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Earthworms and their cutaneous excreta can modify the virulence and reproductiv...

Chelkha, Maryam; Blanco-Pérez, Rubén; Vicente-Díez, Ignacio; Bueno-Pallero, Francisco; Amghar, Souad; El Harti, Abdellatif; Campos-Herrera, Raquel

Earthworms are ecological engineers that can contribute to the displacement of biological control agents such as the entomopathogenic nematodes (EPNs) and fungi (EPF). However, a previous study showed that the presence of cutaneous excreta (CEx) and feeding behavior of the earthworm species Eisenia fetida (Haplotaxida: Lumbricidae) compromise the biocontrol efficacy of certain EPN species by reducing, for examp...


Cutaneous excreta of the earthworm Eisenia fetida (Haplotaxida: Lumbricidae) mi...

Chelkha, Maryam; Blanco-Perez, Ruben; Bueno-Pallero, Francisco Angel; Amghar, Souad; El Harti, Abdellatif; Campos-Herrera, Raquel

Earthworms enhance soil structure, the decomposition of organic matter and the dissemination of beneficial soil organisms such as the entomopathogenic nematodes (EPNs). Nevertheless, the effects of earthworm feeding behavior or cutaneous excreta (CEx) on the performance of EPNs as biological control agents is poorly understood. We hypothesized that the presence of earthworms or their excreta reduces EPN fitness...


Vegetation drives assemblages of entomopathogenic nematodes and other soil orga...

Campos-Herrera, Raquel; Blanco-Pérez, Rubén; Bueno-Pallero, Francisco Ángel; Duarte, Amílcar; Nolasco, Gustavo; Sommer, Ralf J.

Entomopathogenic nematodes (EPNs) are widely distributed in soils throughout the world. Their activity as biological control agents is modulated by abiotic and biotic factors (e.g. soil type, climatic fluctuation and natural enemies). We sought to identify soil properties in a Mediterranean region, which might be managed to enhance biological control agents’ services provided by EPNs. We hypothesized that respon...


Scavenging behavior and interspecific competition decrease offspring fitness of...

PEREZ, RUBEN; Bueno-Pallero, Francisco Angel; Vicente-Diez, Ignacio; Santiago Marco-Mancebon, Vicente; Perez-Moreno, Ignacio; Campos-Herrera, Raquel

Entomopathogenic nematodes (EPNs) are well-studied biocontrol agents of soilw-dwelling arthropod pests. The insecticidal efficiency of EPNs is modulated by food web dynamics. EPNs can reproduce in freeze-killed insect larvae, even in competition with free-living bacterivorous nematodes (FLBNs) in the genus Oscheius. The objective of this study was to assess the efficiency of EPNs as scavengers when competing wi...


Steinernema khuongi n. sp. (Panagrolaimomorpha, Steinernematidae), a new entomo...

Stock, S. P.; Campos-Herrera, Raquel; El-Borai, F. E.; Duncan, L. W.

In this study, molecular (ribosomal sequence data), morphological and cross-hybridization properties were used to identify a new Steinernema sp. from Florida, USA. Molecular and morphological data provided evidence for placing the novel species into Clade V, or the 'glaseri-group' of Steinernema spp. Within this clade, analysis of sequence data of the rDNA genes, 28S and internal transcribed spacer (ITS), depic...


Combined field inoculations of pseudomonas bacteria, arbuscular mycorrhizal fun...

Imperiali, Nicola; Chiriboga, Xavier; Schlaeppi, Klaus; Fesselet, Marie; Villacres, Daniela; Jaffuel, Geoffrey; Bender, S. Franz; Dennert, Francesca

In agricultural ecosystems, pest insects, pathogens, and reduced soil fertility pose major challenges to crop productivity and are responsible for significant yield losses worldwide. Management of belowground pests and diseases remains particularly challenging due to the complex nature of the soil and the limited reach of conventional agrochemicals. Boosting the presence of beneficial rhizosphere organisms is a...


Spatial relationships between entomopathogenic nematodes and nematophagous fung...

Pathak, Ekta; Campos-Herrera, Raquel; El-Borai, Fahiem E.; Duncan, Larry W.

Relationships between entomopathogenic nematodes (EPNs), nematophagous fungi (NF) and soil physical and chemical properties were studied in a survey of 53 citrus orchards in central ridge and flatwoods ecoregions of Florida. Seven species of NF associated with nematodes were quantified directly using a real time qPCR assay. All nematophagous fungi studied except Arthrobotrys musiformis and Hirsutella rhossilien...


Reproductive efficiency of entomopathogenic nematodes as scavengers. Are they a...

Blanco-Pérez, Rubén; Bueno-Pallero, Francisco Ángel; Neto, Luis; Campos-Herrera, Raquel

Entomopathogenic nematodes (EPNs) and their bacterial partners are well-studied insect pathogens, and their persistence in soils is one of the key parameters for successful use as biological control agents in agroecosystems. Free-living bacteriophagous nematodes (FLBNs) in the genus Oscheius, often found in soils, can interfere in EPN reproduction when exposed to live insect larvae. Both groups of nematodes can...


Effects of cover crops on the overwintering success of entomopathogenic nematod...

Jaffuel, Geoffrey; Blanco-Pérez, Rubén; Büchi, Lucie; Mäder, Paul; Fließbach, Andreas; Charles, Raphaël; Degen, Thomas; Turlings, Ted C.J.

Conservation agriculture is arising as an alternative to conventional agriculture with the aim to have a reduced impact on the environment. This includes the use of cover crops to conserve soil quality by limiting soil erosion, adding organic matter, and enhancing soil nutrient content, as well as water availability, which are all factors that can greatly influence the presence of soil organisms. In the current...


Effect of soil properties, heavy metals and emerging contaminants in the soil n...

Gutierrez, Carmen; Fernandez, Carlos; Escuer, Miguel; Campos-Herrera, Raquel; Beltran Rodriguez, Ma Eulalia; Carbonell, Gregoria

Among soil organisms, nematodes are seen as the most promising candidates for bioindications of soil health. We hypothesized that the soil nematode community structure would differ in three land use areas (agricultural, forest and industrial soils), be modulated by soil parameters (N, P, K, pH, SOM, CaCO3, granulometric fraction, etc.), and strongly affected by high levels of heavy metals (Cd, Pb, Zn, Cr, Ni, C...


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