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Affordable Pretreatment Strategy for Mitigation of Biofouling in Drinking-Water...

Luis F. Melo; Ana C. Barros; Ana Alexandra Pereira; Idalina Machado

Biofouling triggers a chain of events that are deleterious to engineered systems providing safe water to the population and industry. The frequent use of dissolved biocides to combat biofilm growth has its own drawbacks, including the formation of noxious substances (e.g., organochlorinated compounds) and the discharge of significant amounts of nonspent biocides in the aquatic environment. This paper proposes a...



Pseudomonas fluorescens tolerance to benzyldimethyldodecyl ammonium chloride: a...

Maria Sousa Silva; Manuel Simões; Luís Melo; Idalina Machado

Objectives: Benzyldimethyldodecyl ammonium chloride (BDMDAC) is a quaternary ammonium compound (QAC) with bactericidal action that is used as an active molecule in detergent formulations. Pseudomonas fluorescens is a Gram-negative bacterium with versatile metabolism that is frequently present in biofilms on industrial surfaces. This work reports P. fluorescens adaptation to BDMDAC and subsequent concurrent redu...


Characterization of the heterotrophic bacteria from a minimally processed veget...

Ana Meireles; Rita Fulgêncio; Idalina Machado; Filipe Mergulhão; L. F. Melo; Manuel Simões

The knowledge on the microorganisms present in an industrial process is crucial to delineate the beststrategy for their effective control. The aims of the present work were to isolate, identify and characterize(in terms of production of proteases, gelatinases and siderophores, quorum-sensing inhibition andbiofilm formation) the resident heterotrophic bacteria present in a minimally processed vegetables(MPV) pla...


Combination of selected enzymes with cetyltrimethylammonium bromide in biofilm ...

Paula A. Araújo; Idalina Machado; Ana Meireles; TorOve Leiknes; Filipe Mergulhão; Luís F. Melo; Manuel Simões

Enzymes are considered an innovative and environmentally friendly approach for biofilm control due to their lytic and dispersal activities. In this study, four enzymes (β-glucanase, α-amylase, lipase and protease) were tested separately and in combination with the quaternary ammonium compound cetyltrimethylammonium bromide (CTAB) to control flow-generated biofilms of Pseudomonas fluorescens. The four enzymes ...


Deciphering the contribution of biofilm to the pathogenesis of peritoneal dialy...

Joana Sampaio; Diana Machado; Ana Marta Gomes; Idalina Machado; Cledir Santos; Nelson Lima; Maria João Carvalho; António Cabrita; Anabela Rodrigues

Infections are major complications in peritoneal dialysis (PD) with a multifactorial etiology that comprises patient, microbial and dialytic factors. This study aimed at investigating the contribution of microbial biofilms on PD catheters to recalcitrant infections and their interplay with PD related-factors. A prospective observational study was performed on 47 patients attending Centro Hospitalar of Porto and...


Influence of flow velocity on the characteristics of pseudomonas fluorescens bi...

Paula A. Araújo; Joana Malheiro; Idalina Machado; Filipe Mergulhão; Luís Melo; Manuel Simões

The characteristics of Pseudomonas fluorescens biofilms formed under three different linear flow velocities (u=0.1, 0.4, and 0.8 m/s; Reynolds numbers of 1,000, 4,000, and 8,000, respectively) were studied providing an extension to the studies of earlier researchers. A flow cell reactor system was used to form biofilms, and they were characterized in terms of thickness, morphological structure, mass, cell densi...


Biofilms7 - microbial works of art

Filipe Mergulhão; L. F. Melo; Simões M; Azevedo, NF; Azeredo, J; Pereira, MO; Henriques, M; Cerca, N; Idalina Machado


Efficacy of antimicrobial combinations to reduce the use of sodium hypochlorite...

Ana Meireles; Idalina Machado; Rita Fulgêncio; Filipe Mergulhão; Luís Melo; Manuel Simões

Foodborne illness outbreaks linked to fresh produce are becoming more frequent and widespread. The types and properties of the chemical agents used for washing, cleaning and disinfection procedures, particularly their toxicity are the key indicators of environmental performance of a minimally processed vegetables (MPV) industry. The main aims of this work were focused on the evaluation of selected disinfectants...


BiofOmics: A Web Platform for the Systematic and Standardized Collection of Hig...

Anália Lourenço; Andreia Ferreira; Nuno Veiga; Idalina Machado; Maria Olivia Pereira; Nuno F. Azevedo

Background: Consortia of microorganisms, commonly known as biofilms, are attracting much attention from the scientific community due to their impact in human activity. As biofilm research grows to be a data-intensive discipline, the need for suitable bioinformatics approaches becomes compelling to manage and validate individual experiments, and also execute inter-laboratory large-scale comparisons. However, bio...


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