66 documents found, page 1 of 7
Behavioural responses to visual and chemical cues in Mozambique tilapia (Oreoch...
Ashoori, Samyar; Canario, Adelino; Hubbard, PeterIn reproduction of Mozambique tilapia (Oreochromis mossambicus), chemical communication mainly relies on urinary steroidal glucuronides (sex pheromones) that dominant males release to prime the final ovulation in pre-ovulatory females and increase reproductive readiness. However, the role of steroids in chemical communication in females has received less attention. Here, we aimed to determine whether 17 beta-es...
Off the shelf: regulation of fish blood pH through ionocyte membrane remodellin...
Guerreiro, Pedro Miguel; Canario, AdelinoOcean acidification (OA), together with warming and deoxygenation, forms the “deadly trio,” a combination that reduces ecosystems’ resilience, makes marine biodiversity vulnerable, and is considered a main contributor to global mass extinctions in the Earth’s history (1).
Circannual prevalence of tetrodotoxins in trumpet shells: sea stars as a possib...
Pais, Maria; Carvalho, Flávia; Frade, Magda; Reis Costa, Pedro; Silva, José Paulo da; Marçalo, Ana; Canario, Adelino; Lage, SandraTetrodotoxin (TTX) is a potent neurotoxin, first identified in fish from the Tetraodontidae family but also detected in marine invertebrates. A Human poisoning episode after consumption of trumpet shell Charonia lampas, likely caught off the Portuguese mainland southern coast - Algarve, together with the increasing reports of TTX in European waters, led the European Food Safety Authority (EFSA) to recommend max...
Bacterial 16S ribosomal gene fingerprints as a tool to diagnose and mitigate fi...
NAJAFPOUR, BABAK; Canario, Adelino; Power, Deborah MaryDysbiosis is associated with shifts in the diversity or relative abundance of beneficial versus harmful bacteria, leading to health issues in organisms. This study investigated gut bacterial dysbiosis associated with larval quality using 16S rRNA gene sequencing. The gut microbiome of gilthead sea bream and European sea bass, key commercial species and vertebrate models, was examined in high- and low-quality la...
A long photoperiod following a short one, and low temperature, stimulate gameto...
Marquet, Nathalie; Canario, AdelinoTemperature and photoperiod are critical regulators of reproduction. However, few studies have evaluated experimentally their specific effects on gametogenesis in sea cucumbers. We tested the effect on gametogenesis of combinations of photoperiod and temperature: "accelerated" (long days following short days; a 12 degrees C deep in temperature), "constant" (constant short photoperiod; constant Spring/Summer tem...
Studies on cortisol, corticosterone, and 17b-estradiol indicate these steroids ...
Maskrey, Benjamin H.; Costas, Carolina; Méndez-Martínez, Luís; Guerrero-Peña, Laura; Tur, Ricardo; García, Pablo; Touriñan, Pablo; Chavarrias, DavidThe common octopus (Octopus vulgaris) is a promising candidate for aquaculture diversification, particularly in Europe. As interest in octopus farming grows, animal welfare concerns arise. In bony vertebrates (teleosts and tetrapods), measurements of the levels of corticosterone or cortisol have been successfully used as indicators of stress and welfare. Here, it is explored whether octopuses also produce corti...
Protocol for isolating nuclei from frozen fish heart tissue for single-cell gen...
Wei, Chaoguang; Jia, Ruonan; Canario, Adelino; Chen, Liangbiao; Xu, Qianghua; Hu, PengUnbiased isolation of intact single nuclei from complex tissues, such as cryopreserved hearts, for massively parallel single-cell genomic assays is particularly challenging. Here, we present a protocol for isolating high-quality nuclei from the heart tissue of Trematomus bernacchii, an Antarctic fish species with notoriously challenging tissue for nuclear extraction. We describe steps for pulverizing frozen hea...
Inversions dominate evolution in the european sardine (sardina pilchardus) amid...
Sabatino, Stephen J.; Cabezas, M. Pilar; Pereira, Paulo; Garrido, Susana; Santos, António M.; Carneiro, Miguel; Santos, Paulo T.; Louro, BrunoInversions can play key roles in the genetic architecture of adaptation, but the scale of their effects across different species remains poorly understood. Here, we use whole-genome sequencing and demographic modelling to investigate the influence of inversions on the population genomics of the r-selected European sardine (Sardina pilchardus). Allele frequency differences from millions of SNPs across 34 populat...
Differential tissue immune stimulation through immersion in bacterial and viral...
Sousa, Carmen; Peng, Maoxiao; Guerreiro, Pedro; Cardoso, João; Chen, Liangbiao; Canario, Adelino; Power, DeborahThe genome evolution of Antarctic notothenioids has been modulated by their extreme environment over millennia and more recently by human -caused constraints such as overfishing and climate change. Here we investigated the characteristics of the immune system in Notothenia rossii and how it responds to 8 h immersion in viral (Poly I:C, polyinosinic: polycytidylic acid) and bacterial (LPS, lipopolysaccharide) pr...
The physiological effect of polystyrene nanoplastic particles on fish and human...
Peng, Maoxiao; Félix, Rute C.; Canario, Adelino; Power, DeborahNumerous studies have identified the detrimental effects for the biosphere of large plastic debris, the effect of microplastics (MPs) and nanoplastics (NPs) is less clear. The skin is the first point of contact with NPs, and skin fibroblasts have a vital role in maintaining skin structure and function. Here, a comparative approach is taken using three fibroblast cell lines from the zebrafish (SJD.1), human male...