176 documents found, page 1 of 18
Mutational Analysis of Colistin-Resistant Pseudomonas aeruginosa Isolates: From...
De Sousa, Telma; Wang, Hsin-Yao; Lin, Ting-Wei; Caniça, Manuela; Ramos, Miguel J.N.; Santos, Daniela; Silva, Catarina; Saraiva, Sónia; Beyrouthy, RachaThis study analyzed eleven isolates of colistin-resistant Pseudomonas aeruginosa, originating from Portugal and Taiwan, which are associated with various pathologies. The results revealed significant genetic diversity among the isolates, with each exhibiting a distinct genetic profile. A prevalence of sequence type ST235 was observed, characterizing it as a high-risk clone, and serotyping indicated a predominan...
Mapping the evidence of the effects of environmental factors on the prevalence ...
Deza-Cruz, Iñaki; de Menezes, Alexandre; Gardner, Brian; Aktan, Ílknur; Alnajjar, Sarhad; Betson, Martha; Cabal Rosel, Adriana; Caniça, ManuelaBackground: Antibiotic resistance increasingly threatens the interconnected health of humans, animals, and the environment. While misuse of antibiotics is a known driver, environmental factors also play a critical role. A balanced One Health approach-including the environmental sector-is necessary to understand the emergence and spread of resistance. Methods: We systematically searched English-language literatu...
Identifying phage Lysins through genomic analysis of prophages from Acinetobact...
Raposo, Maria Leonor; Pimentel, Ana Carolina; Manageiro, Vera; Duarte, Aida; Caniça, Manuela; Vale, Filipa F.Acinetobacter baumannii is a Gram-negative opportunistic pathogen, responsible for nosocomial infections worldwide. In recent years, this microorganism has acquired resistance to various antibiotics, prompting the World Health Organization (WHO) to declare carbapenem-resistant A. baumannii (CRAB) a critical priority microorganism requiring urgent attention and the development of new therapeutic options. Here, w...
Prediction of Antibiotic Resistance Genes in Cyanobacterial Strains by Whole Ge...
Balata, Duarte; Rosado, Tânia; Pina-Martins, Francisco; Manageiro, Vera; Menezes, Carina; Ferreira, Eugénia; Paulo, Octávio S.; Caniça, ManuelaCyanobacteria are ubiquitous in freshwater environments, but their role in aquatic resistome remains unclear. In this work, we performed whole genome sequencing on 43 cyanobacterial strains isolated from Portuguese fresh/wastewaters. From 43 available non-axenic unicyanoabacterial cultures (containing only one cyanobacterial strain and their co-occurring bacteria), it was possible to recover 41 cyanobacterial g...
Unraveling the genome-wide repertoire of the novel chromosomally encoded mcr-8....
Rivière, Rani; Teixeira, Pedro; Silva, Catarina; Ramos, Miguel; Dias, Elsa; Manageiro, Vera; Caniça, ManuelaThe increasing rates of colistin resistance worldwide poses a significant threat to public health. While the most commonly described variant is , other variants such as have been detected, typically associated with . However, little is known about the prevalence of in other bacterial species and environmental reservoirs. This study aimed to characterize a novel subvariant identified in a strain isolated from ma...
Genomic epidemiology and resistome dynamics of species in a Portuguese Open Air...
Teixeira, Pedro; Ramos, Miguel; Rivière, Rani; Azevedo, Mónica; Ferreira, Mário; Cano, Maria Manuela; Vieira, Patrícia; Reis, Lígia; Matias, RuiInterconnected reservoirs contribute to the global spread of antimicrobial resistance (AMR), including carbapenem- and colistin-resistant , highlighting the need for a One Health approach. We assessed the genomic epidemiology, diversity and AMR mechanisms of spp. across interconnected human, animal, plant, and environmental reservoirs in a Portuguese Open Air Laboratory. Over a one year monitoring period, sampl...
Pseudomonas aeruginosa: One Health approach to deciphering hidden relationships...
de Sousa, Telma; Machado, Sandro; Caniça, Manuela; Ramos, Miguel J.N.; Santos, Daniela; Ribeiro, Miguel; Hébraud, Michel; Igrejas, GilbertoAims: Antimicrobial resistance in Pseudomonas aeruginosa represents a major global challenge in public and veterinary health, particularly from a One Health perspective. This study aimed to investigate antimicrobial resistance, the presence of virulence genes, and the genetic diversity of P. aeruginosa isolates from diverse sources. Methods and results: The study utilized antimicrobial susceptibility testing, g...
Genomic Characterization of Multidrug-Resistant and ESBL-Producing Klebsiella p...
Silva, Vanessa; Caniça, Manuela; Rivière, Rani; Silva, Adriana; Poeta, Patrícia; Igrejas, GilbertoKlebsiella pneumoniae is an important pathogen associated with multidrug resistance and virulence in both human and animal populations. While its prevalence and resistance patterns are well documented in clinical settings, data on K. pneumoniae in food-producing animals remain scarce. This study aimed to isolate and characterize multidrug-resistant K. pneumoniae strains from healthy rabbits raised for human con...
Assessment of Antibiotic Resistance Among Isolates of Klebsiella spp. and Raoul...
Sabença, Carolina; de la Rivière, Rani; Barros, Paulo; Cabral, João Alexandre; Sargo, Roberto; Sousa, Luís; Dapkevicius, Maria de Lurdes EnesOne of the significant challenges facing modern medicine is the rising rate of antibiotic resistance, which impacts public health, animal health, and environmental preservation. Evaluating antibiotic resistance in wildlife and their environments is crucial, as it offers essential insights into the dynamics of resistance patterns and promotes strategies for monitoring, prevention, and intervention. and genera is...
Bats as Hosts of Antimicrobial-Resistant Mammaliicoccus lentus and Staphylococc...
Silva, Vanessa; Caniça, Manuela; de la Rivière, Rani; Barros, Paulo; Cabral, João Alexandre; Poeta, Patrícia; Igrejas, GilbertoBats are increasingly recognized as reservoirs for antimicrobial-resistant bacteria, playing a potential role in the dissemination of resistance genes across species and regions. In this study, 105 bats from 19 species in Portugal were sampled to investigate the presence, antimicrobial resistance, and genetic characteristics of Mammaliicoccus and Staphylococcus isolates. Thirteen Mammaliicoccus lentus and Staph...