6 documents found, page 1 of 1
Proteome dynamics of the cotyledonary haustorium and endosperm in the course of...
Nascimento, José Roberto da Silva; Mélo Neto, Domingos Ferreira; Coutinho, Ítalo C.; Domont, Gilberto B.; Nogueira, Fábio C. S.; Campos, Francisco A. P.The role of the cotyledonary haustorium (CH) in the mobilization of nutrient reserves in the endosperm of species of the palm family Arecaceae is a moot question. To shed light on this matter, we present here an analysis of the quantitative proteome changes associated with four developmental stages of CH and three of endosperm during germination. Together, a total of 1965 proteins were identified, being 1538 in...
Seed development of Jatropha curcas L. (Euphorbiaceae): integrating anatomical,...
Soares, Emanoella L.; Lima, Magda L. B.; Nascimento, José Roberto da Silva; Soares, Arlete A.; Coutinho, Ítalo A. C.; Campos, Francisco A. P.Key message This work provides a detailed histological analysis of the development of Jatropha curcas seeds, together with an assessment of the role of programmed cell death in this process. Abstract Seeds of Jatropha curcas are a potential source of raw material for the production of biodiesel, but very little is known about how the architecture of the seeds is shaped by the coordinated development of the embr...
Deep proteome analysis of gerontoplasts from the inner integument of developing...
Shah, Mohibullah; Soares, Emanoella L.; Lima, Magda L. B.; Pinheiro, Camila B.; Soares, Arlete A.; Domont, Gilberto B.; Nogueira, Fabio C. S.The inner integument of Jatropha curcas seeds is a non-photosynthetic tissue that acts primarily as a conduit for the delivery of nutrients to the embryo and endosperm. In this study we performed a histological and transmission electron microscopy analysis of the inner integument in stages prior to fertilization to 25 days after pollination, to establish the structural changes associated with the plastid to ger...
Differential expression of cysteine peptidase genes in the inner integument and...
Rocha, Antônio J.; Soares, Emanoella L.; Costa, José H.; Costa, Washington L. G.; Soares, Arlete A.; Nogueira, Fábio C. S.; Domont, Gilberto B.In several plant tissues, programmed cell death (PCD) is mediated by the combined action of cysteine peptidases, namely KDEL-tailed cysteine peptidases (KDEL-CysEP) and vacuolar processing enzymes (VPE). Here, we performed a search of the draft genome of Jatropha curcas L. (Euphorbiaceae) and identified 2 genes for KDEL-CysEP (Jc-CysEP1 and Jc-CysEP2) and 3 genes for VPE (Jc-βVPE, Jc-γVPE and Jc-ıVPE) and deter...
Proteomic profile of the nucellus of castor bean (Ricinus communis L.) seeds du...
Nogueira, Fábio C. S.; Palmisano, Giuseppe; Soares, Emanoella L.; Shah, Mohibullah; Soares, Arlete A.; Roepstorff, Peter; Campos, Francisco A. P.In this study, we performed a proteomic analysis of nucellus from two developmental stages of Ricinus communis seeds by a GeLC-MS/MS approach, using of a high resolution orbi trap mass spectrometer, which resulted in the identification of a total of 766 proteins that were grouped into 553 protein groups. The distribution of the identified proteins in stages III and IV into different Gene Ontology categories was...
Proteome analysis of secondary somatic embryogenesis in cassava (Manihot escule...
Ibrahim, Abdulrazak Baba; Nogueira, Fabio C. S.; Pinheiro, Camila B.; Brasil, Juliana N.; Jereissati, Emmanuel S.; Jucá, Thiago LustosaUsing histological analysis of the induction of secondary somatic embryogenesis (SSE) in cassava (Manihot esculenta Crantz) as a guide, we performed 2-DE for protein separation and matrix-assisted laser desorption ionization-time of flight-tandem mass spectrometry (MALDI-TOF-TOF-MS) for protein identification in cotyledons of cassava somatic embryos undergoing SSE. Reference map obtained by 2- DE within a pH ra...