8 documents found, page 1 of 1
Transcriptional responses of Hypericum perforatum cells to Agrobacterium tumefa...
Hou, Weina; Singh, Rupesh K.; Martins, Viviana; Tenllado, Francisco; Gregory, Franklin; Dias, AlbertoHypericum perforatum L. (St. John's wort) is a well-known medicinal plant that possesses secondary metabolites with beneficial pharmacological properties. However, improvement in the production of secondary metabolites via genetic manipulation is a challenging task as H. perforatum remains recalcitrant to Agrobacterium tumefaciens-mediated transformation. Here, the transcripts of key genes involved in several p...
A perspective on Hypericum perforatum Genetic transformation
Hou, Weina; Shakya, Preeti; Gregory, FranklinHypericum perforatum (St John's wort) is a reservoir of diverse classes of biologically active and high value secondary metabolites, which captured the interest of both researchers and the pharmaceutical industry alike. Several studies and clinical trials have shown that H. perforatum extracts possess an astounding array of pharmacological properties. These properties include antidepressant, anti-inflammatory, ...
Intellectual property landscape of genetically modified plants in Europe
Gregory, FranklinIn spite of strict policies and regulations on genetically modified (transgenic) crops in the European Union (EU), there is still considerable commercial interest in this area and the associated intellectual property rights. In this thesis, the current scenario of protecting genetically modified plants in the EU is discussed based on the existing legal regime, case decisions of European Patent Office (EPO) and ...
Signaling pathways influencing tumor microenvironment and their exploitation fo...
Singh, Caroline Jeya Sheeba Daniel Sunder; Marslin, Gregory; Mary, Revina Ann; Gregory, FranklinIn the recent years, the “tumor microenvironment” has been receiving growing attention due to its involvement in neoplastic transformation, tumor growth, invasion and protection of tumor cells from host immune response. All these events are facilitated by chemical signals produced by the tumor as well as the surrounding stromal cells. This review is divided into two main parts in which the first part discusses ...
In vitro flowering and viable seed setting of transgenic lettuce cultures
Gregory, Franklin; Oliveira, Ana Lucia; Dias, Alberto Carlos PiresProduction of transgenic lettuce seeds via in vitro flowering and fruit setting is reported here. Six days old cotyledons were co-cultivated with Agrobacterium tumefaciens strain EHA105 harbouring the binary vector pCAMBIA2301 carrying the reporter gene a-glucuronidase intron (GUS-INT) and the marker gene neomycin phosphotransferase (NPTII). Transgenic calluses and shoot buds were induced on MS medium augmented ...
Transgenic hypericum perforatum
Gregory, Franklin; Oliveira, Margarida M.; Dias, Alberto Carlos PiresPlant transformation is an important tool with many applications in modern plant biology. Although this technique is primarily used to produce superior crop varieties, it is also being utilized to answer basic questions concerning gene function and regulation in contemporary functional genomics research. In our laboratory, we have established a transformation system for Hypericum perforatum . This protocol invo...
Poly(D,L-lactic-co-glycolic acid) nanoencapsulation reduces erlotinib-Induced s...
Gregory, Marslin; Sheeba, Caroline J.; Kalaichelvan, V. K.; Manavalan, R.; Reddy, Neelakanta; Gregory, FranklinErlotinib-HCl is a quinazoline derivative used as a drug in the therapy of non-small-cell lung cancer. The present study was conducted to compare the subacute toxicity induced by Erlotinib-HCl delivered to rats as nanoparticles and as free drug. Wistar rats were orally administered with a daily dosage of 200 mg kg−1 Erlotinib-HCl either as free drug or as Poly(D,L-lactic-co-glycolic acid) (PLGA) encapsulated na...
Anti-ulcer (ulcer-preventive) activity of ficus arnottiana miq. (moraceae) leaf...
Gregory, Marslin; Vithalrao, K. P.; Gregory, Franklin; Kalaichelvan, V. K.Problem statement: In spite of being one of the well-known medicinal plants used in Indian traditional medicine to treat several ailments, studies pertaining to the pharmacological properties of Ficus arnottiana are very scarce. We studied the anti-ulcer activity and acute toxicity of Ficus arnottiana leaf methanolic extract for the first time. Approach: Freshly collected F. arnottiana leaves were dried, powder...