89 documents found, page 1 of 9
Influence of Inhibitors Generated in Lignocellulosic Hydrolysates from Group of...
Gaspar, Suelen S.; Alves Ferreira Caturra, Júnia Aparecida; Moniz, Patricia; Silva-Fernandes, Talita; Silvestre, Adriana I. R; Torrado, IvoneABSTRACT: Concerns over fossil fuels are of increasing interest in biorefineries that utilize lignocellulosic residues. Besides sugars, inhibitors are formed during biomass pretreatment, including acetic acid (AI) and formic acid (FI), which can hinder microbial fermentation. The TG1 and Tuner strains of Escherichia coli were subjected to various acid concentrations. Samples were taken during fermentation to mo...
Fractionation of macroalgae carbohydrates using hydrothermal and dilute inorgan...
Martins, Pedro L.; Andrade, Cristiana; Duarte, Luís; Reis, Alberto; Pereira, Helena; Carvalheiro, FlorbelaABSTRACT: Furans are among the most important compounds derived from biomass, providing conversion pathways for sustainable alternatives to petroleum-based fuels and materials. Furfural, 5-hydroxymethylfurfural (5-HMF), and 5-methylfurfural (5-MF) are furans that can be obtained by carbohydrate dehydration under acidic conditions at elevated temperature and pressure. One of the mechanisms to produce these compo...
Microwave-assisted hydrothermal processing of pine nut shells for oligosacchari...
Torrado, Ivone; Neves, Beatriz Guapo; Fernandes, M. C.; Carvalheiro, Florbela; Pereira, Helena; Duarte, Luís C.ABSTRACT: Pine nut shells, a biomass residue from the Mediterranean Pinus pinea pine nut industrial processing, were treated by microwave-assisted autohydrolysis to produce xylo-oligosaccharides. Microwave-assisted processes provide alternative heating that may reduce energy input and increase overall process efficiency. The autohydrolysis treatments were performed under isothermal and non-isothermal operations...
Evaluation of different fractionation methods for the simultaneous protein and ...
Martins, Pedro L.; Duarte, Luís C.; Pereira, Helena; Reis, Alberto; Carvalheiro, FlorbelaABSTRACT: The production of high-value products from microalgae, one of the preferred emerging biorefineries' feedstocks, relies on the crucial step of biomass fractionation. In this work, the fractionation of Chlorella vulgaris and Scenedesmus obliquus biomass was tested for protein extraction using a wide range of physical, chemical, and enzymatic treatment combinations, including ultrasound, cell homogenizer...
Microwave-assisted hydrothermal processing of pine nut shells for oligosacchari...
Torrado, Ivone; Neves, Beatriz Guapo; Fernandes, Maria da Conceição; Carvalheiro, Florbela; Pereira, Helena; Duarte, Luís C.Pine nut shells, a biomass residue from the Mediterranean Pinus pinea pine nut industrial processing, were treated by microwave- assisted autohydrolysis to produce xylo-oligosaccharides. Microwave-assisted processes provide alternative heating that may reduce energy input and increase overall process efficiency. The autohydrolysis treatments were performed under isothermal and non-isothermal operations within a...
Evaluation of different fractionation methods for the simultaneous protein and ...
Martins, Pedro L.; Duarte, Luís C.; Pereira, Helena; Reis, Alberto; Carvalheiro, FlorbelaThe production of high-value products from microalgae, one of the preferred emerging biorefineries’ feedstocks, relies on the crucial step of biomass fractionation. In this work, the fractionation of Chlorella vulgaris and Scenedesmus obliquus biomass was tested for protein extraction using a wide range of physical, chemical, and enzymatic treatment combinations, including ultrasound, cell homogenizer, cellulas...
Lignin utilization for the removal of microplastic particles from water
Găgeanu, Iuliana; Carvalheiro, Florbela; Ekielski, Adam; Duarte, Luís C.ABSTRACT: The focus of the research was to evaluate the use of lignin from different sources as an agent for the removal of diverse types of microplastics when present in wastewater. Organosolv lignin was obtained from three different sources (Miscanthus sp., pine bark and solid anaerobic digestates from Organic Fraction of Municipal Solid Wastes) by an ethanol-based organosolv treatment carried out in a pressu...
Cistus ladanifer as a potential feedstock for biorefineries: A review
Alves-Ferreira, Júnia; Duarte, Luís C.; Fernandes, Maria C.; Pereira, Helena; Carvalheiro, FlorbelaCistus ladanifer (rockrose) is a widespread shrub species in the Mediterranean region well known due to its production of labdanum gum, especially in the hot season. Its leaves and branches can be subjected to different extraction and distillation processes to produce various types of extracts. The natural extracts of C. ladanifer have several applications, especially in the perfumery and cosmetics sector. C. l...
The Role of Mild Alkaline Pretreatment in the Biorefinery Upgrade of Spent Coff...
Ribeiro, Gabriel Mota; Martins, Pedro L.; Oliveira, Ana Cristina; Carvalheiro, Florbela; Fragoso, Rita; Duarte, Luís C.ABSTRACT: This work proposes a valorization route for spent coffee grounds (SCG), a widespread lignocellulosic residue, encompassing the production of: biomethane, lignin, and oligosaccharides as value-added products obtained simultaneously during a mild alkaline (NaOH) pretreatment. The studied operational variables were the reaction time (60-240 min), temperature (25-75 degrees C), and the NaOH concentration ...
Visible light-exposed lignin facilitates cellulose solubilization by lytic poly...
Kommedal, Eirik G.; Angeltveit, Camilla F.; Klau, Leesa J.; Ayuso-Fernández, Iván; Arstad, Bjørnar; Antonsen, Simen G.; Stenstrøm, Yngve; Ekeberg, DagABSTRACT: Degradation of plant biomass, comprised of cellulose and polyaromatic lignin, is promoted by light. Here, the authors show that light promotes lignin-catalyzed generation of hydrogen peroxide, which is used by redox enzymes to degrade cellulose. Lytic polysaccharide monooxygenases (LPMOs) catalyze oxidative cleavage of crystalline polysaccharides such as cellulose and are crucial for the conversion of...