7 documents found, page 1 of 1

Agro-industrial wastes as alternative substrates for the production of prebioti...

Fructooligosaccharides (FOS) are promising prebiotics in the relevant and increasing market of functional food. However, to achieve a more sustainable process, the industrial production of FOS should use cheap substrates. Zymomonas mobilis (ZM) has great potential for the production of FOS due to the presence of native enzymes (levansucrase) capable of metabolizing sucrose. In addition, ZM can use different car...

Novel enzyme-based bioprocess to produce the prebiotic epilactose

A epilactose é um oligossacárido funcional raro e ainda muito pouco explorado. Esta tese teve como objetivo desenvolver um novo bioprocesso enzimático para a produção de epilactose, bem como a sua purificação e avaliação do seu potencial efeito prebiótico. A levedura Saccharomyces cerevisiae foi usada como hospedeiro para a produção heteróloga da enzima celobiose 2-epimerase da bactéria Caldicellulosiruptor sac...

Assessing the prebiotic potential of xylooligosaccharides produced by one-step ...

The prebiotic effect is a fundamental concept in the fields of nutrition and gut health, referring to the beneficial effects of specific non-digestible dietary components on the gut microbiota, including xylooligosaccharides (XOS). These compounds function as food sources for beneficial gut bacteria, fostering their growth and activity. In this work, in vitro studies were performed to evaluate the prebiotic pot...

Development of a cost-effective media for biosurfactants production by Pseudomo...

In the last years, the textile industry has shown a growing interest in biosurfactants due to their biocompatibility , biodegradability , and versatility at various pH and temperature ranges . These compounds have found applications as softeners, wetting agents, lubricants, foam stabilizers, and even in the scouring of wool. This study aims to develop an economically efficient medium for biosurfactant productio...

Zymomonas mobilis an emerging microbial cell factory to produce prebiotics

Epilactose biosynthesis using recombinant cellobiose 2-epimerase produced by Sa...

Cellobiose 2-epimerases are interesting enzymes that can catalyze the production of lactose-based prebiotics, namely, epilactose and lactulose, through a single substrate reaction with noteworthy yields. In this work, the GRAS yeast Saccharomyces cerevisiae was for the first time used as a host for the heterologous production of cellobiose 2-epimerase from Caldicellulosiruptor saccharolyticus. The enzyme showed...

Validation and optimization of β-galactosidasev production by Aspergillus lacti...

β-galactosidases (EC 3.2.1.23), also known as lactases, are a family of enzymes that are able to catalyze two different reactions, namely the hydrolysis of lactose and transgalactosylation. The hydrolytic activity is commonly applied in the food industries for reducing the lactose content on dairy products, but it is also important to prevent lactose crystallization problems and to increase the sweetening capac...