39 documents found, page 1 of 4
MicroRNA-186-5p controls GluA2 surface expression and synaptic scaling in hippo...
Silva, Mariline M.; Rodrigues, Beatriz; Fernandes, Joana; Santos, Sandra D.; Carreto, Laura; Santos, Manuel A. S.; Pinheiro, Paulo; Carvalho, Ana LuísaHomeostatic synaptic scaling is a negative feedback response to fluctuations in synaptic strength induced by developmental or learning-related processes, which maintains neuronal activity stable. Although several components of the synaptic scaling apparatus have been characterized, the intrinsic regulatory mechanisms promoting scaling remain largely unknown. MicroRNAs may contribute to posttranscriptional contr...
Synthetic microparticles conjugated with VEGF165 improve the survival of endoth...
Aday, Sezin; Zoldan, Janet; Besnier, Marie; Carreto, Laura; Saif, Jaimy; Fernandes, Rui; Santos, Tiago; Bernardino, Liliana Inácio; Langer, RobertSeveral cell-based therapies are under pre-clinical and clinical evaluation for the treatment of ischemic diseases. Poor survival and vascular engraftment rates of transplanted cells force them to work mainly via time-limited paracrine actions. Although several approaches, including the use of soluble vascular endothelial growth factor (sVEGF)-VEGF165, have been developed in the last 10 years to enhance cell su...
The RNA-Binding Protein hnRNP K Mediates the Effect of BDNF on Dendritic mRNA M...
Leal, Graciano; Comprido, Diogo; Luca, Pasqualino De; Morais, Eduardo Manuel Firmo; Rodrigues, Luís; Mele, Miranda; Santos, Ana R.; Costa, Rui O.Brain-derived neurotrophic factor (BDNF) is an important mediator of long-term synaptic potentiation (LTP) in the hippocampus. The local effects of BDNF depend on the activation of translation activity, which requires the delivery of transcripts to the synapse. In this work, we found that neuronal activity regulates the dendritic localization of the RNA-binding protein heterogeneous nuclear ribonucleoprotein K ...
Adaptive mistranslation accelerates the evolution of fluconazole resistance and...
Weil, Tobias; Santamaría, Rodrigo; Lee, Wanseon; Rung, Johan; Tocci, Noemi; Abbey, Darren; Bezerra, Ana R.; Carreto, Laura; Moura, Gabriela R.Regulated erroneous protein translation (adaptive mistranslation) increases proteome diversity and produces advantageous phenotypic variability in the human pathogen Candida albicans. It also increases fitness in the presence of fluconazole, but the underlying molecular mechanism is not understood. To address this question, we evolved hypermistranslating and wild-type strains in the absence and presence of fluc...
The RNA-binding protein hnRNP K mediates the effect of BDNF on dendritic mRNA m...
Leal, Graciano; Comprido, Diogo; Luca, Pasqualino de; Morais, Eduardo; Rodrigues, Luís; Mele, Miranda; Santos, Ana R.; Costa, Rui O.; Pinto, Maria JoanaBrain-derived neurotrophic factor (BDNF) is an important mediator of long-term synaptic potentiation (LTP) in the hippocampus. The local effects of BDNF depend on the activation of translation activity, which requires the delivery of transcripts to the synapse. In this work, we found that neuronal activity regulates the dendritic localization of the RNA-binding protein heterogeneous nuclear ribonucleoprotein K ...
Helicobacter pylori activates matrix metalloproteinase 10 in gastric epithelial...
Costa, Angela M.; Ferreira, Rui M.; Pinto-Ribeiro, Inês; Sougleri, Ioanna S.; Oliveira, Maria J.; Carreto, Laura; Santos, Manuel A.Helicobacter pylori colonizes the human stomach and increases the risk for peptic ulcer disease and gastric carcinoma. H. pylori upregulates the expression and activity of several matrix metalloproteinases (MMPs) in cell lines and in the gastric mucosa. The aim of this study was to explore the mechanisms leading to upregulation of MMP10 in gastric epithelial cells induced by H. pylori Infection of gastric cells...
Intrastrain genomic and phenotypic variability of the commercial Saccharomyces ...
Franco-Duarte, Ricardo; Bigey, Frederic; Carreto, Laura; Mendes, Inês Isabel Moreira Moutinho Vieira; Dequin, Sylvie; Santos, Manuel A. S.; Pais, CéliaThe maintenance of microbial species in different environmental conditions is associated with adaptive microevolutionary changes that are shown here to occur within the descendants of the same strain in comparison with the commercial reference strain. However, scarce information is available regarding changes that occur among strain descendants during their persistence in nature. Herein we evaluate genome varia...
Intrastrain genomic and phenotypic variability of the commercial Saccharomyces ...
Franco-Duarte, Ricardo; Bigey, Frédéric; Carreto, Laura; Mendes, Inês; Dequin, Sylvie; Santos, Manuel A. S.; Pais, Célia; Schuller, DoritThe maintenance of microbial species in different environmental conditions is associated with adaptive microevolutionary changes that are shown here to occur within the descendants of the same strain in comparison with the commercial reference strain. However, scarce information is available regarding changes that occur among strain descendants during their persistence in nature. Herein we evaluate genome varia...
In vitro ischemia triggers a transcriptional response to down-regulate synaptic...
Fernandes, Joana; Vieira, Marta; Carreto, Laura; Santos, Manuel A. S.; Duarte, Carlos B.; Carvalho, Ana Luísa; Santos, Armanda E.Transient global cerebral ischemia induces profound changes in the transcriptome of brain cells, which is partially associated with the induction or repression of genes that influence the ischemic response. However, the mechanisms responsible for the selective vulnerability of hippocampal neurons to global ischemia remain to be clarified. To identify molecular changes elicited by ischemic insults, we subjected ...
Participation of Candida albicans transcription factor Rlm1 in cell wall biogen...
Silva, Yolanda Bedsabé Delgado; Vaz, Catarina Oliveira; Pereira, Joana Carvalho; Carneiro, Catarina; Nogueira, Eugénia Sofia Costa; Correia, AlexandraCandida albicans cell wall is important for growth and interaction with the environment. RLM1 is one of the putative transcription factors involved in the cell wall integrity pathway, which plays an important role in the maintenance of the cell wall integrity. In this work we investigated the involvement of RLM1 in the cell wall biogenesis and in virulence. Newly constructed C. albicans Δ/Δrlm1 mutants showed t...