9 documents found, page 1 of 1
Reduced numbers of regulatory T cells in chronic heart failure seems not to be ...
Martins, Sílvia; António, Natália; Carvalheiro, Tiago; Laranjeira, Paula; Rodrigues, Ricardo; Gonçalves, Lino; Tomaz, Cândida; Paiva, ArturBackground T cells have been implicated in the development and progression of inflammatory processes in chronic heart failure (CHF). Cardiac resynchronization therapy (CRT) has beneficial effects on symptoms and cardiac remodeling in CHF. However, its impact on the inflammatory immune response remains controversial. We aimed to study the impact of CRT on T cells in heart failure (HF) patients. Methods Thirty-ni...
Impact of cardiac resynchronization therapy on circulating IL-17 producing cell...
Martins, Sílvia; Carvalheiro, Tiago; Laranjeira, Paula; Martinho, António; Elvas, Luís; Gonçalves, Lino; Tomaz, C. T.; António, Natália; Paiva, ArturIL-17-producing T cells have been implicated in the inflammatory milieu of chronic heart failure (CHF), which implies a dismal prognosis in affected patients. The aim of this study was to evaluate the impact of cardiac resynchronization therapy (CRT) on the frequency and functional activity of Th17 and Tc17 cells, as well as, on IL-17 mRNA expression in patients with CHF.
Frequency and functional activity of Th17, Tc17 and other T-cell subsets in Sys...
Henriques, Ana; Inês, Luís; Couto, Maura; Pedreiro, Susana; Santos, Catarina; Magalhães, Mariana; Santos, Paulo; Velada, Isabel; Almeida, AnabelaTo compare frequency and functional activity of peripheral blood (PB) Th(c)17, Th(c)1 and Treg cells and the amount of type 2 cytokines mRNA we recruited SLE patients in active (n = 15) and inactive disease (n = 19) and healthy age- and gender-matched controls (n = 15). The study of Th(c)17, Th(c)1 and Treg cells was done by flow cytometry and cytokine mRNA by real-time PCR. Compared to NC, SLE patients present...
Oxidative Stress, DNA, Cell Cycle/Cell Cycle Associated Proteins and Multidrug ...
Mamede, Ana Catarina Manjolinha; Guerra, S; Laranjo, Marta; Santos, K; Carvalho, Maria J; Carvalheiro, Tiago; Moura, P; Paiva, A; Abrantes, Ana MThe anticancer effects of human amniotic membrane (hAM) have been studied over the last decade. However, the action mechanisms responsible for these effects are not fully understood until now. Previously results reported by our team proved that hAM is able to induce cytotoxicity and cell death in hepatocellular carcinoma (HCC), a worldwide high incident and mortal cancer. Therefore, this experimental study aime...
Sera from patients with active systemic lupus erythematosus patients enhance th...
Carvalheiro, Tiago; Gomes, Diane; Pinto, Ligia A; Inês, Luís; Lopes, Ana; Henriques, Ana; Pedreiro, Susana; Martinho, António; Trindade, HélderBackground: Systemic Lupus Erythematosus (SLE) is an auto-immune disease whose complex pathogenesis remains unraveled. Here we aim to explore the inflammatory ability of SLE patients’ sera upon peripheral blood (PB) monocyte subsets and myeloid dendritic cells (mDCs) obtained from healthy donors. Methods: In this study we included 11 SLE patients with active disease (ASLE), 11 with inactive disease (ISLE) and 1...
Lysophosphatidic acid enhances survival of human CD34(+) cells in ischemic cond...
Kostic, Ivana; Fidalgo-Carvalho, Isabel; Aday, Sezin; Vazão, Helena; Carvalheiro, Tiago; Grãos, Mário; Duarte, António; Cardoso, Carla; Gonçalves, LinoSeveral clinical trials are exploring therapeutic effect of human CD34(+) cells in ischemic diseases, including myocardial infarction. Unfortunately, most of the cells die few days after delivery. Herein we show that lysophosphatidic acid (LPA)-treated human umbilical cord blood-derived CD34(+) cells cultured under hypoxic and serum-deprived conditions present 2.2-fold and 1.3-fold higher survival relatively to...
Reduced levels of circulating endothelial progenitor cells in acute myocardial ...
António, Natália; Fernandes, Rosa; Soares, Ana; Soares, Francisco; Lopes, Ana; Carvalheiro, Tiago; Paiva, Artur; Pêgo, Guilherme M.Background: Diabetic patients have a significantly worse prognosis after an acute myocardial infarction (AMI) than their counterparts. Previous studies have shown that the number of circulating endothelial progenitor cells (EPCs) significantly increase early after an AMI in normoglycemic patients. However, it is well known that type 2 diabetes mellitus (DM) is associated with impaired function and reduced circu...
Endothelial progenitor cells in diabetic patients with myocardial infarction – ...
António, Natália; Soares, Ana; Fernandes, Rosa; Soares, Francisco; Lopes, Ana; Carvalheiro, Tiago; Paiva, Artur; Providência, Luís A.; Gonçalves, LinoThe effect of statins on endothelial progenitor cells (EPCs) function derived from diabetic patients (DMpts) with acute myocardial infarction (AMI) is unknown. In this study we assess the response of early and late EPCs from diabetic versus non-diabetic patients (NDMpts) with AMI to statins. EPCs were obtained from 10 diabetic and 10 age-matched non-diabetic male patients with AMI. For each patient, cultures of...
Tolerogenic versus inflammatory activity of peripheral blood monocytes and dend...
Carvalheiro, Tiago; Rodrigues, Ana; Lopes, Ana; Inês, Luís; Velada, Isabel; Ribeiro, Andreia; Martinho, António; Silva, José A. P.; Pais, Maria L.Abnormalities in monocytes and in peripheral blood dendritic cells (DC) subsets have been reported in systemic lupus erythematosus (SLE). We aim to clarify the tolerogenic or inflammatory role of these cells based on ICOSL or IFN-α and chemokine mRNA expression, respectively, after cell purification. The study included 18 SLE patients with active disease (ASLE), 25 with inactive disease (ISLE), and 30 healthy c...