6 documents found, page 1 of 1
Mycobacterium tuberculosis strains are differentially recognized by TLRs with a...
Carmona, J. A.; Cruz, Andrea; Teixeira, Lucia de Fatima Moreira; Sousa, Carole Lara Veiga de; Sousa, Jeremy Nicolas Carvalho; Osório, Nuno S.Tuberculosis associates with a wide spectrum of disease outcomes. The Beijing (Bj) lineage of Mycobacterium tuberculosis (Mtb) is suggested to be more virulent than other Mtb lineages and prone to elicit non-protective immune responses. However, highly heterogeneous immune responses were reported upon infection of innate immune cells with Bj strains or stimulation with their glycolipids. Using both in vitro and...
Influence of the variability of Mycobacterium tuberculosis strains on the host ...
Carmona, J. A.Mycobacterium tuberculosis (Mtb), one of the most successful human pathogens in the world, is initially sensed in the lung by pathogen recognition receptors such as TLRs, localized in macrophages, dendritic cells, and monocytes recruited from the bloodstream. This recognition via TLRs initiates a signal transduction pathway in the host cell that culminates with the induction of cytokines and chemokines that are...
TLR2 deficiency by compromising p19 (IL-23) expression limits T helper 17 cell ...
Coelho, Maria Teixeira; Cruz, Andrea; Carmona, J. A.; Sousa, Carole Lara Veiga de; Pereira, Daniela Maria Ramos; Saraiva, Ana Laura; Veldhoen, MarcCD4+ Th1 cells producing IFN-γ are of extreme importance in controlling infections by Mycobacterium tuberculosis both in mice and in men. In addition to IFN-γ-producing T cells, IL-17-producing T cells (Th17) have been observed during mycobacterial infections. Nevertheless, their contribution for the host immune response to mycobacteria as well as the signals triggering M. tuberculosis -specific Th17 cell diffe...
Genome size and ploidy of Paracoccidioides brasiliensis reveals a haploid DNA c...
Almeida, A. J.; Matute, D. R.; Carmona, J. A.; Martins, M.; Torres, I.; McEwen, J. G.; Restrepo, A.; Leão, Cecília; Ludovico, PaulaThe aim of this study was to evaluate genome size and ploidy of the dimorphic pathogenic fungus Paracoccidioides brasiliensis. The cell cycle analysis of 10 P. brasiliensis isolates by flow cytometry (FCM) revealed a genome size ranging from 26.3+/-0.1Mb (26.9+/-0.1fg) to 35.5+/-0.2Mb (36.3+/-0.2fg) per uninucleated yeast cell. The DNA content of conidia from P. brasiliensis ATCC 60855-30.2+/-0.8Mb (30.9+/-0.8f...
Towards a molecular genetic system for the pathogenic fungus Paracoccidiodes br...
Almeida, A. J.; Carmona, J. A.; Cunha, C.; Carvalho, Agostinho; Rappleye, C. A.; Goldman, W. E.; Hooykaas, P. J.; Leão, Cecília; Ludovico, PaulaWe herein report the development of a molecular toolbox for the dimorphic fungus Paracoccidioides brasiliensis, specifically a more efficient transformation and a gene expression system. We evaluated several parameters that influence Agrobacterium tumefaciens-mediated transformation (ATMT), such as co-cultivation conditions and host cell susceptibility. Our results show that cellular recovery and air drying of ...
New insights into the cell cycle profile of Paracoccidioides brasiliensis
Almeida, A. J.; Martins, M.; Carmona, J. A.; Cano, L. E.; Restrepo, A.; Leão, Cecília; Rodrigues, Fernando José dos SantosThe present work focuses on the analysis of cell cycle progression of Paracoccidioides brasiliensis yeast cells under diVerent environmental conditions. We optimized a Xow cytometric technique for cell cycle proWle analysis based on high resolution measurements of nuclear DNA. Exponentially growing cells in poor-deWned or rich-complex nutritional environments showed an increased percentage of daughter cells in ...