30 documents found, page 1 of 3
Cattle as reservoirs of clinically relevant Enterococcus: A One Health genomic ...
Ribeiro, Inês M.; Almeida-Santos, Ana C.; Teixeira, Maria J.; Ribeiro, A.; Campos, Carla; Brito, Nuno V.; Dantas, Rui; Pinho, Luís; Quinteira, SandraBackground: Enterococcus spp. are opportunistic pathogens and One Health indicators of antimicrobial resistance (AMR) [1]. Cattle remain understudied reservoirs of multidrug-resistant (MDR) enterococci [2]. This study assessed the occurrence of clinically relevant antibiotic-resistant Enterococcus in cattle farms in Northern Portugal, comparing intensive dairy systems and native breeds under extensive condition...
Multidrug-resistant Enterococcus spp. in cattle farm environments: a One Health...
Madureira, Catarina; Ribeiro, Inês M.; Neto, Beatriz; Pinho, Luís; Campos, Carla; Quinteira, Sandra; Peixe, Luísa; Almeida-Santos, Ana C.; Novais, CarlaBackground: Enterococcus spp. are commensals of humans and animals but also important opportunistic pathogens and reservoirs of antimicrobial resistance (AMR) [1]. Data on antibiotic-resistant enterococci in cattle remain limited in Europe, including Portugal [2,3], and the contribution of farm environments to AMR dissemination is largely unknown. Objective: To assess the occurrence and AMR profiles of Ent...
Microbial Contamination in Saturated Saline Solution Used for Cadaver Preservat...
Almeida, Marisa; Dias, Francisca; Pissarra, Helga; Sá, Sónia; Lei, Mariana; Campos, Carla; Freitas, Ana R.; Quinteira, Sandra; Alvites, RuiBackground: The use of animal cadavers is essential for veterinary anatomy education, requiring preservation methods that maintain tissue characteristics similar to those of living organisms. At CESPU, cadavers are preserved using a saturated saline (SS) solution, considered effective in maintaining tissue texture, color, and joint mobility [1,2]. However, its ability to control microbial growth, particularly f...
Identification of linezolid-resistant Enterococcus spp. in calves: findings fro...
Ribeiro, Alícia; Teixeira, Maria J.; Pinho, Luís; Campos, Carla; Novais, Carla; Peixe, Luísa; Brito, Nuno V.; Quinteira, Sandra; Miranda, CarlaBackground: Antimicrobial resistance (AMR) is a global health threat requiring a One Health approach, as antibiotic-resistant bacteria can spread between animals and humans. Enterococcus spp., particularly E. faecium (Efm) and E. faecalis (Efs), are key AMR indicators due to their role as gut commensals and their potential as reservoirs of resistance genes. They are also opportunistic pathogens that can cause s...
Diagnostic pitfalls in Staphylococcus spp. surveillance: lessons from a multina...
Pinheiro, Bárbara; Gomes-Sampaio, Luís M.; Pacheco, Patrícia; Campos, Carla; Braz, Maria Pranto; Coelho, Cristina; Miranda, Carla; Quinteira, SandraBackground: Staphylococcus aureus is a leading cause of severe and hard-to-treat human infections, particularly when resistant to cefoxitin due to the presence of the mecA gene [1]. However, diagnostic challenges arise from the misidentification of S. aureus and related species when relying on classical identification methods (mannitol fermentation; coagulase production), as well as from the detection of strain...
Diagnostic pitfalls in Staphylococcus spp. surveillance: lessons from a multina...
Pinheiro, Bárbara; Gomes-Sampaio, Luís M.; Pacheco, Patrícia; Campos, Carla; Braz, Maria Pranto; Coelho, Cristina; Miranda, Carla; Quinteira, SandraStaphylococcus aureus is a leading cause of severe and hard-to-treat human infections, particularly when resistant to cefoxitin due to the presence of the mecA gene [1]. However, diagnostic challenges arise from the misidentification of S. aureus and related species when relying on classical identification methods (mannitol fermentation;coagulase production), as well as from the detection of strains carrying th...
Identification of linezolid-resistant Enterococcus spp. in calves: findings fro...
Ribeiro, Alícia; Teixeira, Maria J.; Pinho, Luís; Campos, Carla; Novais, Carla; Peixe, Luísa; Brito, Nuno V.; Quinteira, Sandra; Miranda, CarlaAntimicrobial resistance (AMR) is a global health threat requiring a One Health approach, asantibiotic-resistant bacteria canspread between animals and humans. Enterococcusspp., particularly E. faecium(Efm) and E. faecalis(Efs),are keyAMR indicators due to their role as gut commensals and their potential as reservoirs of resistance genes.They are also opportunistic pathogensthat can cause severe human infection...
Colistin-resistant Escherichia coli in calves and adult cattle from Portuguese ...
Ribeiro, Madalena F.; Pinho, Luís; V. Brito, Nuno; Campos, Carla; Freitas, Ana R.; Quinteira, Sandra; Miranda, CarlaBackground: Antimicrobial resistance poses a pressing and significant challenge, driven by the intricate interplay among animals, humans, and the environment [1]. Escherichia coli, a commensal within animal and human gut microbiota, stands as one of the most ubiquitous bacteria. With a multitude of resistance genes, many E. coli strains exhibit a multidrug-resistant phenotype, diminishing the effectiveness...
Mapping of key bacterial species for postmortem interval calculation
Cláudia-Ferreira, Ana; Barbosa, Daniel J.; Campos, Carla; Dinis-Oliveira, Ricardo Jorge; Freitas, Ana R.Background: Estimation of the Postmortem Interval (PMI), the time elapsed since death, is one of the most challenging issues in forensic sciences [1]. Most studies focus on extensive bacterial sequencing, but culture-based experiments for higher taxonomic resolution remain scarce [2]. Objective: We aimed to analyze total bacterial counts and map Enterococcus faecalis, Staphylococcus aureusand Escherichia coli i...
Dairy cattle and the iconic autochthonous cattle in northern Portugal are reser...
Quinteira, Sandra; Dantas, Rui; Pinho, Luís; Campos, Carla; Freitas, Ana R.; Brito, Nuno V.; Miranda, Carla; Campos, CarlaAnimals destined for human consumption play a key role in potentially transmitting bacteria carrying antibiotic resistance genes. However, there is limited knowledge about the carriage of antibiotic-resistant bacteria in native breeds. We aimed to characterize the phenotypic profiles and antibiotic resistance genes in Escherichia coli isolated from bovines, including three native Portuguese bovine breeds. Forty...