11 documents found, page 1 of 2
Signature cytokine-associated transcriptome analysis of effector γδ T cells ide...
Inácio, Daniel; Amado, Tiago; Pamplona, Ana; Sobral, Daniel; Cunha, Carolina; Santos, Rita F.; Oliveira, Liliana; Rouquié, Nelly; Carmo, Alexandre M.γδ T cells producing either interleukin-17A (γδ17 cells) or interferon-γ (γδIFN cells) are generated in the mouse thymus, but the molecular regulators of their peripheral functions are not fully characterized. Here we established an Il17a-GFP:Ifng-YFP double-reporter mouse strain to analyze at unprecedented depth the transcriptomes of pure γδ17 cell versus γδIFN cell populations from peripheral lymph nodes. Wit...
Signature cytokine-associated transcriptome analysis of effector γδ T cells ide...
Inácio, Daniel; Amado, Tiago; Pamplona, Ana; Sobral, Daniel; Cunha, Carolina; Santos, Rita F.; Oliveira, Liliana; Rouquié, Nelly; Carmo, Alexandre M.γδ T cells producing either interleukin-17A (γδ cells) or interferon-γ (γδ cells) are generated in the mouse thymus, but the molecular regulators of their peripheral functions are not fully characterized. Here we established an Il17a-GFP:Ifng-YFP double-reporter mouse strain to analyze at unprecedented depth the transcriptomes of pure γδ cell versus γδ cell populations from peripheral lymph nodes. Within a very...
CD5L is upregulated upon infection with Mycobacterium tuberculosis with no effe...
Cardoso, Marcos S.; Gonçalves, Rute; Oliveira, Liliana; Silvério, Diogo; Téllez, Erica; Paul, Tony; Sarrias, Maria Rosa; Carmo, Alexandre M.Tuberculosis (TB) alone caused over a billion deaths in the last 200 years, making it one of the deadliest diseases to humankind. Understanding the immune mechanisms underlying protection or pathology in TB is key to uncover the much needed innovative approaches to tackle TB. The scavenger receptor cysteine-rich molecule CD5 antigen-like (CD5L) has been associated with TB, but whether and how CD5L shapes the im...
Neutral PEGylated liposomal formulation for efficient folate-mediated delivery ...
Nogueira, E.; Freitas, Jaime; Loureiro, Ana Isabel Sá; Nogueira, Patrícia; Gomes, Andreia C; Preto, Ana; Carmo, Alexandre M.; Moreira, AlexandraCationic liposomes are efficient vectors for systemic delivery of therapeutic small interfering RNA (siRNA), taking advantage of RNA interference (RNAi), a naturally occurring gene-silencing mechanism in mammalian cells. However, toxicity at high concentrations, short circulating half-lives and lack of specificity restrict their successful application in a wider scale. The purpose of this study was to evaluate ...
Assessment of liposome disruption to quantify drug delivery in vitro
Nogueira, E.; Cruz, Célia F.; Loureiro, A.; Nogueira, Patrícia; Freitas, Jaime; Moreira, Alexandra; Carmo, Alexandre M.; Gomes, Andreia C; Preto, AnaEfficient liposome disruption inside the cells is a key for success with any type of drug delivery system. The efficacy of drug delivery is currently evaluated by direct visualization of labeled liposomes internalized by cells, not addressing objectively the release and distribution of the drug. Here, we propose a novel method to easily assess liposome disruption and drug release into the cytoplasm. We propose ...
Peptide anchor for folate-targeted liposomal delivery
Nogueira, E.; Mangialavori, Irene C.; Loureiro, Ana; Azóia, Nuno G.; Sarria, Marisa P.; Nogueira, Patrícia; Freitas, Jaime; Harmark, JohanSpecific folate receptors are abundantly overexpressed in chronically activated macrophages and in most cancer cells. Directed folate receptor targeting using liposomes is usually achieved using folate linked to a phospholipid or cholesterol anchor. This link is formed using a large spacer like polyethylene glycol. Here, we report an innovative strategy for targeted liposome delivery that uses a hydrophobic fra...
Enhancing methotrexate tolerance with folate tagged liposomes in arthritic mice
Nogueira, Eugénia Sofia Costa; Lager, Franck; Le Roux, Delphine; Nogueira, Patrícia; Freitas, Jaime; Charvet, Celine; Renault, Gilles; Loureiro, AnaMethotrexate is the first line of treatment of rheumatoid arthritis. Since many patients become unresponsive to methotrexate treatment, only very expensive biological therapies are effective and increased methotrexate tolerance strategies need to be identified. Here we propose the encapsulation of methotrexate in a new liposomal formulation using a hydrophobic fragment of surfactant protein conjugated to a link...
T cell activation regulates CD6 alternative splicing by transcription dynamics ...
Araújo, Mafalda Martins de; Glória, Vânia G. da; Santos, Ana Mafalda; Leal, Rafaela; Almeida, Sérgio F. de; Carmo, Alexandre M.; Moreira, AlexandraThe T cell-surface glycoprotein CD6 is a modulator of cellular responses and has been implicated in several autoimmune diseases such as multiple sclerosis, rheumatoid arthritis, and psoriasis. During Ag presentation, CD6 is targeted to the immunological synapse in a ligand binding-dependent manner, in which CD6 domain 3 directly contacts CD166, expressed on the APC. T cell activation results in the induction of...
T cell activation regulates CD6 alternative splicing by transcription dynamics ...
da Glória, Vânia G.; Martins de Araújo, Mafalda; Mafalda Santos, Ana; Leal, Rafaela; de Almeida, Sérgio F.; Carmo, Alexandre M.; Moreira, AlexandraThe T cell-surface glycoprotein CD6 is a modulator of cellular responses and has been implicated in several autoimmune diseases such as multiple sclerosis, rheumatoid arthritis, and psoriasis. During Ag presentation, CD6 is targeted to the immunological synapse in a ligand binding-dependent manner, in which CD6 domain 3 directly contacts CD166, expressed on the APC. T cell activation results in the induction of...
Liposome and protein based stealth nanoparticles
Nogueira, Eugénia Sofia Costa; Loureiro, Ana; Nogueira, Patrícia; Freitas, Jaime; Almeida, Catarina R.; Harmark, Johan; Hebert, Hans; Moreira, AlexandraLiposomes and protein based nanoparticles were tuned with different polymers and glycolipids to improve stealth and thus decrease their clearance by macrophages. Liposomes were coated with polyethylene glycol (PEG) and brain-tissue-derived monosialoganglioside (GM1). Bovine serum albumin (BSA) nanoparticles were produced incorporating a PEGylated surfactant (PEG-surfactant). All obtained nanoparticles were mono...