17 documents found, page 1 of 2
Bioengineered nanoparticles loaded-hydrogels to target TNF Alpha in inflammator...
Oliveira, Isabel Matos; Fernandes, Diogo Castro; Maia, F. Raquel; Canadas, Raphael Faustino; Reis, R. L.; Oliveira, J. M.Rheumatoid Arthritis (RA) is an incurable autoimmune disease that promotes the chronic impairment of patientsâ mobility. For this reason, it is vital to develop therapies that target early inflammatory symptoms and act before permanent articular damage. The present study offers two novel therapies based in advanced drug delivery systems for RA treatment: encapsulated chondroitin sulfate modified poly(amidoamine...
3DICE coding matrix multidirectional macro-architecture modulates cell organiza...
Canadas, Raphael Faustino; Costa, João Pedro Bebiano; Mao, Zhengwei; Gao, Changyou; Demirci, Utkan; Reis, R. L.; Marques, A. P.; Oliveira, J. M.Natural extracellular matrix governs cells providing biomechanical and biofunctional outstanding properties, despite being porous and mostly made of soft materials. Among organs, specific tissues present specialized macro-architectures. For instance, hepatic lobules present radial organization, while vascular sinusoids are branched from vertical veins, providing specific biofunctional features. Therefore, it is...
Entrapped in cage (EiC) scaffolds of 3D-printed polycaprolactone and porous sil...
Cengiz, I. F.; Maia, F. Raquel; da Silva Morais, Alain; Silva-Correia, Joana; Pereira, H.; Canadas, Raphael Faustino; Espregueira-Mendes, JoãoThe meniscus has critical functions in the knee joint kinematics and homeostasis. Injuries of the meniscus are frequent, and the lack of a functional meniscus between the femur and tibial plateau can cause articular cartilage degeneration leading to osteoarthritis development and progression. Regeneration of meniscus tissue has outstanding challenges to be addressed. In the current study, novel Entrapped in Cag...
Convection patterns gradients of non-living and living micro-entities in hydrogels
Canadas, Raphael Faustino; Patrício, Pedro; Brancato, Virginia; Gasperini, Luca; Caballero, David; Pires, Ricardo A; Costa, João B.; Pereira, HélderInducing thermal gradients in two injected fluid systems results in the temporal formation of mixing conductive streams. If preserved through sol-gel transition, this mechanism can be used to drive and pattern non-living and living entities in mixed hydrogels. Interfaces are vital in nature, where gradients of non-living and living entities build distinct yet continuous integrated living tissues. However, the c...
Biochemical gradients to generate 3D heterotypic-like tissues with isotropic an...
Canadas, Raphael Faustino; Ren, Tachen; Marques, A. P.; Oliveira, J. M.; Reis, R. L.; Demirci, UtkanAnisotropic 3D tissue interfaces with functional gradients found in nature are replicated in vitro for drug development and tissue engineering. Even though different fabrication techniques, based on material science engineering and microfluidics, are used to generate such microenvironments, mimicking the native tissue gradient is still a challenge. Here, the fabrication of 3D structures are described with linea...
Bioreactors and microfluidics for osteochondral interface maturation
Canadas, Raphael Faustino; Marques, A. P.; Reis, R. L.; Oliveira, J. M.The cell culture techniques are in the base of any biology-based science. The standard techniques are commonly static platforms as Petri dishes, tissue culture well plates, T-flasks, or well plates designed for spheroids formation. These systems faced a paradigm change from 2D to 3D over the current decade driven by the tissue engineering (TE) field. However, 3D static culture approaches usually suffer from sev...
Posterior talar process as a suitable cell source for treatment of cartilage an...
Correia, Sandra I.; Silva-Correia, Joana; Pereira, H.; Canadas, Raphael Faustino; Morais, Alain; Frias, A. M.; Sousa, R. A.; van Dijk, C.N.Osteochondral defects of the ankle are common lesions affecting the talar cartilage and subchondral bone. Current treatments include cell-based therapies but are frequently associated with donor-site morbidity. Our objective is to characterize the posterior process of the talus (SP) and the os trigonum (OT) tissues and investigate its potential as a new source of viable cells for application in tissue engineeri...
Osteochondral tissue engineering and regenerative strategies
Canadas, Raphael Faustino; Marques, A. P.; Reis, R. L.; Oliveira, J. M.The orthopedic field has been facing challenging difficulties when it comes to regeneration of large and/or complex defects as we come across in osteochondral (OC) cases of lesions grade 4. Autologous OC mosaicplasty has proven to be a valid therapeutic option but donor site morbidity and the lack of long-term functionality remain sources of concern. OC tissue engineering has shown an increasing development to ...
Cartilage and bone regeneration: how close are we to bedside?
Canadas, Raphael Faustino; Pina, S.; Marques, A. P.; Oliveira, Joaquim M.; Reis, R. L.The treatment/regeneration of bone and cartilage diseases or defects, whether induced by rheumatism, joint dysplasia, trauma, or surgery presents great challenges that have not been fully solved by the current therapies. In the last few years, tissue engineering and regenerative medicine have been proposing advanced tools and technologies for bone and cartilage tissue regeneration, and some of which have succes...
Dynamic platform to recreate an osteoarthritic 3D in vitro model
Canadas, Raphael Faustino; Marques, A. P.; Oliveira, J. M.; Reis, R. L.; Demirci, U.Osteoarthritis (OA), a prevalent chronic condition with a striking impact on life quality, represents an enormous societal burden that increases greatly as populations’ age. Yet no approved pharmacological intervention, biologic therapy or procedure prevents the progressive destruction of the OA joint. Based on bilayered structures that have been previously suggested for osteochondral (OC) applications (Oliveir...