


Rational Design of Cost-Effective 4-Styrylcoumarin Fluorescent Derivatives for ...

Eustáquio, Raquel; Ramalho, João Paulo; Caldeira, Ana Teresa; Pereira, António

Fluorescent labels are key tools in a wide range of modern scientific applications, such as fluorescence microscopy, flow cytometry, histochemistry, direct and indirect immunochemistry, and fluorescence in situ hybridization (FISH). Small fluorescent labels have important practical advantages as they allow maximizing the fluorescence signal by binding multiple fluorophores to a single biomolecule. At present, t...


New 4-styrylcoumarin Derivatives as Potentials Fluorescent Labels for Biomolecu...

Eustáquio, Raquel; Caldeira, Ana Teresa; Arantes, Sílvia; Candeias, António; Pereira, António

Fluorescence microscopy is a highly sensitive imaging technique used in various scientific fields such as cellular biology, environmental sciences, medicine, and pharmacy. It offers the advantage of using multiple fluorescent labels to visualize different biomolecules and generate multicolored images for identifying specific components within complex biomolecular structures and studying their interactions. Thes...


Affordable Small Molecules as Promising Fluorescent Labels for Biomolecules

Eustáquio, Raquel; Ramalho, João Paulo; Arantes, Sílvia; Candeias, António; Caldeira, Ana Teresa; Pereira, António

Fluorescent labels, commonly used in highly sensitive analytical techniques for detecting and tracking biomolecules in critical fields like cellular biology, medicine, medicinal chemistry, and environmental science, are currently too expensive for routine use in standard applications, with most exhibiting small Stokes shifts. This limitation underscores the potential of 4-diethylaminobenzaldehyde derivatives as...


Fear of COVID-19 among professional caregivers of the elderly in Central Alente...

Mendes, Felismina; Sim Sim, Margarida; Gemito, Laurência; Barros, Maria da Luz; Serra, Isaura; Caldeira, Ana Teresa

The coronavirus disease 2019 (COVID-19) has infected many institutionalised elderly people. In Portugal, the level of pandemic fear among professional caregivers of the elderly is unknown, as are its predictive factors. This study aimed to investigate predictors of fear of COVID-19 among workers caring for institutionalised elderly people in nursing homes. This is a cross-sectional study using multiple linear r...


Combining in situ elemental and molecular analysis: The Viceroys portraits in O...

Machado, Ana; Valadas, Sara; vandenabeele, Peter; Caldeira, Ana Teresa; Piorro, Luís; Reis, Teresa; Candeias, António

The Vice-Roy's Portraits Gallery hosted at the Old Goa Museum of the Archaeological Survey of India in Goa, India, is a unique panel painting collection, spanning from the 16th till the 18th centuries, and representing the Portuguese Viceroys and Governors who administrated the Portuguese provinces in the coastal region of the Indian Ocean. During the Old Goa Revelations project (a collaborative inter-instituti...


Prospection of the Red Biological Patinas Influencing the Urban Scenery Archite...

Sitzia, Fabio; Lisci, Carla; Dias, Luís; Arantes, Sílvia; Caldeira, Ana Teresa

Portugal’s architecture reflects a rich history influenced by Roman, Moorish, Neoclassical and Romantic styles, with the 20th century marked by Art Deco and colonial influences. Regional styles vary, with white-painted buildings commonly experiencing color changes due to biofilm formation. Visually striking are the red-colored biological patinascommon in the coastal areas. A survey of 120 historical buildings a...


Microbial induced stone discoloration in alcobaça monastery: A comprehensive study

Silva, Inês; Salvador, Cátia; Miller, Ana; Dias, Luís; Candeias, António; Caldeira, Ana Teresa

The Alcobaça Monastery (Portugal), a UNESCO World Heritage Site, currently exhibits a high degree of surface alterations of the stone architectural elements inside the church, including an extensive pink coloration in the walls and columns, bacteria biofilms, and salt efflorescences. The main goal was to identify the microbiota that colonizes the walls and columns of this monument, to help custodians and conser...


Assessment of microbial communities affecting Almada Negreiros’ mural paintings...

Dias, Luís; Gil, Milene; Candeias, António; Caldeira, Ana Teresa

The mural paintings by Almada Negreiros at Lisbon’s Maritime Station of Rocha do Conde de Óbidos, created in 1949, face deterioration due to environmental exposure and past conservation treatments. In 2022, during an on-site analytical campaign aimed at clarifying the artist’ modus operandi and assessing the condition of the paint layers, some areas showed signs of biocolonization and were sampled for analysis....


The Role of Microorganisms in Pink Stone Discoloration: Insights from Batalha M...

Silva, Inês; Salvador, Cátia; Miller, Ana; Candeias, António; Caldeira, Ana Teresa

The colonization of historical buildings and monuments by fungi, algae, and bacteria is a common phenomenon. This often leads to deterioration processes that cause either visual or structural harm. The Batalha Monastery in Portugal, a UNESCOWorld Heritage Site, currently shows significant surface changes to the stone architectural elements within both the Founder’s Chapel and the church, including a widespread ...


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