6 documents found, page 1 of 1
Prevalência de conjuntivite adenoviral em clínica oftalmológica no município de...
Rosado-Filho, Euldes Nei; Cardoso, Silvia Almeida; Marçal, Lorena Nacif; Santos, Eliziária Cardoso dos; Silva, Eduardo de Almeida Marques daAvaliar a prevalência de Adenovírus como agente etiológico da conjuntivite, em clínica médica oftalmológica especializada, em Viçosa, Minas Gerais, Brasil. Amostras da secreção conjuntival de 91 pacientes clinicamente diagnosticados com conjuntivite foram submetidos à reação em cadeia da polimerase (PCR), utilizando primers degenerados para a região codificadora do gene da proteína estrutural II. Posteriormente...
Prevalence of vitamin D deficiency and its relationship with factors associated...
Peçanha, Mirna Brito; Freitas, Rodrigo de Barros; Moreira, Tiago Ricardo; Silva, Luiz Sérgio; Oliveira, Leandro Licursi de; Cardoso, Silvia AlmeidaTo determine the prevalence of vitamin D deficiency/insufficiency in children 0-18 years of age with recurrent wheezing and/or asthma residing in the microregion of Viçosa, Minas Gerais, Brazil, and treated at a referral center, and to determine its association with major risk factors for wheezing. A cross-sectional study was performed using a semi-structured questionnaire, which was administered by trained int...
Construction of recombinant Kluyveromyces marxianus UFV-3 to express dengue vir...
Bragança, Caio Roberto Soares; Colombo, Lívia Tavares; Roberti, Alvaro Soares; Alvim, Mariana Caroline Tocantins; Cardoso, Silvia AlmeidaThe yeast Kluyveromyces marxianus is a convenient host for industrial synthesis of biomolecules. However, despite its potential, there are few studies reporting the expression of heterologous proteins using this yeast. Here, we report expression of a dengue virus protein in K. marxianus for the first time. The dengue virus type 1 nonstructural protein 1 (NS1) was integrated into the K. marxianus UFV-3 genome at...
Bacteriophage isolated from sewage eliminates and prevents the establishment of...
Ribeiro, Karla Veloso Gonçalves; Ribeiro, Cleberson; Dias, Roberto Sousa; Cardoso, Silvia Almeida; Paula, Sérgio Oliveira de; Zanuncio, Jose ColaBiofilm growth exerts a negative impact on industry and health, necessitating the development of strategies to control. The objective of this work was study the lytic activity of the phage isolated from the sewage network in the formation and degradation of Escherichia coli biofilms. E. coli cultures were incubated in 96-well polystyrene microplates under controlled conditions to evaluate the biofilm formation....
Exposure to deltamethrin induces oxidative stress and decreases of energy reser...
Oliveira, Jerusa Maria; Losano, Nicole Fontes; Condessa, Suellen Silva; Freitas, Renata Maria Pereira de; Cardoso, Silvia AlmeidaDeltamethrin (DTM) is a synthetic pyrethroid insecticide highly used by farmers and home users. This pesticide has lipophilic properties that facilitate a high absorption and can cause toxicity in non-target organisms. During foraging, the fruit-eating bats Artibeus lituratus are exposed to pesticides. However, the knowledge of the toxicity of pesticides on the physiology of bats is relatively scarce. This stud...
Nasal vaccination with attenuated Salmonella expressing VapA: TLR2 activation i...
Cardoso, Silvia Almeida; Oliveira, Aline Ferreira; Ruas, Luciana Pereira; Trevisani, Marcel Montels; Oliveira, Leandro Licursi De; Hanna, Ebert SeixasVirulent strains of Rhodococcus equi have a large plasmid of 80–90 kb, which encodes several virulence-associated proteins (Vap), including VapA, a lipoprotein highly associated with disease. We have previously demonstrated that oral immunisation with attenuated Salmonella enterica Typhimurium strain expressing the antigen VapA (STM VapA+) induces specific and long-term humoral and cellular immunity against R. ...