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Dietary natural plant extracts can promote growth and modulate oxidative status...

Xavier, Maria João; Conceição, Luís E. C.; Valente, Luisa M. P.; Colen, R.; Rodrigues, Andreia C. M.; Rocha, Rui J. M.; L, Custódio; Carballo, Carlos

Plant extracts are known for their high content and diversity of polyphenols, which can improve fish oxidative status. A growth trial with Senegalese sole postlarvae (45 days after hatching) fed with one of four experimental diets—control (CTRL), and supplemented with curcumin (CC), green tea (GT), and grape seed (GS) extracts—was performed to assess if supplementation could improve growth performance and oxida...


Dietary antioxidant supplementation promotes growth in senegalese sole postlarvae

Xavier, Maria J; Engrola, Sofia; Conceição, Luis E C; Manchado, Manuel; Carballo, Carlos; Gonçalves, Renata; Colen, R.; Figueiredo, Vera

Somatic growth is a balance between protein synthesis and degradation, and it is largely influenced by nutritional clues. Antioxidants levels play a key role in protein turnover by reducing the oxidative damage in the skeletal muscle, and hence promoting growth performance in the long-term. In the present study, Senegalese sole postlarvae (45 days after hatching, DAH) were fed with three experimental diets, a c...


Microalgal extracts induce larval programming and modify growth and the immune ...

Carballo, Carlos; Mateus, Ana; Maya, Claudia; Mantecon, Lalia; Power, Deborah; Manchado, Manuel

Immunostimulants are key molecules in aquaculture since they heighten defensive responses and protection against pathogens. The present study investigated the treatment of Senegalese sole larvae with a whole-cell crude extract of the microalgae Nannochloropsis gaditana (Nanno) and programming of growth and the immune system. Larvae at hatch were treated with the Nanno extracts for 2 h and thereafter were cultiv...


Yeast β-glucans and microalgal extracts modulate the immune response and gut mi...

Carballo, Carlos; Pinto, Patricia IS; Mateus, Ana; Berbel, Concha; Guerreiro, Claudia; Martinez-Blanch, Juan F.; Codoñer, Francisco M.; Mantecon, Lalia

One bottleneck to sustainability of fish aquaculture is the control of infectious diseases. Current trends include the preventive application of immunostimulants and prebiotics such as polysaccharides. The present study investigated how yeast β-glucan (Y), microalgal polysaccharide-enriched extracts (MAe) and whole Phaeodactylum tricornutum cells (MA) modulated the gut microbiome and stimulated the immune syste...


Understanding pseudo-albinism in sole (Solea senegalensis): a transcriptomics a...

Pinto, Patricia IS; Guerreiro, Claudia; Costa, Rita A.; Martinez-Blanch, Juan F.; Carballo, Carlos; Codoñer, Francisco M.; Manchado, Manuel

Pseudo-albinism is a pigmentation disorder observed in flatfish aquaculture with a complex, multi-factor aetiology. We tested the hypothesis that pigmentation abnormalities are an overt signal of more generalised modifications in tissue structure and function, using as a model the Senegalese sole and two important innate immune barriers, the skin and intestine, and their microbiomes. Stereological analyses in p...


Phylogeny, expression patterns and regulation of DNA Methyltransferases in earl...

Firmino, Joana; Carballo, Carlos; Armesto, Paula; Campinho, Marco António; Power, Deborah M.; Manchado, Manuel

Background: The identification of DNA methyltransferases (Dnmt) expression patterns during development and their regulation is important to understand the epigenetic mechanisms that modulate larval plasticity in marine fish. In this study, dnmt1 and dnmt3 paralogs were identified in the flatfish Solea senegalensis and expression patterns in early developmental stages and juveniles were determined. Additionally,...


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