30 documents found, page 1 of 3

Tetracycline and rifampicin induced a viable but nonculturable state in Staphyl...

AIM: The goal of this study was to determine the effectiveness of antibiotics on Staphylococcus epidermidis biofilms with different proportions of dormant bacteria, using clinical and commensal isolates. MATERIALS & METHODS: The ability of S. epidermidis isolates to develop a dormant state was determined. The susceptibility of biofilms with prevented or induced dormancy to antibiotics was evaluated by enumerati...

Towards Staphylococcus epidermidis biofilm dormancy characterization

Biofilm dormancy enhances antimicrobial tolerance in S. epidermidis

Indwelling medical devices have been increasingly used in modern medicine and have saved millions of lives worldwide. However, they can also be an important source of infections, most commonly caused by coagulase negative-staphylococci, particularly by biofilm forming Staphylococcus epidermidis. A key feature of biofilms is its enhanced tolerance to antibiotics. Several mechanisms have been proposed to contribu...

S. epidermidis lifecycle: how bacteria trick the host and the clinician?

Background: In the clinical setting, biofilms were thought to be an alternative phenotype to planktonic pure cultures but scientific evidence from the last decade has revealed that bacteria, similar to more evolved species, undergo a specific lifecycle containing: (i) biofilm formation (ii) dispersion and (iii) planktonic growth phases. This results in constant alterations in bacterial physiology, with signific...

Characterization of an in vitro fed-batch model to obtain cells released from S...

Both dynamic and fed-batch systems have been used for the study of biofilms. Dynamic systems, whose hallmark is the presence of continuous flow, have been considered the most appropriate for the study of the last stage of the biofilm lifecycle: biofilm disassembly. However, fed-batch is still the most used system in the biofilm research field. Hence, we have used a fed-batch system to collect cells released fro...

S. epidermidis biofilm-released cells: the final frontier?

Poly-N-acetylglucosamine production by Staphylococcus epidermidis cells increas...

Poly-N-acetyl glucosamine (PNAG) is a major component of Staphylococcus epidermidis biofilms extracellular matrix. However, it is not yet clear how this polysaccharide impacts the host immune response and infection-associated pathology. Faster neutrophil recruitment and bacterial clearance was observed in mice challenged intraperitoneally with S. epidermidis biofilm cells of the PNAG-producing 9142 strain than ...

An immunoproteomic approach for characterization of dormancy within Staphylococ...

Virulence of Staphylococcus epidermidis is mainly attributed to surface colonization and biofilm formation in indwelling medical devices. Physiological heterogeneity of biofilms may influence host immune response and sensitivity to antibiotics. Dormant cells, among others, contribute to biofilm heterogeneity. The aim of this study was to identify immunogenic proteins of S. epidermidis biofilms associated with d...

Immunoreactive pattern of Staphylococcus epidermidis biofilm against human whol...

Saliva is essential to interact with microorganisms in the oral cavity. Therefore, the interest in saliva antimicrobial properties is on the rise. Here, we used an immunoproteomic approach, based on protein separation of Staphylococcus epidermidis biofilms by 2DE, followed by Western-blotting, to compare human serum and saliva reactivity profile. A total of 17 proteins were identified by MALDI-TOF/TOF. Serum an...

Dormancy within Staphylococcus epidermidis biofilms: an immunoproteomic charact...

Dormant bacteria within biofilms contribute to biofilm heterogeneity. Consequently, physiological heterogeneity of biofilms may influence host immune response and tolerance to antibiotics. Recently, we described an in vitro model to modulate dormancy in S. epidermidis biofilms. Here, we present a study based on immunoproteomics, where we compared the reactive profile of S. epidermidis biofilm proteins with prev...