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Impact of seasons on industrial cultivation of Limnospira platensis (Spirulina)...

Guerra, Inês; Torres, Cátia; Cardoso, Helena; Pereira, Hugo; Trovão, Mafalda; Rodrigues, Alexandre M. C.; Varela, João

Spirulina is the most commercially produced biomass, among microalgae and cyanobacteria, with extensive applications across food, feed, nutraceutical and biotechnological sectors. While Spirulina cultivation is wellcharacterized at laboratorial and small-scale production, critical knowledge gaps remain regarding industrialscale production in temperate climates. Specifically, there is a lack of robust empirical ...


Heterotrophic cultivation of Chlorella vulgaris yellow mutant on sidestreams: M...

Trovão, Mafalda; Barros, Ana; Machado, Adriana; Reis, Ana; Pedroso, Humberto; Espírito Santo, Gonçalo; Correia, Nádia; Costa, Monya; Ferreira, Sara

Microalgal protein is a promising feedstock to complement and/or replace other protein sources. Besides requiring less land and water usage, microalgae production is a more sustainable process, especially if industrial sidestreams are used as nutrient sources. Additionally, the heterotrophic cultivation of microalgae, such as Chlorella vulgaris, enables the achievement of much higher biomass productivity and lo...


Comparative analysis of isoprostanoid profiles in Chlorella sorokiniana grown u...

Conde, Tiago; Lopes, Diana; Gros, Valérie; Reversat, Guillaume; Oger, Camille; Galano, Jean-Marie; Vigor, Claire; Rodrigues, Alexandre M. C.

Oxylipins are bioactive lipid mediators derived from polyunsaturated fatty acids (PUFAs), with roles in oxidative stress responses, immunomodulation, and inflammation. While microalgae are recognized as valuable sources of oxylipins, their profiles remain less studied across different species and cultivation conditions. In this study, we characterized the non-enzymatic oxylipin profile of Chlorella sorokiniana ...


Insight into the efficiency of microalgae’ lipidic extracts as photosensitizers...

Mendonça, Inês; Silva, Daniela; Conde, Tiago; Maurício, Tatiana; Cardoso, Helena; Pereira, Hugo; Bartolomeu, Maria; Vieira, Cátia; Domingues, M. Rosário

Antibacterial resistance causes around 1.27 million deaths annually around the globe and has been recognized as a top 3 priority health threat. Antimicrobial photodynamic therapy (aPDT) is considered a promising alternative to conventional antibiotic treatments. Algal lipid extracts have shown antibacterial effects when used as photosensitizers (PSs) in aPDT. In this work we assessed the photodynamic efficiency...


Nonthermal high-pressure microalgae extracts: A new source of natural ingredien...

Garcia, Mónica P.; Regueiras, Ana; Lopes, Graciliana; Matos, Gabriela; Silva, Lucília P. da; Cerqueira, Mariana T.; Cardoso, Helena; Correia, Nádia

Microalgae are one of the most prominent sources of ingredients for the cosmetic industry not only due to their diversity but, importantly, due to the low environmental impact in production and extraction. A sustainable extraction process requires the use of effective and environmentally friendly solvents that guarantee an extract with a relevant content and in high yields. In this work, Chlorella vulgaris, Nan...


Oxyfluorfen: a novel metabolic inhibitor to select microalgal chlorophyll-defic...

Trovão dos Santos, Mafalda; Cardoso, Lucas; Schüler, Lisa; Machado, Adriana; Santo, Gonçalo Espírito; Pedroso, Humberto; Reis, Ana; Barros, Ana

Nowadays, there is an increasing demand for novel feedstocks and alternative protein sources to meet global needs. Because of their rich nutritional profiles and high protein contents, microalgae-based food products and supplements are being developed. Nonetheless, these products present organoleptic characteristics such as taste, smell and colour that are often considered unpleasant by human and animal consume...


Insight into the efficiency of microalgae’ lipidic extracts as photosensitizers...

Mendonça, Inês; Silva, Daniela; Conde, Tiago; Maurício, Tatiana; Cardoso, Helena; Pereira, Hugo; Bartolomeu, Maria; Vieira, Cátia; Domingues, M. Rosário

Antibacterial resistance causes around 1.27 million deaths annually around the globe and has been recognized as a top 3 priority health threat. Antimicrobial photodynamic therapy (aPDT) is considered a promising alternative to conventional antibiotic treatments. Algal lipid extracts have shown antibacterial effects when used as photosensitizers (PSs) in aPDT. In this work we assessed the photodynamic efficiency...


Isolation and selection of protein-rich mutants of chlorella vulgaris by fluore...

Trovão dos Santos, Mafalda; Schüler, Lisa; Pedroso, Humberto; Reis, Ana; Santo, Gonçalo Espírito; Barros, Ana; Correia, Nádia; Ribeiro, Joana

Microalgae are a promising feedstock with proven biostimulant activity that is enhanced by their biochemical components (e.g., amino acids and phytohormones), which turns them into an appealing feedstock to reduce the use of fertilisers in agriculture and improve crop productivity and resilience. Thus, this work aimed to isolate protein-rich microalgal mutants with increased biostimulant activity. Random mutage...


Microalgae as a selenium vehicle for nutrition: a review

Pires, Rita; Costa, Margarida; Pereira, Hugo; Cardoso, Helena; Ferreira, Luís; Lapa, Nuno; Silva, Joana; Ventura, Márcia

Selenium (Se) is essential for human and animal nutrition, playing a key role in antioxidant and immune functions. Organic Se is better for supplementation because it is more efficiently assimilated and less toxic than its inorganic form. Due to the scarcity of Se in European soils, supplementation in feed and food is necessary. Currently, inorganic Se (sodium selenite and selenate) and organic Se in Se-enriche...


Benchmarking computer-vision-based facial emotion classification algorithms whi...

Coelho, Luis; Reis, Sara; Moreira, Cristina; Cardoso, Helena; Sequeira, Miguela; Coelho, Raquel

Effective human communication relies heavily on emotions, making them a crucial aspect of interaction. As technology progresses, the desire for machines to exhibit more human-like characteristics, including emotion recognition, grows. DeepFace has emerged as a widely adopted library for facial emotion recognition. However, the widespread use of surgical masks after the COVID-19 pandemic presents a considerable ...


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