12 documents found, page 1 of 2
Multidrug-Resistant Methicillin-Resistant Coagulase-Negative Staphylococci in H...
Silva, Vanessa; Caniça, Manuela; Ferreira, Eugénia; Vieira-Pinto, Madalena; Saraiva, Cândido; Pereira, José Eduardo; Capelo, José LuisCoagulase-negative staphylococci are commensals that are known to be prevalent in most environments, and they are also an important reservoir of antimicrobial-resistant genes. Staphylococcal infections in animal husbandry are a high economic burden. Thus, we aimed to determine the prevalence and species diversity of methicillin-resistant coagulase-negative staphylococci (MRCoNS) in poultry slaughtered for human...
Nocturnal Birds of Prey as Carriers of Staphylococcus aureus and Other Staphylo...
Silva, Vanessa; Lopes, Ana Filipa; Soeiro, Vanessa; Caniça, Manuela; Manageiro, Vera; Pereira, José Eduardo; Maltez, Luís; Capelo, José LuisOwls are nocturnal predators that inhabit urbanized and farmlands. They are in direct contact with other animals, both livestock and small wild rodents that they mostly feed on. Staphylococci can be both commensal and pathogenic bacteria that are widespread across the various ecological niches. We aimed to isolate staphylococci from owls and to characterize their antimicrobial resistance, virulence factors and ...
High frequency of the EMRSA-15 clone (ST22-MRSA-IV) in hospital wastewater
Silva, Vanessa; Ribeiro, Jessica; Rocha, Jaqueline; Manaia, Célia M.; Silva, Adriana; Pereira, José Eduardo; Maltez, Luís; Capelo, José LuisHospital wastewaters often carry multidrug-resistant bacteria and priority pathogens, such as methicillin-resistant Staphylococcus aureus (MRSA). Pathogens and antibiotic resistance genes present in wastewaters may reach the natural environment facilitating their spread. Thus, we aimed to isolate MRSA from wastewater of 3 hospitals located in the north of Portugal and to characterize the isolates regarding the ...
Subproteomic signature comparison of in vitro selected fluoroquinolone resistan...
Correia, Susana; Hébraud, Michel; Chafsey, Ingrid; Chambon, Christophe; Viala, Didier; Torres, Carmen; Caniça, Manuela; Capelo, José LuisBackground: Fluoroquinolone resistance in nontyphoidal Salmonella is a situation of serious and international concern, particularly in S. Typhimurium DT104B multiresistant strains. Although known to be multifactorial, fluoroquinolone resistance is still far from a complete understanding. Methods: Subproteome changes between an experimentally selected fluoroquinolone-resistant strain (Se6-M) and its parent strai...
An unusual highly emissive water-soluble iridium lissamine-alanine complex and ...
Oliveira, Elisabete; Santos, Sérgio M.; Núñez, Cristina; Capelo, José Luis; Lodeiro, CarlosThe interaction of iridium(III) with a new lissamine rhodamine B sulfonyl derivative, bearing alanine as a building block, (1) with an orange emission in water results in a green highly emissive Ir@1 complex at room temperature. The new Ir@1 complex can sense the toxic Hg2+ metal ion and cysteine. Based on such properties, a new sophisticated molecular logic gate with three inputs was designed.
Combined proteomic and metallomic analyses in Scrobicularia plana clams to asse...
Gonzalez-Dominguez, Raúl; Santos, Hugo Miguel; Bebianno, Maria João; Garcia-Barrera, Tamara; Gomez-Ariza, José Luis; Capelo, José LuisEstuaries are very important ecosystems with great ecological and economic value, but usually highly impacted by anthropogenic pressure. Thus, the assessment of pollution levels in these habitats is critical in order to evaluate their environmental quality. In this work, we combined complementary metallomic and proteomic approaches with the aim to monitor the effects of environmental pollution on Scrobicularia ...
From yellow to pink using a fluorimetric and colorimetric pyrene derivative and...
Pinheiro, Daniela; Castro, Catherine S. de; Melo, J. Sérgio Seixas de; Oliveira, Elisabete; Nuñez, Cristina; Fernández-Lodeiro, Adrián1st International Caparica Conference on Chromogenic and Emissive Materials; A new simple and multifunctional fluorescent probe based on pyrene linked through an imine bond to a hydroxyphenol unit was synthesised, fully characterized and investigated in solution. Additionally, its behaviour in the presence of several ions (H+, Zn2+, Cd2+, Cu2+, Ni2+, Pb2+, Fe2+, Hg2+ and Al3+) was investigated in CH2Cl2. It was...
A new approach to bacterial colony morphotyping by matrix-assisted laser desorp...
Sousa, Ana Margarida; Nunes-Miranda, J. D.; Reboiro-Jato, M.; Fdez-Riverola, Florentino; Lourenço, Anália; Pereira, Maria Olívia; Capelo, José LuisMatrix assisted laser desorption ionization time of flight mass spectrometry has been explored as a tool to bacterial colony morphotyping.To this end,four colony morphotypes of Pseudomonas aeruginosa and four of Staphylococcus aureus were analysed using intact bacteria.Results suggest that mass spectrometry of intact bacteria could,in some extent,be used to complement the classical morphological classification ...
Timely and effectively profile bacteria in cystic fibrosis lungs
Sousa, A. M.; Lourenço, Anália; Fdez-Riverola, Florentino; Diniz, Júlio; Capelo, José Luis; Pereira, Maria OlíviaBacterial lung infections are typical of cystic fibrosis (CF) disease due to accumulation of airway mucus. Despite the use of aggressive antibiotic therapy, the mortality rate of CF patients is still high. Unsuccessful bacterial eradication is often due to several evolutional strategies adopted by bacteria to achieve anaerobic or microaerophilic adaptation and antibiotic resistance, such as biofilm formation an...
Synthesis, characterization, photophysical studies and interaction with DNA of ...
Pedras, Bruno; Batista, Rosa Maria Ferreira; Tormo, Laura; Costa, Susana P. G.; Raposo, M. Manuela M.; Orellana, Guillermo; Capelo, José LuisA new family of Ru(II) polypyridyl complexes (C1 to C6) containing furyl- or thienyl-imidazo-phenanthroline ligands (4-6) were synthesized using microwave irradiation and characterized by elemental analysis, 1H-NMR, UV-vis absorption and fluorescence spectroscopy, FAB, EI-MS and MALDI-TOF-MS spectrometry. On the other hand, the novel furyl- or thienyl-imidazo-phenanthroline derivatives (5-6) were synthesized th...