3 documents found, page 1 of 1
Toxicological impact of acute exposure to E171 food additive and TiO(2) nanopar...
Dorier, M; Tisseyre, C; Dussert, F; Béal, D; Arnal, ME; Douki, T; Valdiglesias, V; Laffon, B; Fraga, S; Brandão, F; Herlin-Boime, N; Barreau, FTiO2 particles are widely used in products for everyday consumption, such as cosmetics and food; their possible adverse effects on human health must therefore be investigated. The aim of this study was to document in vitro impact of the food additive E171, i.e. TiO2, and of TiO2 nanoparticles, on a co-culture of Caco-2 and HT29-MTX cells, which is an in vitro model for human intestine. Cells were exposed to TiO...
Optimization of the harvesting and freezing conditions of human cell lines for ...
Bessa, MJ; Brandão, F; Querido, MM; Costa, C; Pereira, CC; Valdiglesias, V; Laffon, B; Carriere, M; Teixeira, JP; Fraga, SThe comet assay is a commonly used method for in vitro and in vivo genotoxicity assessment. This versatile assay can be performed in a wide range of tissues and cell types. Although most of the studies use samples immediately processed after collection, frozen biological samples can also be used. The present study aimed to optimize a collection and freezing protocol to minimize the DNA damage associated with th...
Assessment of oxidative damage induced by iron oxide nanoparticles on different...
Fernández-Bertólez, N; Costa, C; Bessa, MJ; Park, M; Carriere, M; Dussert, F; Teixeira, JP; Pásaro, E; Laffon, B; Valdiglesias, VIron oxide nanoparticles (ION) have received much attention for their utility in biomedical applications, such as magnetic resonance imaging, drug delivery and hyperthermia, but concerns regarding their potential harmful effects are also growing. Even though ION may induce different toxic effects in a wide variety of cell types and animal systems, there is a notable lack of toxicological data on the human nervo...