11 documents found, page 1 of 2
Transcriptome analysis reveals the high ribosomal inhibitory action of 1,4-naph...
Cardoso, Joana M. S.; Esteves, Ivânia; Egas, Conceição; Braga, Mara E. M.; Sousa, Herminio C. de; Abrantes, Isabel; Maleita, CarlaThe root-knot nematode (RKN) Meloidogyne luci presents a threat to the production of several important crops. This nematode species was added to the European Plant Protection Organization Alert list in 2017. The scarce availability of efficient nematicides to control RKN and the phasing out of nematicides from the market have intensified the search for alternatives, such as phytochemicals with bionematicidal pr...
Potensaphelenchus stammeri (Körner, 1954) Gu, Liu, Abolafia & Pedram, 2021 (Nem...
Silva, Hugo; Cardoso, Joana M. S.; Costa, Ricardo M. F. da; Abrantes, Isabel; Fonseca, LuísIn a survey for Burspahelenchus species in a declining centennial stone pine, Pinus pinea, in Portugal, few specimens of Potensaphelenchus stammeri were extracted from wood samples, and an isolate was established in fungus cultures. The Portuguese P. stammeri isolate was characterised and identified based on morphological and morphometric diagnostic characters of females and males and by sequencing the D2-D3 ex...
Virulence Biomarkers of Bursaphelenchus xylophilus: A Proteomic Approach
Cardoso, Joana M. S.; Anjo, Sandra I.; Manadas, Bruno; Silva, Hugo; Abrantes, Isabel M. O.; Nakamura, Katsunori; Fonseca, LuísThe pinewood nematode (PWN), Bursaphelenchus xylophilus, one of the most serious forest pests worldwide, is considered the causal agent of the pine wilt disease (PWD). The main host species belong to the genus Pinus, and a variation in the susceptibility of several pine species to PWN infection is well-known. It is also recognized that there is variation in the virulence among B. xylophilus isolates. In the pre...
Pratylenchus penetrans Parasitizing Potato Crops: Morphometric and Genetic Vari...
Gil, Diogo; Cardoso, Joana M. S.; Abrantes, Isabel M. O.; Esteves, Ivânia Sofia GrasinaThe root lesion Pratylenchus penetrans is an economically important pest affecting a wide range of plants. The morphometry of five P. penetrans isolates, parasitizing potato roots in Portugal, was compared and variability within and between isolates was observed. Of the 15 characters assessed, vulva position (V%) in females and the stylet length in both females/males showed the lowest coefficient of intra and i...
Comparative Analysis of Bursaphelenchus xylophilus Secretome Under Pinus pinast...
Silva, Hugo; Anjo, Sandra I.; Manadas, Bruno; Abrantes, Isabel; Fonseca, Luís; Cardoso, Joana M. S.The pinewood nematode (PWN), Bursaphelenchus xylophilus, the pine wilt disease's (PWD) causal agent, is a migratory endoparasitic nematode skilled to feed on pine tissues and on fungi that colonize the trees. In order to study B. xylophilus secretomes under the stimulus of pine species with different susceptibilities to disease, nematodes were exposed to aqueous pine extracts from Pinus pinaster (high-susceptib...
Species-Specific Molecular Detection of the Root Knot Nematode Meloidogyne luci
Maleita, Carla; Cardoso, Joana M. S.; Rusinque, Leidy; Esteves, Ivânia; Abrantes, IsabelMeloidogyne luci has been identified in various countries around the world parasitizing economically important crops and, due to its potential to cause serious damage to agriculture, was included in the European and Mediterranean Plant Protection Organization Alert List in 2017. This species shares morphological and molecular similarities with M. ethiopica and M. inornata, and a M. ethiopica group was therefore...
α-l-Fucosidases from Bursaphelenchus xylophilus Secretome—Molecular Characteriz...
Cardoso, Joana M. S.; Fonseca, Luís; Abrantes, IsabelThe pinewood nematode (PWN) Bursaphelenchus xylophilus, the causal agent of the pine wilt disease (PWD), enters above-ground parts of the tree, migrates through the resin canals and feeds on plant cells causing extensive damage. In order to penetrate the cell wall and establish a parasitic relationshipwith host trees, the PWNneeds to secreteamixture of active cellwall degrading enzymes. In maritime pine, Pinus ...
Bursaphelenchus xylophilus and B. mucronatus secretomes: a comparative proteomi...
Cardoso, Joana M. S.; Anjo, Sandra I.; Fonseca, Luís; Egas, Conceição; Manadas, Bruno; Abrantes, IsabelThe pinewood nematode, Bursaphelenchus xylophilus, recognized as a worldwide major forest pest, is a migratory endoparasitic nematode with capacity to feed on pine tissues and also on fungi colonizing the trees. Bursaphelenchus mucronatus, the closest related species, differs from B. xylophilus on its pathogenicity, making this nematode a good candidate for comparative analyses. Secretome profiles of B. xylophi...
The complete genome sequence of a new necrovirus isolated from Olea europaea L.
Cardoso, Joana M. S.; Félix, M. Rosário; Clara, M. Ivone; Oliveira, SolangeThe complete nucleotide sequence of a virus isolated from Olea europaea L. (GP isolate), previously identified as an isolate of Tobacco necrosis virus D (TNV-D) based on its coat protein sequence, was determined. The viral RNA genome consists of 3683 nucleotides and contains five open reading frames. The putative RNA-dependent RNA polymerase shows 91.2% amino acid identity with that of an isolate of Olive laten...
Viral properties, primary structure and phylogenetic analysis of the coat prote...
Félix, M. Rosário; Cardoso, Joana M. S.; Oliveira, Solange; Clara, M. IvoneAn Olive latent virus 1 isolate designated GM6, obtained from a Portuguese olive tree, was characterized and the coat protein gene sequenced and analysed. The purified virus particles showed to be isometric with ca.30 nm in diameter and contained a single-stranded RNA species with ca. 3.7 kb. The dsRNA profile obtained from infected tissues showed three major species with ca. 3.7, 1.5 and 1.3 kbp. SDS-PAGE anal...