2 documents found, page 1 of 1
Role of iron in the nitric oxide-mediated fungicidal mechanism of IFN-gamma-act...
Gonzalez, Angel; Restrepo, Angela; Cano, Luz E.Iron is an essential growth element of virtually all microorganisms and its restriction is one of the mechanisms used by macrophages to control microbial multiplication. Paracoccidioides brasiliensis, the agent of paracoccidioidomycosis, an important systemic mycosis in Latin America, is inhibited in its conidia-to-yeast conversion in the absence of iron. We studied the participation of iron in the nitric oxide...
Purification of somatic antigens from Paracoccidioides brasiliensis. Preliminar...
Burgos, Luis C.; Cano, Luz E.; Restrepo, AngelaWe describe the procedures followed to purify antigenic fractions from P. brasiliensis sonicated whole yeast cells. By means of the agar gel immunodiffusion test, antigen-antibody complexes were allowed to produce precipitin lines; these were further separated, eluated and two antibody-free antigenic fractions were further purified by column chromatography and polyacrilamyde gel electrophoreses. These fractions...