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Improved dsRNA isolation and purification method validated by viral dsRNA detec...

Cardoso, Fernando M.H.; Elias, Alexandre; Pereira, Inês; Maurício, Isabel; Matos, Olga

Accurate genomic sequencing demands high-quality double-stranded RNA (dsRNA). Existing methods for dsRNA extraction from yeast, fungi, and plants primarily rely on cellulose, suitable only for small volume extractions, or the time-consuming lithium chloride precipitation. To streamline the traditional phenol-chloroform-based dsRNA extraction method, the main challenge is the reduction of mitochondrial DNA (mtDN...

Date: 2023   |   Origin: Repositório da Universidade de Lisboa
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