6 documents found, page 1 of 1
Metabolite secretion in microorganisms: the theory of metabolic overflow put to...
Pinu, Farhana R.; Granucci, Ninna; Daniell, James; Han, Ting-Li; Carneiro, Sónia; Rocha, I.; Nielsen, Jens; Villas-Boas, Silas G.Introduction Microbial cells secrete many metabolites during growth, including important intermediates of the central carbon metabolism. This has not been taken into account by researchers when modeling microbial metabolism for metabolic engineering and systems biology studies. Materials and Methods The uptake of metabolites by microorganisms is well studied, but our knowledge of how and why they secrete differ...
Integrated metabolic flux and omics analysis of leishmania major metabolism
Shakyawar, S.; Rocha, I.; Carneiro, SóniaLeishmaniasis is a virulent parasitic infection that causes a significant threat to human health worldwide. The existing drugs are becoming less effective due to the ability of Leishmania spp. to alter its metabolism to adapt to harsh environments. Understanding how this parasite manipulates its metabolism inside the host (e.g. sandfly and human) might underpin new ways to prevent the disease and develop effect...
Reconstruction of a genome-scale metabolic model for Actinobacillus succinogene...
Pereira, Bruno; Miguel, Joana; Vilaça, Paulo; Soares, Simão; Rocha, I.; Carneiro, SóniaBackground Actinobacillus succinogenes is a promising bacterial catalyst for the bioproduction of succinic acid from low-cost raw materials. In this work, a genome-scale metabolic model was reconstructed and used to assess the metabolic capabilities of this microorganism under producing conditions. Results The model, iBP722, was reconstructed based on the functional reannotation of the complete genome sequence ...
Reconstruction of a genome-scale metabolic model for Actinobacillus succinogenes
Carneiro, Sónia; Miguel, Joana; Carreira, Rafael; Vilaça, Paulo Ricardo Carvalho; Rocha, I.Actinobacillus succinogenes, a gram-negative bacterium, is one the most promising natural producers of succinate. This chemical has been well established as a bio-based chemical platform to produce bulk chemicals (e.g. 1,4-butanediol) and other biomaterials, but the costs associated with the bioproduction of succinate are still discouraging. One of the reasons is that succinate is often produced together with o...
Assessment of physiological conditions in e. Coli fermentations by epifluoresce...
Carneiro, Sónia; Amaral, António L.; Veloso, Ana C.A.; Dias, Teresa; Peres, António M.; Ferreira, Eugénio C.; Rocha, IsabelThe development of monitoring methods for assessing the physiological state of microorganisms during recombinant fermentation processes has been encouraged by the need to evaluate the influence of processing conditions in recombinant protein production. In this work, a technique based on microscopy and image analysis was developed that allows the simultaneous quantification of parameters associated with viabili...
The @Note biomedical text mining workbench
Lourenço, Anália; Carreira, Rafael; Carneiro, Sónia; Maia, Paulo; Ferreira, Eugénio C.; Glez-Peña, D.; Fdez-Riverola, F.; Rocha, Miguel; Rocha, I.[Excerpt] Biomedical Text Mining. i.e., the field that deals with the automatic: retrieval and processing of biomedical literature, is perhaps one of today's most promising research fields. The automated processing of free text is not exactly a new computational issue. Text Mining has a long experience in the retrieval and processing of general text. The development of search engines and indexed directories, th...