15 documents found, page 1 of 2
Production and characterization of new fibrinolytic protease from Mucor subtill...
Nascimento, Thiago Pajeú; Sales, Amanda Emmanuelle; Porto, Camila Souza; Brandão, Romero Marcos Pedrosa; Takaki, G. M. Campos; Teixeira, J. A.Fibrinolytic enzymes have received attention regarding their medicinal potential for thrombolytic diseases, a leading cause of morbidity and mortality worldwide. Various natural enzymes purified from animal, plant and microbial sources have been extensively studied. The aim of this work was to produce fibrinolytic protease by solid state fermentation using agro industrial substrates. Rhizopus arrhizus var. arrh...
Screening of wild type Streptomyces isolates able to overproduce clavulanic acid
Marques, Daniela A. Viana; Santos-Ebinuma, Valéria de Carvalho [UNESP]; Oliveira, Patrícia Maria Sobral de; Lima, Gláucia Manoella de SouzaMade available in DSpace on 2015-02-02T12:39:27Z (GMT). No. of bitstreams: 0 Previous issue date: 2014-09-01Bitstream added on 2015-02-02T13:08:32Z : No. of bitstreams: 1 S1517-83822014000300022.pdf: 1163361 bytes, checksum: f6578eb61c12ca4b15b58b2bb5a98706 (MD5); Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP); Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq); Coordenação de A...
Fibrinolytic protease production by new Streptomyces sp. DPUA 1576 from amazon ...
Silva, Germana Michelle de Medeiros e; Bezerra, Raquel P.; Teixeira, J. A.; Porto, Tatiana Souza; Lima-Filho, José. L.; Porto, Ana L. F.Background Streptomyces sp. DPUA 1576 from Amazon lichens was studied to protease and fibrinolytic production. A 22 factorial experimental design was applied to optimize its protease enzyme production using two independent variables, namely soybean flour and glucose concentrations. Results The optimal conditions to obtain high protease production (83.42 U/mL) were 1.26% soybean flour and 1.23% glucose concentra...
Seleção de fungos produtores de β-D-frutosiltransferase por fermentação em esta...
Silva, Juanize M.; Teixeira, J. A.; Porto, Camila S.; Porto, Ana L. F.A enzima β-D-frutosiltransferase é responsável pela síntese de FOS (frutooligossacarídeos) a partir de sacarose por reação de transfrutosilação é produzida por diferentes micro-organismos, principalmente por fungos filamentosos. O objetivo deste trabalho foi selecionar a melhor linhagem fúngica produtora da -D-frutosiltransferase por fermentação em estado sólido, bem como o método de extração. A fermentação em ...
Stability of clavulanic acid in PEG/citrate and liquid–liquid extraction in aqu...
Carneiro-da-Cunha, M. N.; Souza, K. P. S.; Mota, A.; Teixeira, J. A.; Porto, C. S.; Porto, Tatiana Souza; Porto, Ana L. F.β-Lactamases are enzymes responsible for the hydrolysis of β-lactam antibiotics, being produced by several pathogenic bacteria. Clavulanic acid is a commercially and clinically important β-lactamase inhibitor, its extraction being possible by the application of aqueous two-phase system. In this study, clavulanic acid stability was investigated at different molar mass PEG (400, 1 000 and 20 000 g mol−1) and at d...
Purification of polygalacturonases produced by Aspergillus niger using an aqueo...
Maciel, Marília de Holanda Cavalcanti; Ottoni, C. A.; Herculano, Polyanna N.; Porto, Tatiana Souza; Porto, Ana L. F.; Santos, Cledir; Lima, NelsonThe partitioning and purification of polygalacturonases (PG) produced by Aspergillus niger URM 5162 were investigated in aqueous two-phase systems (ATPS), formed by polyethylene glycol and phosphate salts (PEG/phosphate). To evaluate the effect of the 4 independent variables – molar mass of polyethylene glycol (PEG) (400–8000 g/mol – MPEG), PEG concentration (12.5–17.5%, w/w – CPEG), phosphate concentration (15...
Partitioning and purification of polygalacturonases produced by Aspergillus nig...
Maciel, M. H. C.; Ottoni, C. A.; Herculano, P. N.; Porto, T. S.; Porto, Ana L. F.; Santos, C.; Lima, Nelson; Moreira, K. A.; Motta, Cristina SouzaPectinases, or pectinolytic enzymes, are naturally produced by plants, filamentous fungi, bacteria and yeasts. The pectinases are of great importance to clarify and reduce viscosity in fruit juices, improving and increasing tbe filtration efficiency. When used in the crushing of grapes or wine must improve juice extraction, reduce the time to clarify and enhance tbe content ofterpenes in wine. The filamentous f...
Recovery and partitioning of fibrinolytic protease from Bacillus sp. UFPEDA 485...
Souza, F. A. S. D.; Nascimento, J. C. S.; Marques, D. A. V.; Marcos, João Carlos; Porto, Tatiana Souza; Teixeira, J. A.; Porto, Ana L. F.Fibrinolytic proteases produced by Bacillus sp. has attracted interest in the pharmaceutical industry as a promising alternative in thrombolytic therapy due to their effectiveness in degrading fibrin, its production requiring the development of an efficient recovery process. Aqueous two-phase systems (ATPS) have been recognized as an efficient and economical process for recovering enzymes. To optimize the recov...
Effect of pH and temperature on phytase and biomass production by submerged fer...
Nascimento, J. C. S.; Souza, F. A. S. D.; Porto, Tatiana Souza; Mussatto, Solange I.; Teixeira, J. A.; Porto, Ana L. F.Phytase production and biomass was evaluated in present work by submerged fermentation with Aspergillus niger var. phoenicis URM 4924. Experimental assays were done under different conditions of pH (4.0 to 8.0) and temperature (25 to 35 ºC), and the influence of these variables on the responses was studied through a 22 central composite design and response surface methodology. Phytase and biomass production wer...
Integrated process production and extraction of the fibrinolytic protease from ...
Sales, Amanda Emmanuelle; Souza, Fabiana América Silva Dantas de; Teixeira, J. A.; Porto, Tatiana Souza; Porto, Ana L. F.Fibrinolytic proteases are enzymes that degrade fibrin; these enzymes are a promising alternative for thrombolytic therapy, and microorganisms produce them. The aim of this study was to evaluate the optimum conditions for the integrated production and purification of fibrinolytic protease from Bacillus sp. UFPEDA 485. Extractive fermentation was carried out in a culture medium containing soybean flour and by ad...