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Fuel cells operating as an immunosensor for cancer biomarker screening

Ferreira, Nádia; Carneiro, Liliana; Pinto, Alexandra M. F. R.; Sales, M. Goreti F.

This work presents the first integration of antibodies into one of the electrodes of a passive direct methanol fuel cell (DMFC) to create an autonomous immunosensor for cancer antigen 15–3 (CA15-3). The anode of the fuel cell was first made of carbon cloth with carbon black, platinum and ruthenium nanoparticles and then modified with anti-CA15-3, while the cathode was made of carbon cloth with carbon black and ...



Time windows vehicle routing problem to on-time transportation of biological pr...

Pereira, Maria Teresa; Oliveira, Marisa; Ferreira, Fernanda A.; Barreiras, Alcinda; Carneiro, Liliana

This paper addresses a Vehicle Routing Problem (VRP) applied to the field of healthcare. Biological products are collected from patients at the local healthcare centers and transported to hospital laboratories for further processing and analysis. This paper analyses and determines a set of vehicle routes to perform on-time transportation of biological products from local healthcare centers to the main hospital,...


Fuel cells operating as an immunosensor for cancer biomarker screening

Ferreira, Nádia; Carneiro, Liliana; Pinto, Alexandra M. F. R.; Sales, M. G. F.

This work presents the first integration of antibodies into one of the electrodes of a passive direct methanol fuel cell (DMFC) to create an autonomous immunosensor for cancer antigen 153 (CA15-3). The anode of the fuel cell was first made of carbon cloth with carbon black, platinum and ruthenium nanoparticles and then modified with anti-CA15-3, while the cathode was made of carbon cloth with carbon black and p...


Development of an innovative flexible paper-based methanol fuel cell (PB-DMFC) ...

Carneiro, Liliana; Pinto, Alexandra M. F. R.; Sales, M. G. F.

This work describes for the first time a paper-based direct methanol fuel cell platform (PB-DMFC) that functions as an energy source and biosensor, assembled on a simple paper substrate for point-of-care (POC) applications, targeting sarcosine as proof-of-concept. Specifically, a methanol fuel cell strip was developed from a square of Whatman paper, acting as substrate. The paper strip was treated with an imper...


An all-in-one approach for self-powered sensing: a methanol fuel cell modified ...

Carneiro, Liliana; Pinto, Alexandra M. F. R.; Mendes, Adélio; Sales, M. G. F.

This work describes the development of an innovative electrochemical biosensor comprehending a passive direct methanol fuel cell (DMFC) assembly, modified by a layer of a molecularly imprinted polymer (MIP) on a carbon fabric anode electrode containing Pt/Ru nanoparticles, This MIP film was prepared from poly(3,4-ethylenedioxythiophene) (PEDOT) and polypyrrole (PPy) obtained by in situ electropolymerization of ...


A passive direct methanol fuel cell as transducer of an electrochemical sensor,...

Carneiro, Liliana; Ferreira, Nádia; Moreira, Ana; Pinto, Alexandra M. F. R.; Mendes, Adélio; Sales, M. G. F.

This work describes an electrochemical sensor with a biomimetic plastic antibody film for carcinoembryonic antigen (CEA, an important biomarker in colorectal cancer), integrated in the electrical circuit of a direct methanol fuel cell (DMFC), working in passive mode and used herein as power supply and signal transducer. In detail, the sensing layer for CEA consisted of a Fluorine-doped Tin Oxide (FTO) conductiv...


A new vision for fuel cells: application in cancer biomarker screening

Carneiro, Liliana; Pinto, Alexandra M. F. R.; Sales, M. G. F.


Employing bacteria machinery for antibiotic detection: using DNA gyrase for cip...

Cardoso, Rita; Carneiro, Liliana; Cabral-Miranda, Gustavo; Bachmann, Martin F.; Sales, M. G. F.

This work describes a new successful approach for designing biosensors that detect antibiotics. It makes use of a biomimetic strategy, by employing the biochemical target of a given antibiotic as its biorecognition element. This principle was tested herein for quinolones, which target DNA gyrase in bacteria. Ciprofloxacin (CIPRO) was tested as a representative antibiotic from the quinolone group; the sensitivit...


Development of a biossensor for prostate cancer using sarcosine as biomarker

Almeida, Maria; Carneiro, Liliana; Sales, M. Goreti

Prostate cancer is the most common type of tumor disease in men, making the development of new methods that allow an earlier detection extremely important. One of these methods concerns the use of biosensors to diagnose specific biomarkers for this type of cancer. Biomarkers could be amino acids, proteins or nucleic acids. In this work, the amino acid sarcosine was selected for biosensor development, making use...


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